Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Selim Demir; İbrahim Turan; Sema Mısır; Yüksel Aliyazıcıoğlu

doi:10.18016/ksutarimdoga.vi.497868

EN TR

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Abstract

Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3±1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC₅₀ values of the extract in the cancer cells range from 100.4 to 298.5 μg/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.

Keywords

Dorycnium pentaphyllum Ekstraktının İnsan Meme, Karaciğer ve Akciğer Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Öz

Fabaceae ailesine mensup bitkilerin sitotoksik etkilerini konu alan bazı çalışmalar bulunmakla birlikte, Dorycnium pentaphyllum'un sitotoksik etkisi ile ilgili sınırlı araştırma bulunmaktadır. Bu çalışmanın amacı, D. pentaphyllum ekstraktının antioksidan özelliklerinin ve çeşitli insan kanser hücreleri üzerindeki sitotoksik etkisini belirlenmesidir. Ekstraktın toplam fenolik içeriği kolorimetrik yöntem kullanılarak belirlendi. Ekstraktın insan akciğer (A549), karaciğer (HepG2) ve meme (MCF-7) kanser hücreleri ile normal insan fibroblast hücreleri üzerindeki sitotoksik etkisi MTT analizi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içerik değeri g örnek başına 140.3±1.1 mg gallik asit eşdeğeri olarak bulundu. Ekstraktın çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdiği belirlendi ve IC₅₀ değerleri 100.4-298.5 μg/mL arasında hesaplandı. Bu çalışma D. pentaphyllum ekstraktının kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. D. pentaphyllum'un fitokimyasal uygulamaları kanser tedavisinde umut verici yaklaşımları temsil edebilir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Selim Demir ^*
0000-0002-1863-6280
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Sema Mısır
Türkiye

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Yayımlanma Tarihi

30 Haziran 2019

Gönderilme Tarihi

15 Aralık 2018

Kabul Tarihi

4 Şubat 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 22 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.497868

IZ

https://izlik.org/JA36UG28MN

Kaynak Göster

RIS / Bibtex

APA

Demir, S., Turan, İ., Mısır, S., & Aliyazıcıoğlu, Y. (2019). Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22(3), 473-479. https://doi.org/10.18016/ksutarimdoga.vi.497868

Cited By

Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

Biomolecules

https://doi.org/10.3390/biom11050671

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

Abstract

Keywords

Dorycnium pentaphyllum Ekstraktının İnsan Meme, Karaciğer ve Akciğer Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

e-ISSN: 2619-9149