Comparison of Conventional PCR Method With Cefoxitin Disc Diffusion, Automated System and Isolation on Chromogenic MRSA Medium Methods for The Detection of Meticillin Resistance In Staphylococcus aureus Strains Isolated From Various Clinical Specimens
Abstract
Meticillin-resistant Staphylococcus
aureus (MRSA) is one of the most important infectious agents. Therefore,
fast and accurate diagnosis of MRSA is utmost important. Although mecA
gene detection by PCR is reference method, conventional methods are preferred
in routine practices due to simplicity. As conventional methods last 48-96
hours, several chromogenic media have been developed. Our objective was to
compare the methods used for meticillin resistance detection with PCR.
Forty-eight S. aureus strains isolated from various clinical specimens
were included. Of the 48 S. aureus strains, 19 were mecA gene-positive
and 29 were negative. mecA gene-positive 19 strains were also
meticillin-resistant by automated system and disk diffusion. On chromogenic
agar, 15 of 19 MRSA strains were meticillin-resistant and 4 were
meticillin-sensitive. Twentynine mecA gene-negative strains were
susceptible to meticillin by automatized system and disk diffusion. Among 29 mecA
gene-negative MSSA strains inoculated on chromogenic agar, 17 were
methicillin-resistant. According to our study, chromogenic media would be
ineffective to detected to meticillin-resistance because of low sensitivity and
specificity in routine.
Keywords
References
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Details
Primary Language
English
Subjects
-
Journal Section
Research Article
Authors
Esra Kaya
*
0000-0002-0732-6471
Türkiye
Kezban Tülay Yalçınkaya
0000-0002-6324-4585
Hacer Uğurlu
0000-0001-6126-5502
Publication Date
June 30, 2019
Submission Date
September 5, 2018
Acceptance Date
January 30, 2019
Published in Issue
Year 2019 Volume: 22 Number: 3
