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Investigation of the Cytotoxic Effect of Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Species Collected in Different Years

Yıl 2023, Cilt: 26 Sayı: 6, 1268 - 1276, 31.12.2023

Orhan Ünal , Serap Özkaya , Esra Aydemir , Mehmet Torun

https://doi.org/10.18016/ksutarimdoga.vi.1205655

Öz

Cancer is a systemic disease that occurs with the accumulation of many mutations in the genome. Due to the different treatment methods used in cancer treatments, it prevents the success of the treatments due to the high toxicity and drug resistance in the cells over time. Therefore, natural products and their synthetic derivatives have great potential for cancer treatment. Benli Şalba (Salvia pisidica Boiss.&Heldr. ex Bentham) has many biological activities, especially antibacterial and antioxidative effects. In this study, the cytotoxic effects of water extracts of plants collected in different years against breast cancer cells were investigated. In this study, 24, 48 and 72 hour incubations were made in MCF-7, MDA-MB-231, and MCF10-A cell lines. The water extracts of S.pisidica plant collected in 2017, 2018 and 2019 were applied to the cells at doses of 1-1000 µg/mL. At the end of the incubation period, cytotoxicity test was performed with the WST-8 cell viability determination kit. In the results of the experiment, it was calculated that the IC50 value of the extract of 2017 was 1.95 ng/mL in 48 hours of incubation, and the IC40 value of the extract of 2019 was 3.9 and 1.95 µg/mL in 48 hours of incubation in MCF-7 cells. In the more aggressive MDA cells, the IC50 value of the 2019 S.pisidica water extract in 48 hours of incubation is 7.8 µg/mL. When the results obtained were examined, it was found that the phenolic contents differed depending on the year and were low in the samples of 2017 and high in the samples of 2019.

Anahtar Kelimeler

Salvia pisidica Lamiaceae Cancer Cytotoxicity Ecology

Kaynakça

  • Abu-Dahab, R., Abdallah, M.R., Kasabri, V., Mhaidat, N.M.,& Afifi, F.U. (2014). Mechanistic studies of antiproliferative effects of Salvia triloba and Salvia dominica (Lamiaceae) on breast cancer cell lines (MCF7 and T47D). Zeitschrift für Naturforschung C, 69(11-12), 443-451.
  • Atak, E., & Uslu, M.E. (2018). Fenolik Bileşikler, Ekstraksiyon Metotları ve Analiz Yöntemleri. MCBÜ Soma Meslek Yüksekokulu Teknik Bilimler Dergisi, 27(3), 39-48.
  • Barjaktarevic, A., Ćirović, N., Arsenijevic, N., Volarevic, V., Markovic, B., Mitić, V., Jovanic, V.S., & Cupara, S. (2021). Antioxidant, Antimicrobial and Cytotoxic Activities of Salvia verticillata L. Extracts. Indian Journal of Pharmaceutical Sciences. 83(6), 1280-1287.
  • Başyiğit, M., & Baydar, M. (2017). Tıbbi Adaçayı (Salvia officinalis L.)’nda Farklı Hasat Zamanlarının Uçucu Yağ ve Fenolik Bileşikler ile Antioksidan Aktivite Üzerine Etkisi. Süleyman Demirel University Journal of Natural and Applied Sciences. 21(1), 131‐137.
  • Behçet, L., & Yapar, Y. (2020). Türkiye’den Bazı Kareler İçin Yeni Floristik Kayıtlar. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 9(4):1482-1495.
  • Cai, J., Chen, S., Zhang, W., Zheng, X., Hu, S., Pang, C., Lu, J., Xing, J., & Dong, Y. (2014). Salvianolic acid A reverses paclitaxel resistance in human breast cancer MCF-7 cells via targeting the expression of transgelin 2 and attenuating PI3 K/Akt pathway. Phytomedicine. 21(12), 1725-1732.
  • Chen, X., Yu, J., Zhong, B., Lu, J., Lu, J.J., Li, S., & Lu, Y. (2019). Pharmacological activities of dihydrotanshinone I, a natural product from Salvia miltiorrhiza Bunge. Pharmacological Research. 145, 104254-104254.
  • Çelik, S., Konkan, R., Erkme, & H. (2007). Tabo A, Erkıran M. Bitkisel ilaçlar ve psikiyatride kullanımı. Düşünen Adam. 20(4), 186-195.
  • Deveci, H.A., Gökhan, N., Kırpık, M.A., Harmankaya, A., & Yıldız, Y. (2016). Fenolik Bileşik İçeren Bitkisel Antioksidanlar. Kafkas Üniversitesi Fen Bil. Enst. Derg. 9(1), 26-32.
  • Eghbaliferiz, S., Soheili, V., Tayarani-Najaran, Z., Asili, J. (2019). Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran. Physiology and Molecular Biology of Plants. 25(4), 1083-1089.
  • ElKhazendar, M., Chalak, J., El-Huneidi, W., Vinod, A., Abdel-Rahman, W.M., & Abu-Gharbieh, E. (2019). Antiproliferative and proapoptotic activities of ferulic acid in breast and liver cancer cell lines. Tropical Journal of Pharmaceutical Research. 18(12), 2571-2576.
  • Ferlay, J., Colombet, M., Soerjomataram, I., Parkin, D.M., Piñeros, M., Znaor, A., & Bray, F. (2021). Cancer statistics for the year 2020: An overview. International Journal of Cancer. 149(4), 778–789. https://doi.org/10.1002/IJC.33588
  • Fialová, S., Tekeľová, D., Rendeková, K., Klinčok, J., Kolárik, M., Kurucová, K., & Grančai, D. (2015). Phenolic compounds variation in Mentha L. species in the course of a four-years period Kolísanie fenolových látok v rôznych druhoch Mentha L. počas 4 ročného obdobia. Acta Fac. Pharm. Univ. Comen. LXII, (Suppl IX), 2-7. ISSN 1338-6786 (online) and ISSN 0301-2298 (print version), DOI: 10.1515/afpuc-2015-0013.
  • Foulkes, W.D., Smith, I.E., & Reis, J.S. (2010). "Triple-Negative Breast Cancer." New England Journal of Medicine. 363(20), 1938-1948.
  • Greenwell, M., & Rahman, P.K.S.M. (2015). Medicinal Plants: Their Use in Anticancer Treatment. Int J Pharm Sci Res. 6(10), 4103–4112. doi: 10.13040/IJPSR.0975-8232.6(10).4103-12.
  • Hedge, I.C. (1982). Salvia P.H. Davis (Ed.), Flora of Turkey and the East Aegean islands, Edinburgh University Press, Edinburgh. 7, 400-461
  • Lampronti, İ.,& Saab, A.M., Gambari, R. (2006). Magnoliophyta Bölümüne Ait Bitkilerden Elde Edilen Uçucu Yağların Antiproliferatif Aktivitesi. Int. J. Oncol. 29, 989-995.
  • Levenson, A.S., & Jordan, V.C. (1997). "MCF-7: the first hormoneresponsive breast cancer cell line." Cancer Res. 57, 3071–3078.
  • Mladkova, J., Sanda, M., Matouskova, E., & Selicharova, I. (2010). "Phenotyping breast cancer cell lines EM-G3, HCC1937, MCF7 and MDA-MB-231 using 2-D electrophoresis and affinity chromatography for glutathione-binding proteins." BMC Cancer 10, 449. https://doi.org/10.1186/1471-2407-10-449.
  • Özkan, G., Sağdıç, O., Göktürk, R.S., Ünal, O., Albayrak, S. (2010). Study on chemical composition and biological activities of essential oil and extract from Salvia pisidica. LWT-Food Scıence And Technology. 43(1), 186-190.
  • Quan, G., Chen, W., Wen, Y., Yan, Y., Gu, M., & Pan, Y. (2019). Chemoprotective efficacy of salvianolic acid B via triggering apoptosis in MCF-7 human breast cancer cells. International Journal of Pharmacology. 15(1), 110-115.
  • Rustaie, A., Hadjiakhoondi, A., Akbarzadeh, T., Safavi, M., Samadi, N., & Sabourian, R. (2018). Phytochemical constituents and biological activities of Salvia suffruticosa. Research Journal of Pharmacognosy. 5(2), 25-32.
  • Saleh, A., ElFayoumi, H.M., Youns, M., & Barakat, W. (2019). Rutin and orlistat produce antitumor effects via antioxidant and apoptotic actions. Naunyn-Schmiedeberg's Archives of Pharmacology. 392(2), 165-175.
  • Siegel, R.L., Miller, K.D., & Jemal, A. (2019). Cancer statistics, CA: A Cancer. Journal for Clinicians. 69(1), 7-34.
  • Taştan, P., Birim, D., Fafal, T., Armağan, G., & Kıvçak, B. (2022). Bazı Lamiaceae Bitkilerinin Yağ Asidi Bileşiminin Belirlenmesi. KSÜ Tarım ve Doğa Derg 25 (6), 1199-1204. https://doi.org/10.18016/ ksu tarimdoga.vi.984818.
  • Torun, M., Rácz, G., Fogarassy, E., Vatai, G., Dinçer, C., Topuz, A., & Özdemir, O. (2014). Concentration of sage (Salvia fruticosa Miller) extract by using integrated membrane process. Separation and Purification Technology. 132, 244-251.
  • Tsai, S.L., Suk, F.M., Wang, C.I., Liu, D.Z., Hou, W.C., Lin, P.J., Hung, L.F., & Liang, Y.C. (2007). Anti-tumor potential of 15, 16-dihydrotanshinone I against breast adenocarcinoma through inducing G1 arrest and apoptosis. Biochemical Pharmacology. 74(11), 1575-1586.
  • Wu, Y., Xu, S., & Tian, X.Y. (2020). The effect of salvianolic acid on vascular protection and possible mechanisms. Oxidative Medicine and Cellular Longevity. 5472096. doi: 10.1155/2020/5472096.
  • Yang, S., Zhang, H., Yang, X., Zhao, H., & Zhu, Y. (2017). Evaluation of antiproliferative activities of rutin on human colon cancer lovo cells and breast cancer MCF-7 cells. Analytical and Quantitative Cytopathology and Histopathology. 39(2),99-107.
  • Yeh, C.N., Wu, R.C., Cheng, C.T., Tsai, C.Y., Chang, Y.R., Yeh, T.S., Wu, T.H., Lee, W.C., Chiang, K.C. (2018). HO-1 Is a Favorable Prognostic Factor for HBV-HCC Patients Who Underwent Hepatectomy. Cancer Manag Res, 10, 6049–6059, doi:10.2147/CMAR.S186931.
  • Zhong, J., Reece, E.A., Yang, P. (2015). Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects. Biochemical and biophysical research communications. 467(2), 179-184.

Farklı Yıllarda Toplanan Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Türünün Sitotoksik Etkisinin Araştırılması

Yıl 2023, Cilt: 26 Sayı: 6, 1268 - 1276, 31.12.2023

Orhan Ünal , Serap Özkaya , Esra Aydemir , Mehmet Torun

https://doi.org/10.18016/ksutarimdoga.vi.1205655

Öz

Kanser, genomdaki birçok mutasyonun birikimi ile ortaya çıkan sistemik bir hastalıktır. Kanser tedavilerinde kullanılan farklı tedavi yöntemleri nedeniyle zamanla hücrelerde yüksek toksisite ve ilaç direncine neden olması sebebiyle tedavilerdeki başarıyı engellemektedir. Bu nedenle kanser tedavisi için doğal ürünler ve bunların sentetik türevleri büyük bir potansiyel taşımaktadır. Benli Şalba (Salvia pisidica Boiss.&Heldr. Ex Bentham) antibakteriyel ve antioksidatif etki başta olmak üzere birçok biyolojik aktiviteye sahiptir. Bu çalışmada farklı yıllarda toplanan bitkileren elde edilen su ekstraktlarının meme kanser hücrelerinde sergilediği sitotoksik etkiler incelenmiştir. Çalışmada 24, 48 ve 72 saatlik inkübasyonlar MCF-7, MDA-MB-231 ve MCF10-A hücre hatlarında yapılmıştır. 2017, 2018 ve 2019 yıllarında toplanan S.pisidica bitkisinin su ekstraktları 1-1000 ng/mL dozlarda hücrelere uygulanmıştır. WST-8 hücre canlılığı belirleme kiti ile sitotoksisite testi inkübasyon süreleri sonunda yapılmıştır. Çalışmalar sonucunda MCF-7 hücrelerinde 2017 yılına ait ekstraktın 48 saatlik inkübasyonda IC50 değeri 1.95 ng/mL, 2019 yılına ait ekstraktın 48 saatlik inkübasyonda IC40 değeri 3.9 ve 1.95 ng/mL olduğu hesaplanmıştır. Daha agresif olan MDA hücrelerinde 2019 yılına ait S.pisidica su ekstraktının 48 saatlik inkübasyondaki IC50 değeri ise 7.8 ng/mL’dir. Elde edilen sonuçlara bakıldığında fenolik içeriklerin yıllara bağlı olarak farklılık gösterdiği ve 2017 örneğinde düşük 2019 örneğinde ise yüksek değerlerde olduğu bulunmuştur.

Anahtar Kelimeler

Lamiaceae Salvia pisidica Kanser Sitotoksisite Ekoloji

Kaynakça

  • Abu-Dahab, R., Abdallah, M.R., Kasabri, V., Mhaidat, N.M.,& Afifi, F.U. (2014). Mechanistic studies of antiproliferative effects of Salvia triloba and Salvia dominica (Lamiaceae) on breast cancer cell lines (MCF7 and T47D). Zeitschrift für Naturforschung C, 69(11-12), 443-451.
  • Atak, E., & Uslu, M.E. (2018). Fenolik Bileşikler, Ekstraksiyon Metotları ve Analiz Yöntemleri. MCBÜ Soma Meslek Yüksekokulu Teknik Bilimler Dergisi, 27(3), 39-48.
  • Barjaktarevic, A., Ćirović, N., Arsenijevic, N., Volarevic, V., Markovic, B., Mitić, V., Jovanic, V.S., & Cupara, S. (2021). Antioxidant, Antimicrobial and Cytotoxic Activities of Salvia verticillata L. Extracts. Indian Journal of Pharmaceutical Sciences. 83(6), 1280-1287.
  • Başyiğit, M., & Baydar, M. (2017). Tıbbi Adaçayı (Salvia officinalis L.)’nda Farklı Hasat Zamanlarının Uçucu Yağ ve Fenolik Bileşikler ile Antioksidan Aktivite Üzerine Etkisi. Süleyman Demirel University Journal of Natural and Applied Sciences. 21(1), 131‐137.
  • Behçet, L., & Yapar, Y. (2020). Türkiye’den Bazı Kareler İçin Yeni Floristik Kayıtlar. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 9(4):1482-1495.
  • Cai, J., Chen, S., Zhang, W., Zheng, X., Hu, S., Pang, C., Lu, J., Xing, J., & Dong, Y. (2014). Salvianolic acid A reverses paclitaxel resistance in human breast cancer MCF-7 cells via targeting the expression of transgelin 2 and attenuating PI3 K/Akt pathway. Phytomedicine. 21(12), 1725-1732.
  • Chen, X., Yu, J., Zhong, B., Lu, J., Lu, J.J., Li, S., & Lu, Y. (2019). Pharmacological activities of dihydrotanshinone I, a natural product from Salvia miltiorrhiza Bunge. Pharmacological Research. 145, 104254-104254.
  • Çelik, S., Konkan, R., Erkme, & H. (2007). Tabo A, Erkıran M. Bitkisel ilaçlar ve psikiyatride kullanımı. Düşünen Adam. 20(4), 186-195.
  • Deveci, H.A., Gökhan, N., Kırpık, M.A., Harmankaya, A., & Yıldız, Y. (2016). Fenolik Bileşik İçeren Bitkisel Antioksidanlar. Kafkas Üniversitesi Fen Bil. Enst. Derg. 9(1), 26-32.
  • Eghbaliferiz, S., Soheili, V., Tayarani-Najaran, Z., Asili, J. (2019). Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran. Physiology and Molecular Biology of Plants. 25(4), 1083-1089.
  • ElKhazendar, M., Chalak, J., El-Huneidi, W., Vinod, A., Abdel-Rahman, W.M., & Abu-Gharbieh, E. (2019). Antiproliferative and proapoptotic activities of ferulic acid in breast and liver cancer cell lines. Tropical Journal of Pharmaceutical Research. 18(12), 2571-2576.
  • Ferlay, J., Colombet, M., Soerjomataram, I., Parkin, D.M., Piñeros, M., Znaor, A., & Bray, F. (2021). Cancer statistics for the year 2020: An overview. International Journal of Cancer. 149(4), 778–789. https://doi.org/10.1002/IJC.33588
  • Fialová, S., Tekeľová, D., Rendeková, K., Klinčok, J., Kolárik, M., Kurucová, K., & Grančai, D. (2015). Phenolic compounds variation in Mentha L. species in the course of a four-years period Kolísanie fenolových látok v rôznych druhoch Mentha L. počas 4 ročného obdobia. Acta Fac. Pharm. Univ. Comen. LXII, (Suppl IX), 2-7. ISSN 1338-6786 (online) and ISSN 0301-2298 (print version), DOI: 10.1515/afpuc-2015-0013.
  • Foulkes, W.D., Smith, I.E., & Reis, J.S. (2010). "Triple-Negative Breast Cancer." New England Journal of Medicine. 363(20), 1938-1948.
  • Greenwell, M., & Rahman, P.K.S.M. (2015). Medicinal Plants: Their Use in Anticancer Treatment. Int J Pharm Sci Res. 6(10), 4103–4112. doi: 10.13040/IJPSR.0975-8232.6(10).4103-12.
  • Hedge, I.C. (1982). Salvia P.H. Davis (Ed.), Flora of Turkey and the East Aegean islands, Edinburgh University Press, Edinburgh. 7, 400-461
  • Lampronti, İ.,& Saab, A.M., Gambari, R. (2006). Magnoliophyta Bölümüne Ait Bitkilerden Elde Edilen Uçucu Yağların Antiproliferatif Aktivitesi. Int. J. Oncol. 29, 989-995.
  • Levenson, A.S., & Jordan, V.C. (1997). "MCF-7: the first hormoneresponsive breast cancer cell line." Cancer Res. 57, 3071–3078.
  • Mladkova, J., Sanda, M., Matouskova, E., & Selicharova, I. (2010). "Phenotyping breast cancer cell lines EM-G3, HCC1937, MCF7 and MDA-MB-231 using 2-D electrophoresis and affinity chromatography for glutathione-binding proteins." BMC Cancer 10, 449. https://doi.org/10.1186/1471-2407-10-449.
  • Özkan, G., Sağdıç, O., Göktürk, R.S., Ünal, O., Albayrak, S. (2010). Study on chemical composition and biological activities of essential oil and extract from Salvia pisidica. LWT-Food Scıence And Technology. 43(1), 186-190.
  • Quan, G., Chen, W., Wen, Y., Yan, Y., Gu, M., & Pan, Y. (2019). Chemoprotective efficacy of salvianolic acid B via triggering apoptosis in MCF-7 human breast cancer cells. International Journal of Pharmacology. 15(1), 110-115.
  • Rustaie, A., Hadjiakhoondi, A., Akbarzadeh, T., Safavi, M., Samadi, N., & Sabourian, R. (2018). Phytochemical constituents and biological activities of Salvia suffruticosa. Research Journal of Pharmacognosy. 5(2), 25-32.
  • Saleh, A., ElFayoumi, H.M., Youns, M., & Barakat, W. (2019). Rutin and orlistat produce antitumor effects via antioxidant and apoptotic actions. Naunyn-Schmiedeberg's Archives of Pharmacology. 392(2), 165-175.
  • Siegel, R.L., Miller, K.D., & Jemal, A. (2019). Cancer statistics, CA: A Cancer. Journal for Clinicians. 69(1), 7-34.
  • Taştan, P., Birim, D., Fafal, T., Armağan, G., & Kıvçak, B. (2022). Bazı Lamiaceae Bitkilerinin Yağ Asidi Bileşiminin Belirlenmesi. KSÜ Tarım ve Doğa Derg 25 (6), 1199-1204. https://doi.org/10.18016/ ksu tarimdoga.vi.984818.
  • Torun, M., Rácz, G., Fogarassy, E., Vatai, G., Dinçer, C., Topuz, A., & Özdemir, O. (2014). Concentration of sage (Salvia fruticosa Miller) extract by using integrated membrane process. Separation and Purification Technology. 132, 244-251.
  • Tsai, S.L., Suk, F.M., Wang, C.I., Liu, D.Z., Hou, W.C., Lin, P.J., Hung, L.F., & Liang, Y.C. (2007). Anti-tumor potential of 15, 16-dihydrotanshinone I against breast adenocarcinoma through inducing G1 arrest and apoptosis. Biochemical Pharmacology. 74(11), 1575-1586.
  • Wu, Y., Xu, S., & Tian, X.Y. (2020). The effect of salvianolic acid on vascular protection and possible mechanisms. Oxidative Medicine and Cellular Longevity. 5472096. doi: 10.1155/2020/5472096.
  • Yang, S., Zhang, H., Yang, X., Zhao, H., & Zhu, Y. (2017). Evaluation of antiproliferative activities of rutin on human colon cancer lovo cells and breast cancer MCF-7 cells. Analytical and Quantitative Cytopathology and Histopathology. 39(2),99-107.
  • Yeh, C.N., Wu, R.C., Cheng, C.T., Tsai, C.Y., Chang, Y.R., Yeh, T.S., Wu, T.H., Lee, W.C., Chiang, K.C. (2018). HO-1 Is a Favorable Prognostic Factor for HBV-HCC Patients Who Underwent Hepatectomy. Cancer Manag Res, 10, 6049–6059, doi:10.2147/CMAR.S186931.
  • Zhong, J., Reece, E.A., Yang, P. (2015). Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects. Biochemical and biophysical research communications. 467(2), 179-184.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Yapısal Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Orhan Ünal 0000-0001-9633-5726

Serap Özkaya 0000-0002-7071-4805

Esra Aydemir 0000-0002-5206-7333

Mehmet Torun 0000-0002-6287-2993

Erken Görünüm Tarihi 14 Haziran 2023
Yayımlanma Tarihi 31 Aralık 2023
Gönderilme Tarihi 16 Kasım 2022
Kabul Tarihi 5 Mayıs 2023
Yayımlandığı Sayı Yıl 2023Cilt: 26 Sayı: 6

Kaynak Göster

APA Ünal, O., Özkaya, S., Aydemir, E., Torun, M. (2023). Farklı Yıllarda Toplanan Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Türünün Sitotoksik Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1268-1276. https://doi.org/10.18016/ksutarimdoga.vi.1205655
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