Determination of Some Bioactive Components, Pigment Content and Antioxidant Capacity in Fresh and Dry Leaf Samples of Cistus creticus L. Grown in Alanya Ecological Conditions]
Öz
Cistus creticus L. is one of the important medicinal plants spreading along the northern, southern and western coasts of Turkey. It has been used in folk medicine since ancient times for its antimicrobial, anti-inflammatory, cytotoxic and antiulcerogenic properties. The fresh and dry leaves of the plant are generally consumed as tea. This study aims to investigate some biochemical properties of fresh and dried leaves of Cistus creticus L., which grows naturally in Alanya ecological conditions. For this purpose, in leaf samples harvested in the spring period when the vegetative development of the plant is highest; total ascorbic acid, total phenolic, total flavonoid, total chlorophyll and total carotenoid contents and antioxidant activity were investigated. Except for the total ascorbic acid content, all parameters were higher in dry leaf samples than in fresh leaf samples. It is thought that the consumption of the plant is important because it contains a high degree of bioactive component and has a radical scavenging activity of around 50%.
Anahtar Kelimeler
Cistus creticus Antioxidant capacity Carotenoid Chlorophyll Phenolic
Kaynakça
- Acıbuca V, Bostan Budak D 2018. Dünya’da ve Türkiye’de Tıbbi ve Aromatik Bitkilerin Yeri ve Önemi. Çukurova Tarım Gıda Bil. Der. 33(1): 37-44.
- Akkol EK., Orhan IE, Yesilada E 2012. Anticholinesterase and antioxidant Effects of The Ethanol Extract, Ethanol Fractions And Isolated Flavonoids from Cistus laurifolius L. Leaves. Food Chemistry. 131(2): 626-631.
- Amensour M, Sendra E, Pérez-Alvarez JA, Skali-Senhaji N, Abrini J, Fernandez Lopez J 2010. Antioxidant Activity and Chemical Content of Methanol and Ethanol Extracts From Leaves of Rockrose (Cistus ladaniferus). Plant Foods Hum Nutr. 65:170–178.
- Anonim 2021. Konya Gıda ve Tarım Üniversitesinden Yerli Pastil. https://www.dha.com.tr/ saglikyasam/ konya-gida-ve-tarim-universitesinden-yerli pastil/haber-1808546 (Alınma Tarihi: 04.11.2021)
- AOAC, 1990. Official Methods of Analysis of The Association of Official Analytical Chemists 15th ed., Arlington VA, USA, 1058-1059.
- Bakhshi D, Arakawa O 2006. Effects of UV-B Irradiation on Phenolic Compound Accumulation And Antioxidant Activity in ‘Jonathan’ Apple Influenced by Bagging, Temperature and Maturation. Journal of Food, Agriculture & Environment. 4(1): 75-79.
- Barrajón-Catalán E, Tomás-Menor L, Morales-Soto A, Bruňá NM, López DS, Segura-Carretero A, Micoli V 2016. Rockroses (Cistus sp.) Oils. Essential Oils in Food Prevention. Flavor Saf, 74: 649-657.
- Benzie IFF, Strain JJ 1996. The Ferric Reducing Ability of Plasma (FRAB) as a Measure of Antioxidant Power: The FRAB Assay, Analytical Biochemistry, 239: 70-76.
- Davis PH 1965. Flora of Turkey and the East Aegean Islands, I. Edinburgh: Edinburgh Univ Press.
- De-Kok L, Graham M 1980. Levels of Pigments, Soluble Proteins, Amino Acids and Sulfhydryl Compounds in Foliar Tissue of Arabidopsis thaliana During Dark İnduced And Natural Senescence. Plant Physiology and Biochemistry. 27: 133-142.
- Dimcheva V, Karsheva M 2017. Antioxidant Activity and Polyphenolic Content of The Bulgarian Wild Herb Cistus incanus Stored Under Different Conditions. J Chem Technol Metall 52(5):781-790.
- El Euch SK., Bouajila J, Bouzouita N 2015.Chemical Composition, Biological and Cytotoxic Activities of Cistus salviifolius Flower Buds and Leaves Extracts. Industrial Crops and Products 76:1100–1105.
- Piluzza G, Bullitta S 2011. Correlations Between Phenolic Content And Antioxidant Properties in Twenty-Four Plant Species of Traditional Ethnoveterinary Use in the Mediterranean Area. Pharm. Biol. 49 (3): 240–247 .
- Gawel-Beben K, Kukula-Koch W, Hoian U, Czop M, Strzepek-Gomolka, M, Antosiewicz B 2020. Characterization of Cistus incanus L. and Cistus ladanifer L. Extracts As Potential Multifunctional Antioxidant Ingredients For Skin Protecting Cosmetics. Antioxidants. 9(3): 202.
- Guimarães R, Barros L, Carvalho AM, Sousa MJ, Morais JS, Ferreira IC 2009. Aromatic Plants as a Source of Important Phytochemicals: Vitamins, Sugars and Fatty Acids in Cistus ladanifer, Cupressus lusitanica and Eucalyptus gunnii Leaves. Industrial crops and products. 30(3): 427-430.
- Guvenc A, Yildiz S, Ozkan AM, Erdurak CS, Coşkun M, Yılmaz G, Okuyama T, Okada Y 2005. Antimicrobiological Studies on Turkish Cistus Species. Pharmaceutical Biology, 43(2): 178-183.
- Joshi APK., Rupasinghe HPV, Khanizadeh S 2011. Impact of Drying Processes on Bioactive Phenolics, Vitamin C and Antioxidant Capacity of Red‐Fleshed Apple Slices. Journal of Food Processing and Preservation. 35(4): 453-457.
- Kamiloglu S, Capanoglu E 2015. Polyphenol Content in Figs (Ficus carica L.): Effect of Sun-drying. International Journal of Food Properties, 18(3): 521-535.
- Kilic DD, Siriken B, Erturk O, Tanrikulu G, Melek Gul, Başkan C 2019. Antibacterial, Antioxidant and DNA Interaction Properties of Cistus creticus L. Extracts. Journal of International Environmental Application and Science, 14(3): 110-115.
- Kose MD, Tekin BN, Bayraktar O, Duman ET, Başpınar Y 2017. Antioxidant and Antimicrobial Properties of Cistus ladanifer. International Journal of Secondary Metabolite, 4(3, Special Issue 2): 434-444.
- Kumar SS, Manoj P, Shetty NP, Giridhar P 2015. Effect of Different Drying Methods on Chlorophyll, Ascorbic Acid and Antioxidant Compounds Retention of Leaves of Hibiscus sabdariffa L. Journal of the Science of Food and Agriculture. 95(9):1812-1820.
- Lahcen SA, El Hattabi L, Benkaddour R, Chahboun N, Ghanmi M, Satrani B, Zarrouk A 2020. Chemical Composition, Antioxidant, Antimicrobial and Antifungal Activity of Moroccan Cistus creticus Leaves. Chemical Data Collections. 26: 100346.
- Lanfer-Marquez UM, Barros RMC, Sinnecker P 2005. Antioxidant activity of chlorophylls and their derivatives. Food Research International 38(8-9): 885-891.
- Lichtenthaler K, Welburn AR 1983. Determination of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents. Botanisches Institutder Univeristat, Kaiserstran βe 12, Postfach 591-592.
- Liu X, Wang H, Chen Z 2016. Effect of Carotenoids on Body Colour of Discus Fish (Symphysodon aequifasciatus axelrodi Schultz, 1960). Aquaculture Research. 47(4): 130.
- Murathan ZT, Kaya A 2020. Alanya Ekolojik Koşullarında Yetiştirilen Hass ve Fuerte Avokado Çeşitlerinin Bazı Fitokimyasal İçerikleri ile Antioksidan Aktivitelerinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 23(6): 1435-1440.
- Nindo C, Sun T, Wang SW, Tang J , Powers JR 2003. Evaluation of Drying Technologies for Retention of Physical Quality and Antioxidants in Asparagus (Asparagus officinalis, L.). LWT-Food Science and Technology. 36(5): 507-516.
- Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet J, Luyck M, Cazin M, Cazin J.C, Bailleul F, Trotin F 2000. Phenolic Compounds and Antioxidant Activities of Buckwheat (Fagopyrum esculentum Moench) Hulls and Flour. Journal of Ethnopharmacology. 72: 35-40.
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26(9-10): 1231- 1237.
- Riehle P, Rohn S 2013. Phenolic Compounds in Cistus incanus Herbal Infusion-Antioxidant Capacity and Thermal Stability During The Brewing Process. Food Research International. 53(2): 891-899.
- Sekeroglu N, Gezici S 2021. Rockrose (Cistus spp.) Species as Turkey’s Virus Repellent Plants: Traditional Uses, Bioactive Chemical Components and Pharmacological Activities. Lokman Hekim Dergisi. 11(2):258-268.
- Soysal Y, Oztekin S 1998. Mentha piperita (Tibbi Nane)'de Kurutma Havasi Sıcaklığınin Renk Ve Toplam Klorofil Değişimi Üzerine Etkisi. Tarımsal Mekanizasyon 18. Ulusal Kongresi Tekirdağ.
- Spanos GA, Wrolstad RE 1992. Phenolic of Apple, Pear and White Grape Juices and Their Changes with Processing and Storage. Journal of Agricultural and Food Chemistry 40(9):1478-1487.
- Toor RK, Savage GP 2006. Effect of Semi-Drying on The Antioxidant Components of Tomatoes. Food Chemistry. 94(1):90-97.
- Viapiana A, Konopacka A, Waleron K, Wesolowski M 2017. Cistus incanus L. Commercial Products as a Good Source of Polyphenols in Human Diet. Industrial Crops and Products, 107: 297-304.
- Yilmaz A, Alibas I, Asik BB 2021. The Effect of Drying Methods on the Color, Chlorophyll, Total Phenolic, Flavonoids, and Macro and Micronutrients of Thyme Plant. Journal of Food Processing and Preservation. 45(11):15915.
Alanya Ekolojik Koşullarında Yetişen Cistus creticus L. Bitkisinin Taze ve Kuru Yaprak Örneklerinde Bazı Biyoaktif Bileşenlerin, Pigment İçeriği ve Antioksidan Kapasitenin Belirlenmesi
Öz
Cistus creticus L. Türkiye’nin kuzey, güney ve batı kıyıları boyunca yayılım gösteren önemli tıbbi bitkilerden birisidir. Antik çağlardan beri antimikrobiyal, antiinflamatuar, sitotoksik ve antiülserojenik özellikleri nedeniyle halk tıbbında kullanılmaktadır. Bitkinin taze ve kuru yaprakları genellikle çay olarak tüketilmektedir. Bu çalışma, Alanya ekolojik koşullarında doğal olarak yetişen Cistus creticus L. bitkisinin taze ve kurutulmuş yapraklarının bazı biyokimyasal özelliklerini araştırmayı amaçlamaktadır. Bu amaçla bitkinin vejetatif gelişiminin en yüksek olduğu ilkbahar döneminde hasat edilen yaprak örneklerinde; toplam askorbik asit, toplam fenolik, toplam flavonoid, toplam klorofil ve toplam karotenoid içerikleri ile antioksidan aktivite araştırılmıştır. Toplam askorbik asit miktarı dışındaki tüm parametreler kuru yaprak örneklerinde taze yaprak örneklerinden daha yüksek bulunmuştur. Bitkinin yüksek derecede biyoaktif bileşen içermesi ve % 50 civarında radikal süpürücü aktiviteye sahip olması bakımından tüketiminin önemli olduğu düşünülmektedir.
Anahtar Kelimeler
Antioksidan kapasite Fenolik Karotenoid Klorofil Cistus creticus
Kaynakça
- Acıbuca V, Bostan Budak D 2018. Dünya’da ve Türkiye’de Tıbbi ve Aromatik Bitkilerin Yeri ve Önemi. Çukurova Tarım Gıda Bil. Der. 33(1): 37-44.
- Akkol EK., Orhan IE, Yesilada E 2012. Anticholinesterase and antioxidant Effects of The Ethanol Extract, Ethanol Fractions And Isolated Flavonoids from Cistus laurifolius L. Leaves. Food Chemistry. 131(2): 626-631.
- Amensour M, Sendra E, Pérez-Alvarez JA, Skali-Senhaji N, Abrini J, Fernandez Lopez J 2010. Antioxidant Activity and Chemical Content of Methanol and Ethanol Extracts From Leaves of Rockrose (Cistus ladaniferus). Plant Foods Hum Nutr. 65:170–178.
- Anonim 2021. Konya Gıda ve Tarım Üniversitesinden Yerli Pastil. https://www.dha.com.tr/ saglikyasam/ konya-gida-ve-tarim-universitesinden-yerli pastil/haber-1808546 (Alınma Tarihi: 04.11.2021)
- AOAC, 1990. Official Methods of Analysis of The Association of Official Analytical Chemists 15th ed., Arlington VA, USA, 1058-1059.
- Bakhshi D, Arakawa O 2006. Effects of UV-B Irradiation on Phenolic Compound Accumulation And Antioxidant Activity in ‘Jonathan’ Apple Influenced by Bagging, Temperature and Maturation. Journal of Food, Agriculture & Environment. 4(1): 75-79.
- Barrajón-Catalán E, Tomás-Menor L, Morales-Soto A, Bruňá NM, López DS, Segura-Carretero A, Micoli V 2016. Rockroses (Cistus sp.) Oils. Essential Oils in Food Prevention. Flavor Saf, 74: 649-657.
- Benzie IFF, Strain JJ 1996. The Ferric Reducing Ability of Plasma (FRAB) as a Measure of Antioxidant Power: The FRAB Assay, Analytical Biochemistry, 239: 70-76.
- Davis PH 1965. Flora of Turkey and the East Aegean Islands, I. Edinburgh: Edinburgh Univ Press.
- De-Kok L, Graham M 1980. Levels of Pigments, Soluble Proteins, Amino Acids and Sulfhydryl Compounds in Foliar Tissue of Arabidopsis thaliana During Dark İnduced And Natural Senescence. Plant Physiology and Biochemistry. 27: 133-142.
- Dimcheva V, Karsheva M 2017. Antioxidant Activity and Polyphenolic Content of The Bulgarian Wild Herb Cistus incanus Stored Under Different Conditions. J Chem Technol Metall 52(5):781-790.
- El Euch SK., Bouajila J, Bouzouita N 2015.Chemical Composition, Biological and Cytotoxic Activities of Cistus salviifolius Flower Buds and Leaves Extracts. Industrial Crops and Products 76:1100–1105.
- Piluzza G, Bullitta S 2011. Correlations Between Phenolic Content And Antioxidant Properties in Twenty-Four Plant Species of Traditional Ethnoveterinary Use in the Mediterranean Area. Pharm. Biol. 49 (3): 240–247 .
- Gawel-Beben K, Kukula-Koch W, Hoian U, Czop M, Strzepek-Gomolka, M, Antosiewicz B 2020. Characterization of Cistus incanus L. and Cistus ladanifer L. Extracts As Potential Multifunctional Antioxidant Ingredients For Skin Protecting Cosmetics. Antioxidants. 9(3): 202.
- Guimarães R, Barros L, Carvalho AM, Sousa MJ, Morais JS, Ferreira IC 2009. Aromatic Plants as a Source of Important Phytochemicals: Vitamins, Sugars and Fatty Acids in Cistus ladanifer, Cupressus lusitanica and Eucalyptus gunnii Leaves. Industrial crops and products. 30(3): 427-430.
- Guvenc A, Yildiz S, Ozkan AM, Erdurak CS, Coşkun M, Yılmaz G, Okuyama T, Okada Y 2005. Antimicrobiological Studies on Turkish Cistus Species. Pharmaceutical Biology, 43(2): 178-183.
- Joshi APK., Rupasinghe HPV, Khanizadeh S 2011. Impact of Drying Processes on Bioactive Phenolics, Vitamin C and Antioxidant Capacity of Red‐Fleshed Apple Slices. Journal of Food Processing and Preservation. 35(4): 453-457.
- Kamiloglu S, Capanoglu E 2015. Polyphenol Content in Figs (Ficus carica L.): Effect of Sun-drying. International Journal of Food Properties, 18(3): 521-535.
- Kilic DD, Siriken B, Erturk O, Tanrikulu G, Melek Gul, Başkan C 2019. Antibacterial, Antioxidant and DNA Interaction Properties of Cistus creticus L. Extracts. Journal of International Environmental Application and Science, 14(3): 110-115.
- Kose MD, Tekin BN, Bayraktar O, Duman ET, Başpınar Y 2017. Antioxidant and Antimicrobial Properties of Cistus ladanifer. International Journal of Secondary Metabolite, 4(3, Special Issue 2): 434-444.
- Kumar SS, Manoj P, Shetty NP, Giridhar P 2015. Effect of Different Drying Methods on Chlorophyll, Ascorbic Acid and Antioxidant Compounds Retention of Leaves of Hibiscus sabdariffa L. Journal of the Science of Food and Agriculture. 95(9):1812-1820.
- Lahcen SA, El Hattabi L, Benkaddour R, Chahboun N, Ghanmi M, Satrani B, Zarrouk A 2020. Chemical Composition, Antioxidant, Antimicrobial and Antifungal Activity of Moroccan Cistus creticus Leaves. Chemical Data Collections. 26: 100346.
- Lanfer-Marquez UM, Barros RMC, Sinnecker P 2005. Antioxidant activity of chlorophylls and their derivatives. Food Research International 38(8-9): 885-891.
- Lichtenthaler K, Welburn AR 1983. Determination of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents. Botanisches Institutder Univeristat, Kaiserstran βe 12, Postfach 591-592.
- Liu X, Wang H, Chen Z 2016. Effect of Carotenoids on Body Colour of Discus Fish (Symphysodon aequifasciatus axelrodi Schultz, 1960). Aquaculture Research. 47(4): 130.
- Murathan ZT, Kaya A 2020. Alanya Ekolojik Koşullarında Yetiştirilen Hass ve Fuerte Avokado Çeşitlerinin Bazı Fitokimyasal İçerikleri ile Antioksidan Aktivitelerinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 23(6): 1435-1440.
- Nindo C, Sun T, Wang SW, Tang J , Powers JR 2003. Evaluation of Drying Technologies for Retention of Physical Quality and Antioxidants in Asparagus (Asparagus officinalis, L.). LWT-Food Science and Technology. 36(5): 507-516.
- Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet J, Luyck M, Cazin M, Cazin J.C, Bailleul F, Trotin F 2000. Phenolic Compounds and Antioxidant Activities of Buckwheat (Fagopyrum esculentum Moench) Hulls and Flour. Journal of Ethnopharmacology. 72: 35-40.
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26(9-10): 1231- 1237.
- Riehle P, Rohn S 2013. Phenolic Compounds in Cistus incanus Herbal Infusion-Antioxidant Capacity and Thermal Stability During The Brewing Process. Food Research International. 53(2): 891-899.
- Sekeroglu N, Gezici S 2021. Rockrose (Cistus spp.) Species as Turkey’s Virus Repellent Plants: Traditional Uses, Bioactive Chemical Components and Pharmacological Activities. Lokman Hekim Dergisi. 11(2):258-268.
- Soysal Y, Oztekin S 1998. Mentha piperita (Tibbi Nane)'de Kurutma Havasi Sıcaklığınin Renk Ve Toplam Klorofil Değişimi Üzerine Etkisi. Tarımsal Mekanizasyon 18. Ulusal Kongresi Tekirdağ.
- Spanos GA, Wrolstad RE 1992. Phenolic of Apple, Pear and White Grape Juices and Their Changes with Processing and Storage. Journal of Agricultural and Food Chemistry 40(9):1478-1487.
- Toor RK, Savage GP 2006. Effect of Semi-Drying on The Antioxidant Components of Tomatoes. Food Chemistry. 94(1):90-97.
- Viapiana A, Konopacka A, Waleron K, Wesolowski M 2017. Cistus incanus L. Commercial Products as a Good Source of Polyphenols in Human Diet. Industrial Crops and Products, 107: 297-304.
- Yilmaz A, Alibas I, Asik BB 2021. The Effect of Drying Methods on the Color, Chlorophyll, Total Phenolic, Flavonoids, and Macro and Micronutrients of Thyme Plant. Journal of Food Processing and Preservation. 45(11):15915.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Aralık 2022 |
| Gönderilme Tarihi | 30 Kasım 2021 |
| Kabul Tarihi | 24 Şubat 2022 |
| Yayımlandığı Sayı | Yıl 2022Cilt: 25 Sayı: Ek Sayı 1 |
Kaynak Göster
2022-JIF = 0.500
2022-JCI = 0.170
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