Chemical and biological evaluation of five endemic Salvia species: Rich source of rosmarinic, caffeic, fumaric and malic acids

Serkan Yiğitkan; Leyla Balur Adsız; Mustafa Abdullah Yılmaz; Evin Aygün; Hilal Saruhan Fidan; Esra Yariş; Mehmet Akdeniz; Mehmet Veysi Çağlayan; İsmail Yener; Mehmet Boğa; Mehmet Fırat; Ufuk Kolak; Abdulselam Ertaş

doi:10.18016/ksutarimdoga.vi.1804808

TR EN

Beş endemik Salvia türünün kimyasal ve biyolojik değerlendirmesi: Rosmarinik, kafeik, fumarik ve malik asitlerin zengin kaynağı

Öz

Tıbbi bitkiler eski çağlardan beri birçok hastalığın tedavisinde kullanılmış olup, sinerjik etkileri ve sentetik ilaçların ciddi yan etkileri nedeniyle toplumda bitkilerle tedavi giderek daha fazla tercih edilmektedir. Bu çalışmada, halk arasında yaygın olarak kullanılan Salvia cinsine ait 5 endemik türün (Salvia cerino-pruinosa var. elazigensis, S. cerino-pruinosa var. cerino-pruinosa, S. rosifolia, S. pseudeuphratica ve S. kurdica) fenolik içerikleri LC-MS/MS yöntemi ile belirlenmiş ve antioksidan, sitotoksik, anti-Alzheimer, antiüreaz, antitirozinaz, antielastaz ve antikolejenaz aktiviteleri araştırılmıştır. Türün karışık kısımlarından hazırlanan petrol eteri özütleri fenolik içerik bakımından en fakir, etanol özütleri ise en zengin olarak bulunmuştur. Dolayısıyla örnek özütlerin hazırlanması sırasında çözücünün polaritesi arttıkça, özütlerin fenolik bileşen çeşitliliği bakımından daha zengin hale geldiği gözlenmiştir. Genel olarak tüm etanol özütlerinin rosmarinik asit, kafeik asit, fumarik asit ve malik asit açısından zengin olduğu bulunmuştur. Özellikle S. cerino-pruinosa var. elazigensis diğer türlere kıyasla bileşen çeşitliliği ve miktarları bakımından daha zengin bulunmuştur. Özellikle bu türün yaprak kısmının rosmarinik asit (34638,45 µg analit/g özüt) içeriği dikkat çekicidir. Genel olarak tüm türlerin antioksidan aktiviteleri yüksek veya orta düzeyde, enzim aktiviteleri ise düşük bulunmuştur. Bu çalışma, 5 endemik türün çeşitli kısımlarının farklı çözücüler (petrol eteri, kloroform ve etanol) kullanılarak hazırlanan ekstraktlarının kimyasal içerikleri ve biyolojik aktiviteleri üzerine literatürde yapılan ilk detaylı çalışmadır. S. cerino-pruinosa var. elazigensis yaprağının etanol ekstraktı yüksek toplam fenolik ve rosmarinik asit içeriğine ve antioksidan potansiyele sahiptir ve S. rosifolia'nın sitotoksik aktivitesi, gıda takviyesi, gıda koruyucu ve ilaç endüstrilerinde kullanım potansiyeline sahiptir.

Anahtar Kelimeler

Chemical and biological evaluation of five endemic Salvia species: Rich source of rosmarinic, caffeic, fumaric and malic acids

Abstract

Medicinal plants have been used to treat many diseases since ancient times, and treatment with herbs is becoming more and more preferred in society due to their synergistic effects and the serious side effects of synthetic drugs. In this study, the phenolic contents of 5 endemic species (Salvia cerino-pruinosa var. elazigensis, S. cerino-pruinosa var. cerino-pruinosa, S. rosifolia, S. pseudeuphratica and S. kurdica) belonging to the genus Salvia, which are widely used among the public, were determined by LC-MS/MS and their antioxidant, cytotoxic, anti-Alzheimer, urease, tyrosinase, elastase and collegenase activities were investigated. Petroleum ether extracts prepared from mixed parts of the species were found to be the poorest in terms of phenolic content, while ethanol extracts were the richest. Therefore, as the polarity of the solvent increased during the preparation of the sample extracts, it was observed that the extracts became richer in terms of phenolic component diversity. In general, all ethanol extracts were found to be rich in rosmarinic acid, caffeic acid, fumaric acid and malic acid. Especially S. cerino-pruinosa var. elazigensis was found to be richer in terms of component diversity and amounts compared to other species. Especially the rosmarinic acid (34638.45 µg analyte/g extract) content of the leaf part of this species is remarkable. In general, antioxidant activities of all species were found to be high or moderate, while enzyme activities were low. This is the first detailed study in the literature on the chemical contents and biological activities of extracts of various parts of 5 endemic species prepared using different solvents (petroleum ether, chloroform and ethanol). The ethanol extract of the leaf of S. cerino-pruinosa var. elazigensis has high total phenolic, rosmarinic acid content and antioxidant potential and the cytotoxic activity of S. rosifolia has potential for use in food supplement, food preservative and pharmaceutical industries.

Keywords

Kaynakça

Akdeniz, M., Yener, I., Dincel, D., Firat, M., Karatas Degirmenci, D., & Ertas, A. (2022). Determination of fingerprints contents of different extracts and parts of six endemic Salvia taxa by GC–MS: Source species for valuable compounds with drug or drug potential. Biomedical Chromatography, 36, e5263. https://doi.org/10.1002/bmc.5263
Apak, R., Guclu, K., Ozyurek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981. https://doi.org/ 10.1021/jf048741x.
Bakir, D., Akdeniz, M., Ertas, A., Yilmaz, M. A., Yener, I., Firat, M., & Kolak, U. (2020). A GC-MS method validation for quantitative investigation of some chemical markers in Salvia hypargeia Fisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol. Journal of Food Biochemistry, 44, e13350. https://doi.org/ 10.1111/jfbc.13350.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
Cavusoğlu, M., Yigitkan, S., Yener, İ., Çağlayan, M. V., Reşitoğlu, B., Akdeniz, M., Çavuş Kaya, E., Tekin, F., Yılmaz, M. A., & Ertaş, A. (2024). Ziziphora clinopodioides Lam. türünün kültür ile doğal ortamlarda yetişen örneklerinin kimyasal ve biyolojik yönden detaylı incelenmesi. KSÜ Tarım ve Doğa Dergisi, 27(Suppl 2), 316–326. https://doi.org/10.18016/ksutarimdoga.vi.1585656
Delamare, A. P. L., Moschen-Pistorello, I. T., Artico, L., Atti-Serafini, L., & Echeverrigaray, S. (2007). Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chemistry, 100, 603-608. https://doi.org/10.1016/j.foodchem.2005.09.078
Demirezer, L. O., Gurbuz, P., Kelicen-Ugur, E. P., Bodur, M., Ozenver, N., Uz, A., & Guvenalp, Z. (2015). Molecular docking and ex vivo and in vitro anticholinesterase activity studies of Salvia sp. and highlighted rosmarinic acid. Turkish Journal of Medical Sciences, 45, 1141-1148. https://doi:10.3906/sag-1404-42
Demirezer, L. O., Kuruüzüm-Uz, A., Güvenalp, Z., & Kazaz, C. (2015). Phenolic compounds from Salvia species and their tyrosinase inhibitory activities. Journal of Enzyme Inhibition and Medicinal Chemistry, 30, 224–229. https://doi.org/10.3109/14756366.2014.917682

Dubois, M., Bailly, F., Mbemba, G., Mouscadet, J. F., Debyser, Z., Witvrouw, M., & Cotelle, P. (2008). Reaction of rosmarinic acid with nitrite ions in acidic conditions: discovery of nitro- and in vitro rosmarinic acids as new anti-HIV-1 agents. Journal of Medicinal Chemistry, 51, 2575-2579. https://doi.org/10.1021/jm7011134
Duru, M. E., Tel, G., Ozturk, M., & Harmandar, M. (2012). Chemical composition, antioxidant and anticholinesterase activities of the essential oil of Salvia chrysophylla Staph. Records of Natural Products, 6, 175-179.
Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7, 88-95. https://doi.org/ 10.1016/0006-2952(61)90145-9.
Ertas, A., Akdeniz, M., Yener, I., Ozturk, M., Tokul-Olmez, O., Firat, M., & Kolak, U. (2022). Essential oil, aroma, and fatty acid profiles of five endemic Salvia taxa from Turkey with chemometric analysis. Chemistry & Biodiversity, 19, e202100408. https://doi.org/10.1002/cbdv.202100408
Halfon, B., Cetin, O., Kokdil, G., & Topcu, G. (2019). Chemical investigation and bioactivity screening of Salvia cassia extracts. Records of Natural Products, 13, 156-166. http://doi.org/10.25135/rnp.99.18.05.291
Hearing, V.J., & Jiménez, M. (1987). Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. International Journal of Biochemistry, 19, 1141-7. https://doi.org/10.1016/0020-711X(87)90095-4
Hina, Z., Ghazala, H. R., Arfa K., Huma, S., Sabiha, T., & Ajmal, K. (2015). Anti-urease activity of Mimusops elengi Linn (Sapotaceae). European Journal of Medicinal Plants, 6, 223-230. https://doi.org/10.9734/ EJMP/2015/12240.
Kahraman, A., Dogan, M., & Celep, F. (2011). Salvia siirtica sp. nov. (Lamiaceae) from Turkey. Nordic Journal of Botany, 29, 397-401. https://doi.org/10.1111/j.1756-1051.2011.00916.x
Kolak, U. (2007). New diterpenoids from the aerial parts of Salvia pilifera. Turkish Journal of Chemistry, 31, 363-369. https://journals.tubitak.gov.tr/chem/vol31/iss3/12
Kosar, M., Goger, F., & Baser, K. H. C. (2008). In vitro antioxidant properties and phenolic composition of Salvia virgate Jacq. from Turkey. Journal of Agricultural and Food Chemistry, 569, 2369–2374. https://doi.org/10.1021/jf073516b
Kraunsoe, J. A. E., Claridge, T. D. W., & Lowe, G. (1996). Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry, 35, 9090-9096. https://doi.org/ 10.1021/bi953013b.
Miller, H. E. (1971). A simplified method for the evaluation of antioxidants. Journal of the American Oil Chemists Society, 48, 91. https://doi.org/10.1007/BF02635693
Mojarraba, M., Langzian, M. S., Emamic, S. A., Asilic, J., & Tayarani-Najaranb, Z. (2013). In vitro anti-proliferative and apoptotic activity of different fractions of Artemisia armeniaca. Revista Brasileira de Farmacognosia, 23, 783-7888. http://dx.doi.org/10.1590/S0102-695X2013000500010.
Moreno, M. I. N., Isla, M. I., Sampietro, A. R., & Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0.
Orhan, I., Kartal, M., Naz, Q., Ejaz, A., Yilmaz, G., Kan, Y., Konuklugil, B., Şener, B., & Choudhary, M. I. (2007). Antioxidant and anticholinesterase evaluation of selected Turkish Salvia species. Food Chemistry, 103, 1247-1254. https://doi.org/10.1016/j.foodchem.2006.10.030
Orhan, I. E., Senol, F. S., Gülpınar, A. R., Kartal, M., & Sener, B. (2010). Acetylcholinesterase and urease inhibitory activities of some Turkish Salvia species. Food Chemistry, 123, 709–715. https://doi.org/10.1016/ j.foodchem.2010.05.033
Ozer, H., Altun, A., Saraydin, S. U., Soylu, S., Goktas, S., Tuncer, E., Inan, D. S., Koksal, B., Temiz, T. K., Tepe, B., Sen, M., Karadayi, K., & Turan, M. (2013). Antitumoral effects of Salvia absconditiflora Greuter & Burdet syn. Salvia cryptantha Montbret & Aucher ex Benth. on breast cancer. Indian Journal of Traditional Knowledge, 12, 390-397.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Slinkard, K., & Singleton, V. L. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55.
Son, K. H., Oh, H. M., Choi, S. K., Han, D. C., & Kwon, B. M. (2005). Anti-tumor abietane diterpenes from the cones of Sequoia sempervirens. Bioorganic & Medicinal Chemistry Letters, 15, 2019-2021. https://doi.org/ 10.1016/j.bmcl.2005.02.057
Takeda, H., Tsuji, M., Inazu, M., Egashira, T., & Matsumiya, T. (2002). Rosmarinic acid and caffeic acid produce antidepressive-like effect in the forced swimming test in mice. European Journal of Pharmacology, 449, 261-267. https://doi.org/10.1016/S0014-2999(02)02037-X
Tayarani-Najaran, Z., Asili, J., Aioubi, E., & Emami, S. A. (2013). Growth inhibition and apoptosis induction of Salvia chloroleuca on MCF-7 breast cancer cell line. Iranian Journal of Pharmaceutical Sciences, 12, 789-799.
Thring, T. S. A., Hili, P., & Naughton, D.P. (2009). Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complementary and Alternative Medicine, 9, 27 (2009). https://doi.org/10.1186/1472-6882-9-27.
Topcu, G., Yucer, R., & Senol, H. (2017). Bioactive constituents of Anatolian Salvia species. In: Georgiev, V., Pavlov, A. (eds) Salvia Biotechnology. Springer, Cham. pp. https://doi.org/10.1007/978-3-319-73900-7_2
Uysal, S. (2019). A comparative study of three drying methods on the phenolic profile and biological activities of Salvia absconditiflora. Journal of Food Measurement and Characterization, 13, 162-168. https://doi.org/10.1007/s11694-018-9929-7
Yaris, E., Balur Adsız, L., Yener, I., Tuncay, E., Yilmaz, M. A., Akdeniz, M., Kaplaner, E., Firat, & M., Ertas, A., Kolak, U. (2021). Isolation of secondary metabolites of two endemic species: Salvia rosifolia Sm. and Salvia cerino-pruinosa Rech. f. var. elazigensis (Lamiaceae). Journal of Food Measurement and Characterization, 15, 4929-4938. https://doi.org/10.1007/s11694-021-01065-8
Yener, I., Ozhan Kocakaya, S., Ertas, A., Erhan, B., Kaplaner, E., Oral, E. V., Yilmaz-Ozden, T., & Yilmaz, M. A., Ozturk, M., Kolak, U. (2020). Selective in vitro and in silico enzymes inhibitory activities of phenolic acids and flavonoids of food plants: Relations with oxidative stress. Food Chemistry, 327, 127045. https://doi.org/10.1016/j.foodchem.2020.127045
Yigitkan, S., Akdeniz, M., Yener, İ., Firat, M., Senturk, K., Alkan, H., & Ertaş, A. (2021). Chemical composition and biological activity of Ixiolirion tataricum (Pall.) Schult. & Schult.f. var. tataricum. Records of Agricultural and Food Chemistry, 1(1–2), 27–34. https://doi.org/10.25135/rfac.5.2112.2301
Yiğitkan, S., Çavuşoğlu, M., Çağlayan, M. V., Yener, İ., Fırat, M., Çavuş Kaya, E., Yılmaz, M. A., & Ertaş, A. (2024). LC-MS/MS analysis and biological activities of different parts of Ziziphora capitata L. KSÜ Tarım ve Doğa Dergisi, 27(Suppl 2), 307–315. https://doi.org/10.18016/ksutarimdoga.vi.1585230
Yildirim, G. B., Gürer, Ç. Ü., Yener, I., Akdeniz, M., Yigitkan, S., Yılmaz, M. A., Tireki, S., Ertaş, A., & Demirkoz, A. B. (2025). Comparative evaluation of antioxidant and enzyme inhibition activities of pomegranate vinegars and extracts. Records of Agricultural and Food Chemistry, 37, 1–10. https://doi.org/10.25135/rfac.37.2510.3654
Yilmaz, M. A., Ertas, A., Yener, I., Akdeniz, M., Cakir, O., Altun, M., Demirtas, I., Boga, M., & Temel, H. (2018). A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala. Journal of Pharmaceutical and Biomedical Analysis, 154, 413-424. https://doi.org/10.1016/j.jpba.2018.02.059
Zengin, G., Atasagun, B., Aumeeruddy, M. Z., Saleem, H., Mollica, A., Bahadori, M. B., & Mahomoodally, M. F. (2019). Phenolic profiling and in vitro biological properties of two Lamiaceae species (Salvia modesta and Thymus argaeus): A comprehensive evaluation. Industrial Crops and Products, 128, 308-314. https://doi.org/10.1016/j.indcrop.2018.11.027
Zengin, G., Uysal, A., Gunes, E., & Aktumsek, A. (2019). Survey of phytochemical composition and biological effects of Salvia species: Tyrosinase and antioxidant perspectives. Industrial Crops and Products, 128, 365–373. https://doi.org/10.1016/j.indcrop.2018.11.034
Zengin, G., Aktumsek, A., Ceylan, R., & Picot-Allain, M. C. N. (2018). Enzyme inhibitory properties of different extracts obtained from Salvia species: Insights into urease and cholinesterase inhibition. Journal of Enzyme Inhibition and Medicinal Chemistry, 33, 1564–1572. https://doi.org/10.1080/14756366.2018.1514373
Zengin, G., Mahomoodally, M. F., Sinan, K. I., & Ak, G. (2020). Chemical composition and biological properties of Salvia species: Focus on elastase and collagenase inhibition. Phytochemistry Reviews, 19, 1315–1338. https://doi.org/10.1007/s11101-020-09670-4

Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Serkan Yiğitkan
0000-0002-6202-1515
Türkiye

Leyla Balur Adsız
0009-0001-9276-6892
Türkiye

Mustafa Abdullah Yılmaz
0000-0002-4090-7227
Türkiye

Evin Aygün
0009-0004-9680-6654
Türkiye

Hilal Saruhan Fidan
0000-0002-5790-9915
Türkiye

Esra Yariş
0009-0003-8575-0520
Türkiye

Mehmet Akdeniz
0000-0002-4435-4826
Türkiye

Mehmet Veysi Çağlayan
0000-0002-9803-6444
Türkiye

İsmail Yener
0000-0002-0988-9462
Türkiye

Mehmet Boğa
0000-0003-4163-9962
Türkiye

Mehmet Fırat
0000-0001-5814-614X
Türkiye

Ufuk Kolak
0000-0002-0339-635X
Türkiye

Abdulselam Ertaş ^*
0000-0002-2193-8386
Türkiye

Erken Görünüm Tarihi

27 Şubat 2026

Yayımlanma Tarihi

27 Şubat 2026

Gönderilme Tarihi

20 Ekim 2025

Kabul Tarihi

29 Aralık 2025

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1804808

IZ

https://izlik.org/JA69CL44RE

Kaynak Göster

RIS / Bibtex

APA

Yiğitkan, S., Balur Adsız, L., Yılmaz, M. A., Aygün, E., Saruhan Fidan, H., Yariş, E., Akdeniz, M., Çağlayan, M. V., Yener, İ., Boğa, M., Fırat, M., Kolak, U., & Ertaş, A. (2026). Chemical and biological evaluation of five endemic Salvia species: Rich source of rosmarinic, caffeic, fumaric and malic acids. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication, 849-862. https://doi.org/10.18016/ksutarimdoga.vi.1804808

Beş endemik Salvia türünün kimyasal ve biyolojik değerlendirmesi: Rosmarinik, kafeik, fumarik ve malik asitlerin zengin kaynağı

Öz

Anahtar Kelimeler

Chemical and biological evaluation of five endemic Salvia species: Rich source of rosmarinic, caffeic, fumaric and malic acids

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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