Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Selim Demir; İbrahim Turan; Sema Mısır; Yüksel Aliyazıcıoğlu

doi:10.18016/ksutarimdoga.vi.497868

EN TR

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Abstract

Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3±1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC₅₀ values of the extract in the cancer cells range from 100.4 to 298.5 μg/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.

Keywords

Dorycnium pentaphyllum Ekstraktının İnsan Meme, Karaciğer ve Akciğer Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Öz

Fabaceae ailesine mensup bitkilerin sitotoksik etkilerini konu alan bazı çalışmalar bulunmakla birlikte, Dorycnium pentaphyllum'un sitotoksik etkisi ile ilgili sınırlı araştırma bulunmaktadır. Bu çalışmanın amacı, D. pentaphyllum ekstraktının antioksidan özelliklerinin ve çeşitli insan kanser hücreleri üzerindeki sitotoksik etkisini belirlenmesidir. Ekstraktın toplam fenolik içeriği kolorimetrik yöntem kullanılarak belirlendi. Ekstraktın insan akciğer (A549), karaciğer (HepG2) ve meme (MCF-7) kanser hücreleri ile normal insan fibroblast hücreleri üzerindeki sitotoksik etkisi MTT analizi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içerik değeri g örnek başına 140.3±1.1 mg gallik asit eşdeğeri olarak bulundu. Ekstraktın çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdiği belirlendi ve IC₅₀ değerleri 100.4-298.5 μg/mL arasında hesaplandı. Bu çalışma D. pentaphyllum ekstraktının kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. D. pentaphyllum'un fitokimyasal uygulamaları kanser tedavisinde umut verici yaklaşımları temsil edebilir.

Anahtar Kelimeler

References

Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Ayazoglu Demir E, Ozgen U, Alpay Karaoglu S, Aliyazicioglu Y, 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod, 11(3): 270-284.
Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, Bedir A, 2011. Preventive and protective effects of Turkish propolis on H2O2-induced DNA damage in foreskin fibroblast cell lines. Acta Biol Hung, 62(4): 388-396.
Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV, 2016. An overview on the role of dietary phenolics for the treatment of cancers. Nutr J, 15(1): 99.
Bencherchar I, Demirtas I, Altun M, Gul F, Sarri D, Benayache F, Benayache S, Mekkiou R, 2017. HPLC analysis, anti-oxidant activity of Genista ferox and its antiproliferative effect in HeLa cell line. Bangladesh J Pharmacol, 12: 260-267.
Bhanot A, Sharma R, Noolvi MN, 2011. Natural sources as potential anti-cancer agents: a review. Int J Phytomed, 3: 9-26.
Bremner P, Rivera D, Calzado MA, Obón C, Inocencio C, Beckwith C, Fiebich BL, Muñoz E, Heinrich M, 2009. Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators. J Ethnopharmacol, 124(2): 295-305.
Chaddha V, Jain PK, Dixit ND, Rathor H, Zama R, 2018. An importance of herbal medicines in treatment of cancer disease. Int J Pharm Sci Rev Res, 51(2): 80-82.
Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O, 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer, 68(1): 165-172.

Demir S, Turan I, Aliyazicioglu Y, 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. J BUON, 21(4): 883-888.
Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y, 2017a. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J, 21(1): 121-126.
Demir S, Turan I, Aliyazicioglu Y, Kilinc K, Ozer Yaman S, Ayazoglu Demir E, Arslan A, Mentese A, Deger O, 2017b. Morus rubra extract induces cell cycle arrest and apoptosis in human colon cancer cells through endoplasmic reticulum stress and telomerase. Nutr Cancer, 69(1): 74-83.
Demir S, Turan I, Aliyazicioglu R, OzerYaman S, Aliyazicioglu Y, 2018a. Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells. J Pharm Anal, 8(5): 307-311.
Demir S, Turan I, Aliyazicioglu Y, 2018b. Cytotoxic effect of Rhododendron luteum leaf extract on human cancer cell lines. KSU J Agric Nat, 21(6): 950-956.
Demir S, Turan I, Aliyazicioglu Y, 2019. Antioxidant properties of Primula vulgaris flower extract and its cytotoxic effect on human cancer cell lines. KSU J Agric Nat, 22(1): 78-84.
Gbaweng AJY, Daïrou H, Zingué S, Emmanuel T, Tchinda AT, Frédérich M, Mbafor JT, 2018. Excelsanone, a new isoflavonoid from Erythrina excelsa (Fabaceae), with in vitro antioxidant and in vitro cytotoxic effects on prostate cancer cells lines. Nat Prod Res, 1-9/ doi: 10.1080/14786419.2018. 1495639, In Press.
Hussein SAM, El-Mesallamy AMD, Souleman AMA, Mousa MA, 2016. Cytotoxic activity of bioactive compound from Caesalpinia ferrea Martius, Fabaceae. IJPPR, 8(12): 2080-2084.
Jantova S, Nagy M, Ruzeková L, Grancai D, 2001. Cytotoxic effects of plant extracts from the families Fabaceae, Oleaceae, Philadelphaceae, Rosaceae and Staphyleaceae. Phytother Res, 15(1): 22-25.
Kim C, Kim B, 2018. Anti-cancer natural products and their bioactive compounds inducing ER stress-mediated apoptosis: A review. Nutrients, 10(8): 1021. doi: 10.3390/nu10081021.
Kocabas YZ, Ilcim A, 2016. Anatomical features of Dorycnium Miller. (Fabaceae) species growing in Turkey. Karaelmas Sci Eng J, 6(1): 83-89.
Kumar RS, Murugananthan G, Rajkapoor B, 2017. Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth. (Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats. Braz J Biol Sci, 4(7): 53-66.
Lacerda LP, Malaquias G, Peron AP, 2014. Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L. An Acad Brasil Cienc, 86(3): 1147-1150.
Lopes FC, Calvo TR, Colombo LL, Vilegas W, Carlos IZ, 2011. Immunostimulatory and cytotoxic activities of Indigofera suffruticosa (Fabaceae). Nat Prod Res, 25(19): 1796-1806.
Martin-Cordero C, Leon-Gonzalez AJ, Calderon-Montano JM, Burgos-Moron E, Lopez-Lazaro M, 2012. Pro-oxidant natural products as anticancer agents. Curr Drug Targets, 13(8): 1006-1028.
Mentese U, Dogan OV, Turan I, Usta S, Dogan E, Oztas Mentese S, Demir S, Ozer T, Aykan AC, Alver A, 2014. Oxidant-antioxidant balance during on-pump coronary artery bypass grafting. Scientific World Journal, Article ID 263058, doi: 10.1155/2014/263058.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71(1-2): 109-114.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55-63.
Noufou O, Sawadogo WR, Tibiri A, Lompo M, Dijoux MG, Diederich M, Guissou IP, 2016. Phenolic contents and in vitro pharmacological activities of methanolic extract of Pterocarpus erinaceus Poir. stem bark (Fabaceae). BJPR, 10(2): 1-7.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y, 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod, 12(1): e40073, doi: 10.5812/jjnpp.40073.
Phani Kumar K, Shyam Prasad K, Lakshmi Sudeepthi N, Ravi Chandra SRD, Abdul Rahaman S, Madan Ranjith P, 2014. In vitro cytotoxic activity of aqueous extract of Delonix elata (L.) gamble (Fabaceae) leaves on MCF-7 and HepG2 cell lines. IJPT, 2(2): 70-75.
Rathi SG, Suthar M, Patel P, Bhaskar VH, Rajgor NB, 2009. In-vitro cytotoxic screening of Glycyrrhiza glabra L. (Fabaceae): a natural anticancer drug. J Young Pharm, 1(3): 239-243.
Ravishankar D, Rajora AK, Greco F, Osborn HM, 2013. Flavonoids as prospective compounds for anti-cancer therapy. Int J Biochem Cell Biol, 45(12): 2821-2831.
Slinkard K, Singleton VL, 1977. Total phenol analyses: automation and comparison with manual methods. Am J Enol Viticult, 28: 49-55.
Sobeh M, El-Hawary E, Peixoto H, Labib RM, Handoussa H, Swilam N, El-Khatib AH, Sharapov F, Mohamed T, Krstin S, Linscheid MW, Singab AN, Wink M, Ayoub N, 2016. Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans. Peer J, 4: e2404, doi: 10.7717/peerj.2404.
Stefanović OD, Tešić JD, Čomić LR, 2015. Melilotus albus and Dorycnium herbaceum extracts as source of phenolic compounds and their antimicrobial, antibiofilm, and antioxidant potentials. J Food Drug Anal, 23(3): 417-424.
Teyeb H, Zanina N, Neffati M, Douki W, Najjar MF, 2012. Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia. Turk J Biol, 36: 53-58.
Turan I, Deger O, Aliyazicioglu Y, Demir S, Kilinc K, Sumer A, 2015. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1. Turk J Med Sci, 45(4): 804-811.
Turan I, Demir S, Kilinc K, Arslan Burnaz N, Ozer Yaman S, Akbulut K, Mentese A, Aliyazicioglu Y, Deger O, 2017a. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells. Saudi Phar J, 25(2): 241-248.
Turan I, Demir S, Kilinc K, Aliyazicioglu Y, Alver A, Misir S, Ozer Yaman S, Akbulut K, Mentese A, Deger O, 2017b. Morus rubra extract induces G1 cell cycle arrest and apoptosis in human lung and prostate cancer cells. IJPER, 51(1): 51-58.
Usta C, Yıldırım AB, Turker AU 2014. Antibacterial and antitumour activities of some plants grown in Turkey. Biotechnol Biotechnol Equip, 28(2): 306-315.
Yalcin CO, Aliyazicioglu Y, Demir S, Turan I, Bahat Z, Misir S, Deger O, 2016. Evaluation of radioprotective effect of Turkish propolis on foreskin fibroblast cells. J Cancer Res Ther, 12(2): 990-994.
Zhang J, Nishimoto Y, Tokuda H, Suzuki N, Yasukawa K, Kitdamrongtham W, Akazawa H, Manosroi A, Manosroi J, Akihisa T, 2013. Cancer chemopreventive effect of bergenin from Peltophorum pterocarpum wood. Chem Biodivers, 10(10): 1866-1875.
Zingue S, Tchoumtchoua J, Ntsa DM, Sandjo LP, Cisilotto J, Nde CBM, Winter E, Awounfack CF, Ndinteh DT, Clyne C, Njamen D, Halabalaki M, Creczynski-Pasa TB, 2016. Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms. BMC Complement Altern Med, 16(1): 421.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Selim Demir ^*
0000-0002-1863-6280
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Sema Mısır
Türkiye

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Publication Date

June 30, 2019

Submission Date

December 15, 2018

Acceptance Date

February 4, 2019

Published in Issue

Year 2019 Volume: 22 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.497868

IZ

https://izlik.org/JA36UG28MN

Cite

RIS / Bibtex

APA

Demir, S., Turan, İ., Mısır, S., & Aliyazıcıoğlu, Y. (2019). Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(3), 473-479. https://doi.org/10.18016/ksutarimdoga.vi.497868

Cited By

Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

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https://doi.org/10.3390/biom11050671