Çukurova Bölgesi Turunçgil Üretim Alanlarında Citrus Yellow Vein Clearing Virus (CYVCV; Potexvirus citriflavivenae)’ünün Araştırılması ve İzolatlar Arası Benzerlik Oranlarının Belirlenmesi
Öz
Citrus yellow vein clearing virus (CYVCV; Potexvirus citriflavivenae) dünyada ilk kez Pakistan’da ardından Hindistan’da tespit edilmiştir. Türkiye’de ise 2000’li yıllarda bu hastalığın varlığı yeni bir turunçgil viral etmeni olarak bildirilmiştir. Bu dönemde virüsün ilk belirlendiği il olan Adana ilinde eradikasyon işlemi gerçekleştirilerek enfekteli ağaçlar kesilmiştir. CYVCV’nin tek sarmal pozitif RNA yapısında olduğu 2012 yılında ortaya konmuştur. CYVCV'nin Çukurova Bölgesi’ndeki durumunu araştırmak, etmenin enfeksiyon durumunu belirlemek ve izolatlar arasındaki farklılığı moleküler olarak saptamak amacıyla bu çalışma yürütülmüştür. Sörvey çalışmaları 2016-2022 yılları arasında Hatay, Adana ve Mersin illerini kapsayacak şekilde güdümlü sörvey çalışması olarak gerçekleştirilmiştir. Çalışma başta limon olmak üzere portakal, greyfurt ve turunç çeşitlerini kapsamıştır. Toplamda 150 turunçgil bahçesi ve 10 farklı fidan üretim alanında CYVCV’nin varlığına bakılmış ve 100 örnek RT-PCR yöntemiyle testlenmiştir. Örneklerin toplanması etmenin karakteristik yaprak simptomları göz önüne alınarak yapılmıştır. Alınan örneklerden TNA ekstraksiyonu CTAB tampon kullanılarak gerçekleştirilmiştir. RT-PCR çalışmaları sonucunda elde edilen amplifikonlar sekans analizine gönderilmiş, elde edilen baz dizilimleri NCBI veri tabanında BLAST metoduyla seçilen kayıtlı CYVCV izolatları ile karşılaştırılmıştır. Toplamda 35 örneğin CYVCV ile enfekteli olduğu belirlenmiştir. Enfekteli olarak belirlenen bu örneklere eradikasyon işlemi uygulanmıştır. Çalışma sonucunda izolatların benzerlik oranlarının yüksek olduğu (%97-99.8) tespit edilmiştir.
Kaynakça
- Alshami, A. A. A., Ahlawat, Y. S., & Pant, R. P. (2003). A hitherto unreported yellow vein clearing disease of citrus in India and its viral etiology. Indian Phytopathology, 56(4), 422-427.
- Anonim, (2017). Tarım ve Orman Bakanlığı Turunçgil sarı damar açılması virüsü survey talimatı https://www.tarimorman.gov.tr/GKGM/Belgeler/DB_Bitki_Sagligi/Survey/10Turuncgil_sari_damar_acilmasi virusu_Survey_Talimati_2017.pdf. (Alınma Tarihi: 06.05.2024).
- Bovey, R. (1965). Identification of viruses in clonally propagated plants having one or more viruses. Proceedings Conference on Virus And Vector on Perennial Hosts With Special Reference to Vitis, Davis, California, 1965, pp. 223-227.
- Bozan, O., & Önelge, N. (2018). Adana, Mersin ve Hatay illerinde Citrus chlorotic dwarf associated virus hastalığının yaygınlığı. Çukurova Tarım ve Gıda Bilimleri Dergisi, 33(1), 57-62.
- Bozdoğan, A.., Önelge N., (2016). Turunçgil sarı damar açılması (TSDAV) hastalığının Farklı turunçgil türlerinde moleküler olarak tanılanması (Tez no 621561). [Yüksek Lisans Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
- Catara, A., Azzaro, A., Davino, M., & Polizzi, G. (1993). Yellow vein clearing of lemon in Pakistan. Proceedings 12th Conference of the International Organzation of Citrus Virologist, New Delhi, India, 1993, pp. 364–367.
- Chen, H. M., Li, Z. A., Wang, X. F., Zhou, Y., Tang, K. Z., Zhou, C. Y., Zhao, X. Y., and Yue, J. Q. (2014). First report of Citrus yellow vein clearing virüs on Lemon in Yunnan, China. Plant Disease, 98(12), 1747-1747. https://doi.org/10.1094/PDIS-04-14-0343-PDN.
- EPPO, (2022). European and Mediterranean Plant Protection Organization. https://www.eppo.int/ACTIVITIES/plant_quarantine/alert_list_viruses/citrus_yellow_vein_clearing_virus. (Alınma Tarihi: 20.06.2024).
- Hashmian, B. S. M., & Aghajanzadeh, S. (2017). Occurrence of Citrus yellow vein clearing virus in citrus species in Iran. Journal of Plant Pathology, 99(1), 290.
- Hewitt W.B. and Gifford E.M. (1956). Symptoms for identifying Fanleaf in dormant grapevines. The Bulletin Deparment of Agriculture State of California, 45 (3), 249-252.
- Korkmaz, S., Çinar, A., Bozan, O., Kersting, U. (1994) Distribution and natural transmission of a new whitefly-borne virus disease of citrus in the Eastern Mediterranean Region of Turkey. In Proceedings of the 9th Congress of the Mediterranean Phytopathological Union, Aydın, 1994, pp 437–439.
- Loconsole, G., Önelge, N., Potere, O., Giampetruzzi, A., Bozan, O., Satar, S., De Stradis, A., Savino, V., Yokomi, R. K., and Saponari, M. (2012). Identification and characterization of Citrus yellow vein clearing virus, a putative new member of the genus Mandarivirus. Phytopathology, 102(12), 1168-1175. https://doi.org/10.1094/PHYTO-06-12-0140-R.
- Murray, M. G., & Thompson, W. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic acids research, 8(19), 4321-4326. https://doi.org/10.1093/nar/8.19.4321.
- Önelge, N. (2002). First report of yellow vein clearing of lemons in Turkey. Journal of Turkish Phytopathology 32, 53-55.
- Önelge, N., Satar, S., Elibuyuk, O., Bozan, O., & Kamberoolu, M. (2011). Transmission studies on Citrus yellow vein clearing virus. In 18th Conference of the International Organization of Citrus Virus, Brazil, 2011, pp. 11-14.
- Önelge, N., Bozan, O. and Gök Güler, P. (2016). First report of Citrus yellow vein clearing virus infecting new natural host plants in Turkey. Journal of Plant Pathology, 98(2), 369-377.
- Zhang, Y. H., Liu, C. H., Wang, Q., Wang, Y. L., Zhou, C. Y., & Zhou, Y. (2019). Identification of Dialeurodes citri as a vector of Citrus yellow vein clearing virus in China. Plant Disease, 103(1), 65-68. https://doi.org/10.1094/PDIS-05-18-0911-RE.
- Zhang, Y., Wang, Y., Wang, Q., Cao, M., Zhou, C., & Zhou, Y. (2018). Identification of Aphis spiraecola as a vector of Citrus yellow vein clearing virus. European Journal of Plant Pathology, 152, 841-844.
- Zhou, Y., Chen, H. M., Cao, M. J., Wang, X. F., Jin, X., Liu, K. H., & Zhou, C. Y. (2017). Occurrence, distribution, and molecular characterization of Citrus yellow vein clearing virus in China. Plant Disease, 101(1), 137-143. https://doi.org/10.1094/PDIS-05-16-0679-RE.
- Zhou, Y., Ma, D.D., Chen, H.M., Wang, X.F., He, S.G., and Zhou, C.Y. (2016). A rapid and efficient purification of Citrus yellow vein clearing virus by sucrose cushion ultracentrifugation. Journal of Plant Pathology 98(1), 159-161. http://www.jstor.org/stable/24892638.
Investigating the Citrus Yellow Vein Clearing Virus (CYVCV; Potexvirus citriflavivenae) in the Çukurova Region's Citrus Production Areas and Analyzing the Identity Rates Between Isolates
Öz
Prevalence of Citrus Yellow Vein Clearing Virus (CYVCV) Disease and Similarity Rates Among Isolates in Citrus Production Areas of the Eastern Mediterranean Region
ABSTRACT: Citrus yellow vein clearing disease was found for the first time in the world in Pakistan, followed by India. This disease was first identified as a new citrus viral disease in Turkey in the 2000s. During this time, eradication efforts were carried out in Adana, the province where the disease was originally discovered, with infected trees cut down. In 2012, it was determined that the causative agent of this disease was Citrus yellow vein-clearing virus (CYVCV) and it was defined as having a single-stranded positive RNA structure. This study aimed to determine CYVCV prevalence in the Çukurova Region, identify new infected locations, and analyze molecular variations between isolates. The survey studies were conducted at between 2016 and 2024, including the provinces of Hatay, Adana, and Mersin. The study focused on lemon and included grapefruit, orange, and sour orange species and variants. CYVCV was investigated using the RT-PCR method in 150 citrus parcels and 10 different seedling production locations, as well as 100 different samples. Samples were collected based on the symptoms of yellow vein clearing and leaf deformation caused by the agent on the leaves. CTAB buffer was used to extract total nucleic acids from the samples. The sequence analysis of the amplicons obtained from RT-PCR analyses was conducted and the resulting base sequences were compared with the registered CYVCVs chosen using the BLAST method in the NCBI database. A total of 35 samples were revealed infected with CYVCV. As a result of the study, it was determined that there was a high similarity between the isolates (97.1-99.8%).
Key Words: CYVCV, Turkiye, Citrus, RT-PCRCitrus yellow vein clearing virus (CYVCV; Potexvirus citriflavivenae) was first detected in Pakistan and then in India. In Türkiye, this disease was reported as a new citrus viral disease in the 2000s. In Adana province, where the virus was first detected, eradication was carried out and infected trees were cut down. In 2012, the single-stranded positive RNA structure of CYVCV was identified. The purpose of this investigation was to assess the current state of CYVCV in the Çukurova Region, ascertain the agent's current infection status, and identify molecular differences between the isolates. The survey studies were conducted between 2016 and 2022, including the provinces of Hatay, Adana, and Mersin. The study focused on lemon and included grapefruit, orange, and sour orange species. CYVCV was investigated using the RT-PCR method in 150 citrus parcels and 10 different seedling production locations, as well as 100 different samples. Samples were collected by taking into account the characteristic leaf symptoms of the agent. CTAB buffer was used to extract total nucleic acids from the samples. The sequence analysis of the amplicons obtained from RT-PCR analyses was conducted and the resulting base sequences were compared with the registered CYVCVs chosen using the BLAST method in the NCBI database. A total of 35 samples were revealed infected with CYVCV. These samples that were found to be contaminated underwent an eradication procedure. As a result of the study, it was determined that there was a high similarity between the isolates (97-99.8%).
Kaynakça
- Alshami, A. A. A., Ahlawat, Y. S., & Pant, R. P. (2003). A hitherto unreported yellow vein clearing disease of citrus in India and its viral etiology. Indian Phytopathology, 56(4), 422-427.
- Anonim, (2017). Tarım ve Orman Bakanlığı Turunçgil sarı damar açılması virüsü survey talimatı https://www.tarimorman.gov.tr/GKGM/Belgeler/DB_Bitki_Sagligi/Survey/10Turuncgil_sari_damar_acilmasi virusu_Survey_Talimati_2017.pdf. (Alınma Tarihi: 06.05.2024).
- Bovey, R. (1965). Identification of viruses in clonally propagated plants having one or more viruses. Proceedings Conference on Virus And Vector on Perennial Hosts With Special Reference to Vitis, Davis, California, 1965, pp. 223-227.
- Bozan, O., & Önelge, N. (2018). Adana, Mersin ve Hatay illerinde Citrus chlorotic dwarf associated virus hastalığının yaygınlığı. Çukurova Tarım ve Gıda Bilimleri Dergisi, 33(1), 57-62.
- Bozdoğan, A.., Önelge N., (2016). Turunçgil sarı damar açılması (TSDAV) hastalığının Farklı turunçgil türlerinde moleküler olarak tanılanması (Tez no 621561). [Yüksek Lisans Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
- Catara, A., Azzaro, A., Davino, M., & Polizzi, G. (1993). Yellow vein clearing of lemon in Pakistan. Proceedings 12th Conference of the International Organzation of Citrus Virologist, New Delhi, India, 1993, pp. 364–367.
- Chen, H. M., Li, Z. A., Wang, X. F., Zhou, Y., Tang, K. Z., Zhou, C. Y., Zhao, X. Y., and Yue, J. Q. (2014). First report of Citrus yellow vein clearing virüs on Lemon in Yunnan, China. Plant Disease, 98(12), 1747-1747. https://doi.org/10.1094/PDIS-04-14-0343-PDN.
- EPPO, (2022). European and Mediterranean Plant Protection Organization. https://www.eppo.int/ACTIVITIES/plant_quarantine/alert_list_viruses/citrus_yellow_vein_clearing_virus. (Alınma Tarihi: 20.06.2024).
- Hashmian, B. S. M., & Aghajanzadeh, S. (2017). Occurrence of Citrus yellow vein clearing virus in citrus species in Iran. Journal of Plant Pathology, 99(1), 290.
- Hewitt W.B. and Gifford E.M. (1956). Symptoms for identifying Fanleaf in dormant grapevines. The Bulletin Deparment of Agriculture State of California, 45 (3), 249-252.
- Korkmaz, S., Çinar, A., Bozan, O., Kersting, U. (1994) Distribution and natural transmission of a new whitefly-borne virus disease of citrus in the Eastern Mediterranean Region of Turkey. In Proceedings of the 9th Congress of the Mediterranean Phytopathological Union, Aydın, 1994, pp 437–439.
- Loconsole, G., Önelge, N., Potere, O., Giampetruzzi, A., Bozan, O., Satar, S., De Stradis, A., Savino, V., Yokomi, R. K., and Saponari, M. (2012). Identification and characterization of Citrus yellow vein clearing virus, a putative new member of the genus Mandarivirus. Phytopathology, 102(12), 1168-1175. https://doi.org/10.1094/PHYTO-06-12-0140-R.
- Murray, M. G., & Thompson, W. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic acids research, 8(19), 4321-4326. https://doi.org/10.1093/nar/8.19.4321.
- Önelge, N. (2002). First report of yellow vein clearing of lemons in Turkey. Journal of Turkish Phytopathology 32, 53-55.
- Önelge, N., Satar, S., Elibuyuk, O., Bozan, O., & Kamberoolu, M. (2011). Transmission studies on Citrus yellow vein clearing virus. In 18th Conference of the International Organization of Citrus Virus, Brazil, 2011, pp. 11-14.
- Önelge, N., Bozan, O. and Gök Güler, P. (2016). First report of Citrus yellow vein clearing virus infecting new natural host plants in Turkey. Journal of Plant Pathology, 98(2), 369-377.
- Zhang, Y. H., Liu, C. H., Wang, Q., Wang, Y. L., Zhou, C. Y., & Zhou, Y. (2019). Identification of Dialeurodes citri as a vector of Citrus yellow vein clearing virus in China. Plant Disease, 103(1), 65-68. https://doi.org/10.1094/PDIS-05-18-0911-RE.
- Zhang, Y., Wang, Y., Wang, Q., Cao, M., Zhou, C., & Zhou, Y. (2018). Identification of Aphis spiraecola as a vector of Citrus yellow vein clearing virus. European Journal of Plant Pathology, 152, 841-844.
- Zhou, Y., Chen, H. M., Cao, M. J., Wang, X. F., Jin, X., Liu, K. H., & Zhou, C. Y. (2017). Occurrence, distribution, and molecular characterization of Citrus yellow vein clearing virus in China. Plant Disease, 101(1), 137-143. https://doi.org/10.1094/PDIS-05-16-0679-RE.
- Zhou, Y., Ma, D.D., Chen, H.M., Wang, X.F., He, S.G., and Zhou, C.Y. (2016). A rapid and efficient purification of Citrus yellow vein clearing virus by sucrose cushion ultracentrifugation. Journal of Plant Pathology 98(1), 159-161. http://www.jstor.org/stable/24892638.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Fitopatoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Erken Görünüm Tarihi | 19 Aralık 2024 |
| Yayımlanma Tarihi | |
| Gönderilme Tarihi | 12 Ağustos 2024 |
| Kabul Tarihi | 19 Kasım 2024 |
| Yayımlandığı Sayı | Yıl 2024Cilt: 27 Sayı: Ek Sayı 2 (Suppl 2) |
Kaynak Göster
2022-JIF = 0.500
2022-JCI = 0.170
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