Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)

Mehmet Fidan

doi:10.18016/ksutarimdoga.vi.1872695

TR EN

Pirinç Kepeği ile Desteklenmiş Diyetin Drosophila melanogaster Meigen (Diptera: Drosophilidae)'de Yaşam Süresi ve Fonksiyonel Yaşlanma Üzerindeki Etkileri

Öz

Yaşlanma, azalan fizyolojik fonksiyonlar ve zayıflayan antioksidan savunma sistemleri ile karakterizedir. Biyoaktif bileşikler açısından zengin, besin değeri yüksek bir yan ürün olan pirinç kepeği, bu sistemleri modüle etme potansiyeline sahiptir, ancak yaşlanma üzerindeki in vivo etkileri henüz yeterince araştırılmamıştır. Bu çalışmada, pirinç kepeği ile desteklenmiş diyetin Drosophila melanogaster Meigen’de yaşam süresi, antioksidan enzim aktivitesi ve fonksiyonel kapasite üzerindeki etkisi araştırılmıştır. Yetişkin erkek sinekler kontrol veya pirinç kepeği ile desteklenmiş diyetlerle (%0.5, %1.0 veya %2.5 a/a) beslenmiştir. Hayatta kalma, süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPx) aktiviteleri ile lokomotor fonksiyon değerlendirilmiştir. Optimal pirinç kepeği dozajı, ortalama yaşam süresini %18-22 oranında ve median sağkalımı anlamlı şekilde uzatmıştır (p < 0.01); buna antioksidan enzim aktivitelerindeki artış eşlik etmiştir: SOD (%25-30), CAT (%20-25) ve GPx (%15-20) (p < 0.05). Desteklenmiş sinekler, kontrollere kıyasla azalmış lokomotor gerileme ve korunmuş tırmanma yeteneği sergilemişlerdir (p < 0.05). Bu bulgular, pirinç kepeği ile desteklenmiş diyetin, dengeli antioksidan regülasyonu aracılığıyla D. melanogaster'da ömür uzunluğunu ve fonksiyonel sürdürülebilirliği desteklediğini göstermekte ve optimize edilmiş redoks homeostazının sağlıklı yaşam süresinin kritik bir belirleyicisi olduğunu desteklemektedir.

Anahtar Kelimeler

Etik Beyan

Geçerli değildir. Bu çalışmada, etik kurul onayı gerektirmeyen bir omurgasız model organizma olan Drosophila melanogaster kullanılmıştır.

Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)

Öz

Aging is characterized by declining physiological functions and weakened antioxidant defenses. Rice bran, a nutrient-dense byproduct rich in bioactive compounds, offers potential to modulate these systems, yet its in vivo effects on aging remain underexplored. We investigated the impact of dietary rice bran supplementation on lifespan, antioxidant enzyme activity, and functional capacity in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Adult male flies were fed control or rice bran-supplemented diets (0.5%, 1.0%, or 2.5% w/w). Survival, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and locomotor function were assessed. Optimal rice bran dosage significantly extended mean lifespan by 18–22% and median survival (p < 0.01), accompanied by increased antioxidant enzyme activities: SOD (25–30%), CAT (20–25%), and GPx (15–20%) (p < 0.05). Supplemented flies exhibited attenuated locomotor decline and preserved climbing ability compared to controls (p < 0.05). These findings demonstrate that dietary rice bran promotes longevity and functional maintenance in D. melanogaster through balanced antioxidant regulation, supporting optimized redox homeostasis as a critical determinant of healthspan.

Anahtar Kelimeler

Etik Beyan

Not applicable. This study used Drosophila melanogaster, an invertebrate model organism that does not require ethics committee approval.

Kaynakça

Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Araujo, S.M., de Paula, M.T., Poetini, M.R., Meichtry, L., Bortolotto, V.C., Zarzecki, M.S., Jesse, C.R., & Prigol, M. (2015). Effectiveness of γ-oryzanol in reducing neuromotor deficits, dopamine depletion and oxidative stress in a Drosophila melanogaster model of Parkinson’s disease induced by rotenone. NeuroToxicology, 51, 96–105. https://doi.org/10.1016/j.neuro.2015.09.003
Ayar, A., Çolak, D.A., Uysal, H., & Fidan, M. (2021). Lifespan in Drosophila melanogaster feeding with white tea. Erzincan University Journal of Science and Technology, 14(2), 582–594. https://doi.org/10.18185/ erzifbed. 876718
Bak, N.K., Rohde, P.D., & Kristensen, T.N. (2023). Strong sex-dependent effects of malnutrition on life- and healthspan in Drosophila melanogaster. Insects, 14(1), 62. https://doi.org/10.3390/insects15010009
Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1), 276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Beghelli, D., Giusti, L., Zallocco, L., Ronci, M., Cappelli, A., Pontifex, M.G., Muller, M., Damiani, C., Cirilli, I., Hrelia, S., Vauzour, D., Vittadini, E., Favia, G., & Angeloni, C. (2024). Dietary fiber supplementation increases Drosophila melanogaster lifespan and gut microbiota diversity. Food & Function, 15(14), 7468–7477. https://doi.org/10.1039/d4fo00879k
Berry, R., III, & López-Martínez, G. (2020). A dose of experimental hormesis: When mild stress protects and improves animal performance. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 242, 110658. https://doi.org/10.1016/j.cbpa.2020.110658
Beyer, W., & Fridovich, I. (1987). Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical Biochemistry, 161(2), 559–566. https://doi.org/10.1016/0003-2697(87)90489-1

Calabrese, E.J., Osakabe, N., Di Paola, R., Siracusa, R., Fusco, R., D'Amico, R., Impellizzeri, D., Cuzzocrea, S., Fritsch, T., Abdelhameed, A.S., Wenzel, U., Franceschi, C., & Calabrese, V. (2023). Hormesis defines the limits of lifespan. Ageing Research Reviews, 91, 102074. https://doi.org/10.1016/j.arr.2023.102074
Colombo, R., Moretto, G., Barberis, M., Frosi, I., & Papetti, A. (2023). Rice byproduct compounds: From green extraction to antioxidant properties. Antioxidants, 12(1), 1–33. https://doi.org/10.3390/antiox12010100
Çolak, D.A., Ayar, A., & Uysal, H. (2014). The effects of Punica granatum L. ethanol extract including the antioxidant flavonoids on Drosophila melanogaster lifespan. Journal of Applied Biological Sciences, 8(3), 6–9.
Culletta, G., Buttari, B., Arese, M., Brogi, S., Almerico, A.M., Saso, L., & Tutone, M. (2024). Natural products as non-covalent and covalent modulators of the KEAP1/NRF2 pathway exerting antioxidant effects. European Journal of Medicinal Chemistry, 270, 116355. https://doi.org/10.1016/j.ejmech.2024.116355
Dai, X., Zhang, Q., Zhang, G., Ma, C., & Zhang, R. (2023). Protective effect of agar oligosaccharide on male Drosophila melanogaster suffering from oxidative stress via intestinal microflora activating the Keap1-Nrf2 signaling pathway. Carbohydrate Polymers, 313, 120878. https://doi.org/10.1016/j.carbpol.2023.120878
Das, P.P., Gul, M.Z., Weber, A.M., Srivastava, R.K., Marathi, B., Ryan, E.P., & Ghazi, I.A. (2024). Rice bran extraction and stabilization methods for nutrient and phytochemical biofortification, nutraceutical development, and dietary supplementation. Nutrition Reviews, 83(4), 692–712. https://doi.org/10.1093/ nutrit/nuae174
Duangjan, C., Rangsinth, P., Gu, X., Wink, M., & Tencomnao, T. (2019). Lifespan extending and oxidative stress resistance properties of a leaf extracts from Anacardium occidentale L. in Caenorhabditis elegans. Oxidative Medicine and Cellular Longevity, 2019, 9012396. https://doi.org/10.1155/2019/9012396
Fidan, M. (2025). Yaşam süresi ve üreme arasındaki dengede fitokimyasal modülasyon: Cistus creticus örneği. Tarım ve Doğa Dergisi, 28(5), 1214. http://doi.org/10.18016/ksutarimdoga.vi.1666340
Gargano, J.W., Martin, I., Bhandari, P., & Grotewiel, M. (2005). Rapid iterative negative geotaxis (RING): A new method for assessing age-related locomotor decline in Drosophila. Experimental Gerontology, 40(5), 386–395. https://doi.org/10.1016/j.exger.2005.02.005
Gong, X., Sui, L., Morton, J., Brennan, M., & Brennan, C. (2021). Investigation of nutritional and functional effects of rice bran protein hydrolysates by using Preferred Reporting Items for Systematic Reviews and Meta- Analysis (PRISMA) guidelines: A review. Trends in Food Science & Technology, 111, 594–607. https://doi.org/10.1016/ j.tifs.2021.01.089
Goufo, P., & Trindade, H. (2014). Rice antioxidants: Phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid. Food Science & Nutrition, 2(2), 75–104. https://doi.org/ 10.1002/fsn3.86
Grotewiel, M.S., Martin, I., Bhandari, P., & Cook-Wiens, E. (2005). Functional senescence in Drosophila melanogaster. Ageing Research Reviews, 4(3), 372–397. https://doi.org/10.1016/j.arr.2005.04.001
Guo, J., Huang, X., Dou, L., Yan, M., Shen, T., Tang, W., & Li, J. (2022). Aging and aging-related diseases: From molecular mechanisms to interventions and treatments. Signal Transduction and Targeted Therapy, 7(1), 391. https://doi.org/10.1038/s41392-022-01251-0
Güneş, E. (2016). Drosophila melanogaster Meigen (Diptera: Drosophilidae)’de kinoa (Chenopodium quinoa Willd.)’nın total oksidatif stres üzerinde etkisi. KSÜ Doğa Bilimleri Dergisi, 19(3), 261–267. https://izlik.org/ JA95JE24NE
Güneş, E., & Biçer, Ş.B. (2021). The effect of feeding with pumpkin seed membrane on survival, development and longevity in model organism. KSU Journal of Agriculture and Nature, 24(6), 1327–1332. https://doi.org/ 10.18016/ksutarimdoga.vi.839884
Güneş, E., & Nizamlıoğlu, H.F. (2023). The antioxidant effect of chitosan on virgin and mated Drosophila females. Carbohydrate Polymer Technologies and Applications, 5, 100297. https://doi.org/10.1016/j.carpta.2023.100297
Hafeez, E., Du, D., Ni, H., Zhu, K., Hu, F., Zhou, J., & Chen, D. (2025). Regulation and mechanism of Bletilla striata polysaccharide on delaying aging in Drosophila melanogaster. International Journal of Biological Macromolecules, 310, 143382. https://doi.org/10.1016/j.ijbiomac.2025.143382
Jaafaru, M. S., Muhammad, S. A., Mohammed, Z. K., Aliyu, Y., & Razis, A. F. A. (2024). Proanthocyanidins supplemented diet alter anti-aging-markers and improved lifespan in Drosophila melanogaster model. Beni-Suef University Journal of Basic and Applied Sciences, 13, 11. https://doi.org/10.1186/s43088-024-00469-x
Jena, A., Samal, R., Bhol, N., & Duttaroy, A. (2023). Cellular red-ox system in health and disease: The latest update. Biomedicine & Pharmacotherapy, 162, 114606. https://doi.org/10.1016/j.biopha.2023.114606
Jomová, K., Alomar, S.Y., Alwasel, S.H., Nepovimova, E., Kuca, K., & Valko, M. (2024). Several lines of antioxidant defense against oxidative stress: Antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants. Archives of Toxicology, 98(5), 1323–1367. https://doi.org/ 10.1007/s00204-024-03696-4
Jones, M.A., & Grotewiel, M. (2011). Drosophila as a model for age-related impairment in locomotor and other behaviors. Experimental Gerontology, 46(5), 320–325. https://doi.org/10.1016/j.exger.2010.08.012
Khan, S., Singer, B., & Vaughan, D. (2017). Molecular and physiological manifestations and measurement of aging in humans. Aging Cell, 16, 624–633. https://doi.org/10.1111/acel.12601
Kim, S.H., Kim, B.K., Park, S., & Park, S.K. (2019). Phosphatidylcholine extends lifespan via DAF-16 and reduces amyloid-beta-induced toxicity in Caenorhabditis elegans. Oxidative Medicine and Cellular Longevity, 2019, 2860642. https://doi.org/10.1155/2019/2860642
Landis, G.N., Doherty, D.V., & Tower, J. (2020). Analysis of Drosophila melanogaster lifespan. Methods in Molecular Biology, 2144, 47–56. https://doi.org/10.1007/978-1-0716-0592-9_4
Lee, J.-H., Lee, H.-Y., Cho, J.-C., Moskalev, A., & Min, K.-J. (2025). Microbiota-dependent longevity effects of Aronia berry (Aronia melanocarpa) in Drosophila melanogaster. Food Frontiers, 6(5), 2328–2344. https://doi.org/10.1002/fft2.70078
Li, Y., Tian, X., Luo, J., Bao, T., Wang, S., & Wu, X. (2024). Molecular mechanisms of aging and anti-aging strategies. Cell Communication and Signaling, 22(1), 285. https://doi.org/10.1186/s12964-024-01663-1
Li, Y., Zhang, W., Ye, Y., Sun, Y., Yang, L., Chen, G., Chen, K., Smith, S., & Zhou, J. (2023). Atg4b overexpression extends lifespan and healthspan in Drosophila melanogaster. International Journal of Molecular Sciences, 24(12), 9893. https://doi.org/10.3390/ijms24129893
Linford, N.J., Bilgir, C., Ro, J., & Pletcher, S.D. (2013). Measurement of lifespan in Drosophila melanogaster. Journal of Visualized Experiments, (71), 50068. https://doi.org/10.3791/50068
Liu, S., Pi, J., & Zhang, Q. (2022). Signal amplification in the KEAP1-NRF2-ARE antioxidant response pathway. Redox Biology, 54, 102389. https://doi.org/10.1016/j.redox.2022.102389
Lowry, O., Rosebrough, N., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.
Ma, C., Yang, K., Wang, Y., & Dai, X. (2019). Anti-aging effect of agar oligosaccharide on male Drosophila melanogaster and its preliminary mechanism. Marine Drugs, 17(11), 632. https://doi.org/10.3390/md17110632
Mahfudh, N., Rahmawati, I., Maulana, M.F., Sulistyani, N., & Kumalasari, I.D. (2025). Antioxidant activities of soybean (Glycine max L.), sweet potato leaves (Ipomoea batatas L.) and red yeast rice (Monascus purpureus) functional drink in managing hyperlipidemia. Food Research, 9(2), 299–305. https://doi.org/10.26656/ fr.2017.9(2).229
Moretti, C.H., Schiffer, T.A., Montenegro, M.F., Larsen, F.J., Tsarouhas, V., Carlström, M., Samakovlis, C., Weitzberg, E., & Lundberg, J.O. (2020). Dietary nitrite extends lifespan and prevents age-related locomotor decline in the fruit fly. Free Radical Biology and Medicine, 160, 860–870. https://doi.org/10.1016/ j.freeradbiomed.2020.09.018
Nichols, C.D., Becnel, J., & Pandey, U.B. (2012). Methods to assay Drosophila behavior. Journal of Visualized Experiments, (61), e3795. https://doi.org/10.3791/3795
Obeme-Nmom, J.I., Abioye, R.O., Reyes Flores, S.S., & Udenigwe, C.C. (2024). Regulation of redox enzymes by nutraceuticals: a review of the roles of antioxidant polyphenols and peptides. Food & Function, 15, 10956–10980. https://doi.org/10.1039/d4fo03549f
Paglia, D.E., & Valentine, W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine, 70(1), 158–169.
Park, H., Lee, K., & Choi, H. (2017). Rice bran constituents: immunomodulatory and therapeutic activities. Food & Function, 8(3), 935–943. https://doi.org/10.1039/c6fo01763k
Partridge, L., Gems, D., & Withers, D.J. (2005). Sex and death: what is the connection? Cell, 120(4), 461–472. https://doi.org/10.1016/j.cell.2005.01.026
Pfluger, B.A., Smith, H.V., Weber, A.M., Ibrahim, H., Doumbia, L., Bore, A., Cissoko, A., Douyon, S., Kone, K., Sangare, L., Maiga, A., Koita, O.A., Goodman, K., Evans, A.M., & Ryan, E.P. (2022). Non-targeted dried blood spot-based metabolomics analysis showed rice bran supplementation effects multiple metabolic pathways during infant weaning and growth in Mali. Nutrients, 14(3), 609. https://doi.org/10.3390/nu14030609
Phongthai, S., D’Amico, S., Schoenlechner, R., Homthawornchoo, W., & Rawdkuen, S. (2018). Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion. Food Chemistry, 240, 156–164. https://doi.org/10.1016/j.foodchem.2017.07.080
Piper, M.D., & Partridge, L. (2016). Protocols to study aging in Drosophila. Drosophila: Methods and Protocols, 291–302. https://doi.org/10.1007/978-1-4939-6371-3_18
Piper, M.D.W., & Partridge, L. (2007). Dietary restriction in Drosophila: delayed aging or experimental artefact? PLOS Genetics, 3(4), e57. https://doi.org/10.1371/journal.pgen.0030057
Płóciniczak, A., Bukowska-Olech, E., & Wysocka, E. (2025). The complexity of oxidative stress in human age-related diseases: A review. Metabolites, 15(7), 479. https://doi.org/10.3390/metabo15070479
R Core Team. (2023). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.R-project.org/
Raghunath, A., Sundarraj, K., Nagarajan, R., Arfuso, F., Bian, J., Kumar, A.P., Sethi, G., & Perumal, E. (2018). Antioxidant response elements: Discovery, classes, regulation and potential applications. Redox Biology, 17, 297–314. https://doi.org/10.1016/j.redox.2018.05.002
Ramírez-Mendoza, A.A., Ramírez-Herrera, M.A., Cortez-Álvarez, C.R., Nery-Flores, S.D., Tejeda-Martínez, A.R., Romero-Prado, M.M.d.J., & Mendoza-Magaña, M.L. (2022). Curcumin modifies the activity of plasmatic antioxidant enzymes and the hippocampal oxidative profile in rats upon acute and chronic exposure to ozone. Molecules, 27(14), 4531. https://doi.org/10.3390/molecules27144531
Rana, A., Oliveira, M.P., Khamoui, A.V., Aparicio, R., Rera, M., Rossiter, H.B., & Walker, D.W. (2017). Promoting Drp1-mediated mitochondrial fission in midlife prolongs healthy lifespan of Drosophila melanogaster. Nature Communications, 8(1), 448. https://doi.org/10.1038/s41467-017-00525-4
Ren, L., Fan, J., Yang, Y., Xu, Y., Chen, F., Bian, X., Xing, T., Liu, L., Yu, D., & Zhang, N. (2021). Enzymatic hydrolysis of broken rice protein: Antioxidant activities by chemical and cellular antioxidant methods. Frontiers in Nutrition, 8, 788078. https://doi.org/10.3389/fnut.2021.788078
Rhodenizer, D., Martin, I., Bhandari, P., Pletcher, S.D., & Grotewiel, M. (2008). Genetic and environmental factors impact age-related impairment of negative geotaxis in Drosophila by altering age-dependent climbing speed. Experimental Gerontology, 43(8), 739–748. https://doi.org/10.1016/j.exger.2008.04.011
Rungratanawanich, W., Abate, G., Serafini, M.M., Guarienti, M., Catanzaro, M., Marziano, M., Memo, M., Lanni, C., & Uberti, D. (2018). Characterization of the antioxidant effects of γ-oryzanol: Involvement of the Nrf2 pathway. Oxidative Medicine and Cellular Longevity, 2018, 2987249. https://doi.org/10.1155/2018/2987249
Saka, S.O., Salisu, Y.Y., Sahabi, H.M., Sanusi, K.O., Ibrahim, K.G., Abubakar, M.B., Isa, S.A., Liman, M.G., Shehu, S., Malami, I., Chan, K.W., Azmi, N.H., & Imam, M.U. (2023). Nutrigenomic effects of white rice and brown rice on the pathogenesis of metabolic disorders in a fruit fly model. Molecules, 28(2), 532. https://doi.org/10.3390/ molecules28020532
Sekiya, M., & Iijima, K.M. (2021). Phenotypic analysis of a transgenic Drosophila model of Alzheimer’s amyloid-β toxicity. STAR Protocols, 2(2), 100501. https://doi.org/10.1016/j.xpro.2021.100501
Sen, S., Chakraborty, R., & Kalita, P. (2020). Rice - not just a staple food: A comprehensive review on its phytochemical composition and therapeutic potential. Trends in Food Science & Technology, 97, 265–285. https://doi.org/10.1016/j.tifs.2020.01.022
Shaposhnikov, M.V., Guvatova, Z.G., Zemskaya, N.V., Koval, L.A., Schegoleva, E.V., Gorbunova, A.A., Golubev, D.A., Pakshina, N.R., Ulyasheva, N.S., Solovev, I.A., Bobrovskikh, M.A., Gruntenko, N.E., Menshanov, P.N., Krasnov, G.S., Kudryavseva, A.V., & Moskalev, A.A. (2022). Molecular mechanisms of exceptional lifespan increase of Drosophila melanogaster with different genotypes after combinations of pro-longevity interventions. Communications Biology, 5(1), 566. https://doi.org/10.1038/s42003-022-03524-4
Sohail, M., Rakha, A., Butt, M., Iqbal, M., & Rashid, S. (2017). Rice bran nutraceutics: A comprehensive review. Critical Reviews in Food Science and Nutrition, 57, 3771–3780. https://doi.org/10.1080/10408398.2016.1164120
Spaggiari, M., Dall’Asta, C., Galaverna, G., & Del Castillo Bilbao, M.D. (2021). Rice bran by-product: From valorization strategies to nutritional perspectives. Foods, 10(1), 85. https://doi.org/10.3390/foods10010085
Spiers, J.G., Breda, C., Robinson, S., Giorgini, F., & Steinert, J.R. (2019). Drosophila Nrf2/Keap1 mediated redox signaling supports synaptic function and longevity and impacts on circadian activity. Frontiers in Molecular Neuroscience, 12, 86. https://doi.org/10.3389/fnmol.2019.00086
Staats, S., Lürsen, K., Wagner, A.E., & Rimbach, G. (2018). Drosophila melanogaster as a versatile model organism in food and nutrition research. Journal of Agricultural and Food Chemistry, 66(15), 3737–3753. https://doi.org/10.1021/acs.jafc.7b05900
Sunthonkun, P., Palajai, R., Somboon, P., Suan, C.L., Ungsurangsri, M., & Soontorngun, N. (2019). Life-span extension by pigmented rice bran in the model yeast Saccharomyces cerevisiae. Scientific Reports, 9(1), 18061. https://doi.org/10.1038/s41598-019-54448-9
Sykiotis, G.P., & Bohmann, D. (2008). Keap1/Nrf2 signaling regulates oxidative stress tolerance and lifespan in Drosophila. Developmental Cell, 14(1), 76–85. https://doi.org/10.1016/j.devcel.2007.12.002
Tan, B. L., Norhaizan, M. E., Liew, W. P. P., & Rahman, H. S. (2018). Antioxidant and oxidative stress: A mutual interplay in age-related diseases. Frontiers in Pharmacology, 9, 1162. https://doi.org/10.3389/fphar.2018.01162
Tan, B.L., Norhaizan, M.E., & Chan, L.C. (2023). Rice bran: From waste to nutritious food ingredients. Nutrients, 15(11), 2503. https://doi.org/10.3390/nu15112503
Taylor, A.L., Dubuisson, O., Pandey, P., Zunica, E.R.M., Vandanmagsar, B., Dantas, W.S., Johnson, A., Axelrod, C.L., & Kirwan, J.P. (2024). Restricting bioenergetic efficiency enhances longevity and mitochondrial redox capacity in Drosophila melanogaster. Aging Cell, 23(5), e14107. https://doi.org/10.1111/acel.14107
Thiruvengadam, M., Venkidasamy, B., Subramanian, U., Samynathan, R., Ali Shariati, M., Rebezov, M., Girish, S., Thangavel, S., Dhanapal, A.R., Fedoseeva, N., Lee, J., & Chung, I.-M. (2021). Bioactive compounds in oxidative stress-mediated diseases: Targeting the NRF2/ARE signaling pathway and epigenetic regulation. Antioxidants, 10(12), 1859. https://doi.org/10.3390/antiox10121859
Uysal, H., Aydogan, M.N., & Algur, O.F. (2002). Effect of single cell protein as a protein source in Drosophila culture. Brazilian Journal of Microbiology, 33(4), 314–317. https://doi.org/10.1590/S1517-83822002000400007
Varela-López, A., Romero-Márquez, J.M., Navarro-Hortal, M.D., Ramirez-Tortosa, C.L., Battino, M., Forbes-Hernández, T.Y., & Quiles, J.L. (2023). Dietary antioxidants and lifespan: Relevance of environmental conditions, diet, and genotype of experimental models. Experimental Gerontology, 178, 112221. https://doi.org/10.1016/j.exger.2023.112221
Varesi, A., Chirumbolo, S., Campagnoli, L.I.M., Pierella, E., Piccini, G.B., Carrara, A., Ricevuti, G., Scassellati, C., Bonvicini, C., & Pascale, A. (2022). The role of antioxidants in the interplay between oxidative stress and senescence. Antioxidants, 11(7), 1224. https://doi.org/10.3390/antiox11071224
Vincent, A., Thauvin, M., Quévrain, E., Mathieu, E., Layani, S., Seksik, P., Batinic-Haberle, I., Vriz, S., Policar, C., & Delsuc, N. (2021). Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models. Journal of Inorganic Biochemistry, 219, 111431. https://doi.org/10.1016/ j.jinorgbio.2021.111431
Wang, H., Zhang, S., Zhai, L., Sun, L., Zhao, D., Wang, Z., & Li, X. (2021). Ginsenoside extract from ginseng extends lifespan and health span in Caenorhabditis elegans. Food & Function, 12(15), 6793–6808. https://doi.org/10.1039/d1fo00576f
Wattanasiritham, L., Theerakulkait, C., Wickramasekara, S., Maier, C.S., & Stevens, J.F. (2016). Isolation and identification of antioxidant peptides from enzymatically hydrolyzed rice bran protein. Food Chemistry, 192, 156–162. https://doi.org/10.1016/j.foodchem.2015.06.057
Weinrich, T.W., Coyne, A., Salt, T.E., Hogg, C., & Jeffery, G. (2017). Improving mitochondrial function significantly reduces metabolic, visual, motor and cognitive decline in aged Drosophila melanogaster. Neurobiology of Aging, 60, 34–43. https://doi.org/10.1016/j.neurobiolaging.2017.08.016
Xiao, J., Li, Y., Chen, B., Gong, S., Li, Z., Ou, Y., Ou, Z., Kang, A., Jia, Y., Yang, A., Cao, Y., & Miao, J. (2020). Enzymatic preparation and antioxidative activity of hydrolysate from rice bran protein. Journal of Food Measurement and Characterization, 14(6), 3163–3174. https://doi.org/10.1007/s11694-020-00563-5
Yue, Y., Wang, M., Feng, Z., Zhu, Y., & Chen, J. (2021). Antiaging effects of rice protein hydrolysates on Drosophila melanogaster. Journal of Food Biochemistry, e13602. https://doi.org/10.1111/jfbc.13602
Yusuf, A.O., Danborno, B., Bauchi, Z.M., Sani, D., & Ndams, I.S. (2024). Aging impaired locomotor and biochemical activities in Drosophila melanogaster Oregon R (fruit fly) model. Experimental Gerontology, 197, 112593. https://doi.org/10.1016/j.exger.2024.112593
Zaky, A.A., Chen, Z., Liu, Y., Li, S., & Jia, Y. (2019). Preparation and assessment of bioactive extracts having antioxidant activity from rice bran protein hydrolysates. Journal of Food Measurement and Characterization, 13(4), 2542–2548. https://doi.org/10.1007/s11694-019-00174-9
Zaky, A. A., Chen, Z., Qin, M., Wang, M., & Jia, Y. (2021). Assessment of antioxidant activity, amino acids, phenolic acids and functional attributes in defatted rice bran and rice bran protein concentrate. Progress in Nutrition, 22(4), e2020069. https://doi.org/10.23751/pn.v22i4.8971
Zambrana, L.E., McKeen, S., Ibrahim, H., Zarei, I., Borresen, E.C., Doumbia, L., Boré, A., Cissoko, A., Douyon, S., Koné, K., Perez, J., Perez, C., Hess, A., Abdo, Z., Sangaré, L., Maiga, A., Becker-Dreps, S., Yuan, L., Koita, O., ... Ryan, E.P. (2019). Rice bran supplementation modulates growth, microbiota and metabolome in weaning infants: a clinical trial in Nicaragua and Mali. Scientific Reports, 9(1), 13919. https://doi.org/10.1038/s41598-019-50344-4
Zhou, Y., Jiang, Z., Lu, H., Xu, Z., Tong, R., Shi, J., & Jia, G. (2019). Recent advances of natural polyphenols activators for Keap1-Nrf2 signaling pathway. Chemistry & Biodiversity, 16(11), e1900400. https://doi.org/10.1002/cbdv.201900400

Ayrıntılar

Birincil Dil

İngilizce

Konular

Entomoloji

Bölüm

Araştırma Makalesi

Yazarlar

Mehmet Fidan ^*
0000-0001-9016-6730
Türkiye

Erken Görünüm Tarihi

20 Eylül 2026

Yayımlanma Tarihi

-

Gönderilme Tarihi

27 Ocak 2026

Kabul Tarihi

6 Mayıs 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1872695

IZ

https://izlik.org/JA48MG36CS

Kaynak Göster

RIS / Bibtex

APA

Fidan, M. (2026). Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication, 160-174. https://doi.org/10.18016/ksutarimdoga.vi.1872695

AMA

1.Fidan M. Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;(Advanced Online Publication):160-174. doi:10.18016/ksutarimdoga.vi.1872695

Chicago

Fidan, Mehmet. 2026. “Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication: 160-74. https://doi.org/10.18016/ksutarimdoga.vi.1872695.

EndNote

Fidan M (01 Eylül 2026) Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication 160–174.

IEEE

[1]M. Fidan, “Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)”, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, ss. 160–174, Eyl. 2026, doi: 10.18016/ksutarimdoga.vi.1872695.

ISNAD

Fidan, Mehmet. “Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (01 Eylül 2026): 160-174. https://doi.org/10.18016/ksutarimdoga.vi.1872695.

JAMA

1.Fidan M. Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;:160–174.

MLA

Fidan, Mehmet. “Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Eylül 2026, ss. 160-74, doi:10.18016/ksutarimdoga.vi.1872695.

Vancouver

1.Mehmet Fidan. Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 01 Eylül 2026;(Advanced Online Publication):160-74. doi:10.18016/ksutarimdoga.vi.1872695

Pirinç Kepeği ile Desteklenmiş Diyetin Drosophila melanogaster Meigen (Diptera: Drosophilidae)'de Yaşam Süresi ve Fonksiyonel Yaşlanma Üzerindeki Etkileri

Öz

Anahtar Kelimeler

Etik Beyan

Rice Bran as a Novel Dietary Supplement Improves Lifespan, Antioxidant Defense, and Locomotor Performance in Drosophila melanogaster Meigen (Diptera: Drosophilidae)

Öz

Anahtar Kelimeler

Etik Beyan

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149