Abelmoschus esculentus (Bamya) Çiçeğinin Fitokimyasal Profili, Antioksidan, Antikolinerjik ve Antibakteriyel Özellikleri

Arzu Kavaz Yüksel; Emrah Dikici; Mehmet Yüksel; Mesut Işık

doi:10.18016/ksutarimdoga.vi.976717

TR EN

Abelmoschus esculentus (Bamya) Çiçeğinin Fitokimyasal Profili, Antioksidan, Antikolinerjik ve Antibakteriyel Özellikleri

Öz

Bu araştırmanın amacı, Abelmoschus esculentus’a ait çiçek kısımınlarının etanolik ekstraktının antioksidan ve antikolinerjik özelliklerini, fenolik bileşik profilini ve antibakteriyel aktivitesini araştırmaktır. Fenolik bileşiklerin analizi LC-MS/MS ile gerçekleştirilmiştir. Antioksidan kapasitesi (radikal giderme, metal indirgeme gücü ve toplam antioksidan aktivite) DPPH, ABTS, Cu2+-Cu+ indirgeme (CUPRAC), Fe3+-Fe2+ indirgeme ve ferrik tiyosiyanat yöntemleri ile değerlendirilmiştir. Antibakteriyel aktivite, disk difüzyon ve MIC (Minimum inhibitör konsantrasyonu) yöntemleri ile belirlenmiştir. Antikolinerjik özellik ise, asetilkolinesterazın (AChE) inhibisyonu ile tespit edilmiştir. Bitki özütünde miktar bakımından en fazla bulunan başlıca fenolik bileşiğin asetohidroksamik asit olduğu belirlenmiştir. Ayrıca, sırasıyla kuarsetin, myrisetin, fumarik asit, vanillik asit, ellagik asit, 4-hidroksibenzoik asit, salisilik asit, kafeik asit, kemferol, bütein, protokateşik asit, kateşin hidrat, oleuropein ve diğer bileşenler tespit edilmiştir. Bitkinin etanolik özütü, %29.41 DPPH radikal giderme aktivitesi gösterirken, %20.59 ABTS radikal giderme aktivitesine ve ayrıca orta düzeyde metal indirgeme potansiyeline sahiptir. Ayrıca ekstrakt, 0.18 mg mL-1 IC50 değeri ile AChE üzerinde bir inhibisyon etkisi göstermiştir. Bitkinin etanol özütü, Staphylococcus aureus, Escherichia coli ve Salmonella Typhimurium üzerinde farklı düzeylerde antibakteriyel etki göstermiştir. Elde edilen sonuçlar, A. esculentus çiçek özütünün, sahip olduğu antioksidan, antikolinerjik ve antibakteriyel özellikleri ile bazı hastalıkların tedavisinde rol oynayabileceğini düşündürmektedir.

Anahtar Kelimeler

Phytochemical Profile, Antioxidant, Anticholinergic and Antibacterial Properties of Flowers of Abelmoschus Esculentus (Okra Flowers)

Abstract

The aim of this research was to investigate the antioxidant, and anticholinergic properties, phenolics profile and antibacterial activities of Abelmoschus esculentus ethanol extract. The analysis of phenolic compounds was performed with LC–MS/MS. The antioxidant capacity (radical scavenging, metal-reducing power and total antioxidant activity) was assessed by DPPH, ABTS, Cu2+–Cu+ reducing (CUPRAC), Fe3+–Fe2+ reducing and ferric thiocyanate methods. The antibacterial activity was determined by disc diffusion and MIC (Minimum inhibitory concentration) methods and the anticholinergic property was predicted by inhibition of acetylcholinesterase (AChE). Acetohydroxamic acid was determined as the main phenolic compound with the highest amount in the flower extract. Also, quercetin, myricetin, fumaric acid, vanillic acid, ellagic acid, 4-hydroxybenzoic acid, salicylic acid, caffeic acid, kaempferol, butein, protocatechic acid, catechin hydrate, oleuropein and other components were detected, respectively. The ethanolic extract of the plant has 29.41% DPPH radical scavenging activity, 20.59% ABTS radical scavenging activity, and also moderate metal reduction potential. Also, the extract showed an inhibition effect on the AChE with IC50 values (0.18 mg mL−1). The ethanol extract of the plant showed antibacterial effect on Staphylococcus aureus, Escherichia coli, and Salmonella Typhimurium at different levels. These results suggested that extract of the flowers of A. esculentus extract might play a role in the treatment of some diseases with its antioxidant, anticholinergic, and antibacterial activity.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Arzu Kavaz Yüksel
0000-0001-8292-9259
Türkiye

Emrah Dikici
0000-0002-3086-8156
Türkiye

Mehmet Yüksel ^*
0000-0001-6566-1385
Türkiye

Mesut Işık
0000-0002-4677-8104
Türkiye

Yayımlanma Tarihi

30 Aralık 2022

Gönderilme Tarihi

30 Temmuz 2021

Kabul Tarihi

26 Kasım 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.976717

IZ

https://izlik.org/JA43WY28DR

Kaynak Göster

RIS / Bibtex

APA

Kavaz Yüksel, A., Dikici, E., Yüksel, M., & Işık, M. (2022). Abelmoschus esculentus (Bamya) Çiçeğinin Fitokimyasal Profili, Antioksidan, Antikolinerjik ve Antibakteriyel Özellikleri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(6), 1205-1215. https://doi.org/10.18016/ksutarimdoga.vi.976717