Year 2019, Volume 22 , Issue 3, Pages 451 - 455 2019-06-30

Comparison of Conventional PCR Method With Cefoxitin Disc Diffusion, Automated System and Isolation on Chromogenic MRSA Medium Methods for The Detection of Meticillin Resistance In Staphylococcus aureus Strains Isolated From Various Clinical Specimens
Çeşitli Klinik Örneklerden Izole Edilen Staphylococcus aureus Suşlarında Metisilin Direncinin Araştırılmasında Konvansiyonel PCR Yöntemi ile Sefoksitin Disk Difüzyon, Otomatize Sistem ve Chromogenic MRSA Agar Yöntemlerinin Karşılaştırılması

Esra KAYA [1] , Murat ARAL [2] , Zerife ORHAN [3] , Kezban Tülay YALÇINKAYA [4] , Hacer UĞURLU [5]


Meticillin-resistant Staphylococcus aureus (MRSA) is one of the most important infectious agents. Therefore, fast and accurate diagnosis of MRSA is utmost important. Although mecA gene detection by PCR is reference method, conventional methods are preferred in routine practices due to simplicity. As conventional methods last 48-96 hours, several chromogenic media have been developed. Our objective was to compare the methods used for meticillin resistance detection with PCR. Forty-eight S. aureus strains isolated from various clinical specimens were included. Of the 48 S. aureus strains, 19 were mecA gene-positive and 29 were negative. mecA gene-positive 19 strains were also meticillin-resistant by automated system and disk diffusion. On chromogenic agar, 15 of 19 MRSA strains were meticillin-resistant and 4 were meticillin-sensitive. Twentynine mecA gene-negative strains were susceptible to meticillin by automatized system and disk diffusion. Among 29 mecA gene-negative MSSA strains inoculated on chromogenic agar, 17 were methicillin-resistant. According to our study, chromogenic media would be ineffective to detected to meticillin-resistance because of low sensitivity and specificity in routine.

Metisiline dirençli Staphylococcus aureus (MRSA) en önemli enfeksiyöz ajanlardan biridir. Bu nedenle MRSA'nın hızlı ve doğru tanısı çok önemlidir. PCR ile mecA gen tespiti referans metodu olmakla birlikte, basit olmasından dolayı rutin uygulamada geleneksel yöntemler tercih edilmektedir. Geleneksel yöntemler ile tanının koyulması 48-96 saat sürdüğü için çeşitli kromojenik besiyerleri geliştirilmiştir. Çalışmamızdaki amacımız metisilin direncinin tespitinde kullanılan çeşitli yöntemleri PCR ile karşılaştırmaktır. Çeşitli klinik örneklerden izole edilen 48 S. aureus suşu çalışmamıza dahil edilmiştir.48 S.aureus suşunun 19'u mecA gen-pozitif ve 29'u negatiftir. mecA gen-pozitif 19 suş da otomatik sistem ve disk difüzyonu ile dirençli bulunmuştur.  Kromojenik agarda, 19 MRSA suşunun 15'i metisilin dirençli ve 4'ü duyarlı bulunmuştur.29 mecA gen-negatif suş, otomatik sistem ve disk difüzyonu ile duyarlı bulunmuştur. mecA geni negatif olan 29 MSSA suşunun kromojenik agarda 17’si dirençli olarak değerlendirmiştir.

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Primary Language en
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-0732-6471
Author: Esra KAYA (Primary Author)
Country: Turkey


Orcid: 0000-0002-3576-4380
Author: Murat ARAL

Orcid: 0000-0003-2154-3074
Author: Zerife ORHAN

Orcid: 0000-0002-6324-4585
Author: Kezban Tülay YALÇINKAYA

Orcid: 0000-0001-6126-5502
Author: Hacer UĞURLU

Dates

Application Date : September 5, 2018
Acceptance Date : January 30, 2019
Publication Date : June 30, 2019

APA Kaya, E , Aral, M , Orhan, Z , Yalçınkaya, K , Uğurlu, H . (2019). Comparison of Conventional PCR Method With Cefoxitin Disc Diffusion, Automated System and Isolation on Chromogenic MRSA Medium Methods for The Detection of Meticillin Resistance In Staphylococcus aureus Strains Isolated From Various Clinical Specimens . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (3) , 451-455 . DOI: 10.18016/ksutarimdoga.vi.457495