Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep
Abstract
Yeasts are usually found in high amounts in dairy
products, which show their ability to adapt to substrates rich in protein,
lipid, sugar and organic acids. A wide distribution of yeast in dairy products
is a result of proteolytic and lipolytic activities. Spoilage yeasts and molds
can grow in most processed and raw foods, where environmental conditions are
not suitable for most bacteria (low pH, low water activity, aw). Nutrients and
oxygen in food are the main factors that determine the type of fungal spoilage.
In this study, dairy products samples (yoghurt, cream, butter, curd cheese,
Antep cheese) were collected from local
markets in Gaziantep province. In our study, a total of 20 yeast strains were
isolated from dairy products and investigated for lipase activities in solid
media containing tributyrin. Twenty yeast isolates identified by
amplification and sequencing of the ITS region using ITS1 and ITS4 primers Yeasts were identified as Kluyveromyces marxianus (8 isolates), Candida intermedia (8 isolates), Pichia fermentans (2 isolates), Yarrowia lipolytica (1 isolate), Kluyveromyces lactis (1 isolate).
Supporting Institution
Gaziantep University
Project Number
(FEF. 15.03)
Thanks
This work was supported by the Scientific Research Unit of Gaziantep University (FEF. 15.03). We would like to sincerely thank the Scientific Research Unit of Gaziantep University for providing funding and necessary logistics for this research work
References
- Fleet GH 1990. Yeasts in dairy products. Journal of Applied Bacteriology, 68: 199-211.
- Rohm H, Eliskasses F, Bräuer M 1992. Diversity of yeasts in selected dairy products. Journal of Applied Bacteriology, 72: 370–376.
- Fox CW, Chrisope GL, Marshall RT 1976. Incidence and identification of phospholipase C-producing bacteria in fresh and spoiled homogenized milk. Journal of Dairy Science, 59:1857–64.
- Verger R 1997. Interfacial activation of lipases: facts and artefacts. Trends in Biotechnology, 15: 32-8.
- Schmid RD, Verger R 1998. Lipases: Interfacial Enzymes with Attractive Applications. Angew Chem Int Ed Engl. 3;37(12):1608-33.
- Prakash D, Nawani N, Prakash M, Bodas, M, Mandal A, Khetmalas M, Kapadnis B 2013. Actinomycetes: A repertory of green catalysts with a potential revenue resource. Biomed. Res. Int. 2013: 1–8.
- Sanchez S, Demain AL 2011. Enzymes and bioconversions of industrial, pharmaceutical, and biotechnological significance, Organic Process Research & Development. 15(1): 224-30.
- Singhania RR, Patel AK, Pandey A 2010. The Industrial Production of Enzymes. In Industrial Biotechnology (eds W. Soetaert and E. J. Vandamme). 2010: 207-25.
- Lin SK, Seelbach K, Wandrey C 2001. Industrial Biotransformations. Molecules, 6: 1044-6.
- Adrio JL, Demain AL 2014. Microbial enzymes: tools for biotechnological processes. Biomolecules, 16;4(1):117-39.
- Demain AL, Adrio JL 2008. Contributions of microorganisms to industrial biology. Mol Biotechnol, 38(1):41-55.
- Yalcin HT, Ucar FB 2009. Isolation and characterization of cheese spoiler yeast isolated from Turkish white cheeses. Ann Microbiol, 59(3):477-483.
- Corbaci C, Ucar FB, Yalcin HT 2012. Isolation and characterization of yeasts associated with Turkish-style homemade dairy products and their potential as starter cultures. Afr J Microbiol Res, 6(3):534-542.
- Liu D, Coloe S, Baird R, Pederson J 2000. Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol, 38(1):471.
- Tamura K, Nei M 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol, 10(3):512-26.
- Kumar S, Stecher G, Tamura K 2016 MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol, 33(7):1870-4.
- Limtong S, Kaewwichian R, Jindamorakot S, Yongmanitchai W, Nakase T 2012. Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand. Antonie Van Leeuwenhoek, 102(1):23-8.
- Shah, NP, 1994. Psychrotrophs in milk: a review. Milchwissenschaft, 49(8): 432–7.
- Sorhaug T, Stepaniak JL, 1997. A review: psychrotrophs and their enzymes in milk and dairy products Trends Food Sci. Technol, 8: 35-41
- Johnson EA, Nelson JH, Johnson M 1990. Microbiological safety of cheese made from heat-treated milk, part III. Technology, discussion, recommendations, bibliography Journal of Food Protection, 53(7): 610-23
- Suriyarachchi VR, Fleet GH 1981. Occurrence and growth of yeasts in yogurts. Appl Environ Microbiol 42: 574–9.
- Kosse D, Seiler H, Amann R, Ludwig W, Scherer S 1997. Identification of yoghurtspoiling yeasts with 18S rRNA-targeted oligonucleotide probes. Syst. Appl. Microbiol, 20(3): 468-80.
- Sagdic O, Ozturk I, Bayram O, Kesmen Z, Yilmaz MT 2010. Characterization of Butter Spoiling Yeasts and Their Inhibition by Some Spices. Journal of Food Science, 75(9):M597-603.
- Lopandic K, Zelger S, Banszky LK, Eliskases-Lechner F, Prillinger H. 2006. Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiol 23:341–50
- Thakur S 2012 Lipases, its sources, properties and applications: a review. Int J Sci Eng Res, 3:1–29.
- Vakhlu J, Kour A 2006. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol, 9: 70–85.
- Palekar AA, Vasudevan PT, Yan S 2000. Purification of lipase: a review. Biocatal Biotransform, 3:177–200.
- Ciafardini G, Zullo BA, Iride A 2006.Lipase production by yeasts from extra virgin olive oil. Food Microbiol, 23:60–7.
- Potumarthi P, Subhakar C, Vanajakshi J, Jetty A 2008. Effect of aeration and agitation regimes on lipase production by newly isolated Rhodotorula mucilaginosa-MTCC 8737 in stirred tank reactor using molasses as sole carbon source. Appl Biochem Biotechnol, 151:700–10.
- Lukaszewicz M, Jablonski S, Krasowska A. Characterization of alkaline lipase from an arctic yeast strain Rhodosporidium babjevae BD19. Eur Sci J, 2013:1480–9.
- Çorbaci C, Uyar E, Yalçin HT 2017. Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples. Celal Bayar Universty Journal of Science. 13(4):833-7
Gaziantep İline Ait Bazı Süt Ürünlerinde Bozulmaya Neden Olan Mayalardan Ekstraselüler Lipaz Enzimi Aranması ve Lipaz Aktivitesine Sahip Suşların Genotipik İdentifikasyonu
Abstract
Mayalar genellikle süt ürünlerinde yüksek miktarda bulunurlar; bu da
onların protein, lipid, şeker ve organik asitlerce zengin substratlara iyi bir
şekilde adapte olabilme yeteneklerini gösterir. Bozulmaya neden olan mayalar ve
küfler, çoğu bakteri için çevresel koşulların uygun olmadığı (düşük pH, düşük
su aktivitesi, aw) işlenmiş ve çiğ gıdalarda gelişim gösterebilmektedir.
Mayaların süt ürünlerinde geniş bir dağılıma sahip olmaları proteolitik ve
lipolitik aktivitelerinin bir sonucudur. Besin maddeleri ve oksijen gıdalardaki
fungal bozulma tipini belirleyen ana faktörlerdir. Bu çalışmada Gaziantep
ilindeki yerel marketlerden çeşitli süt ürünleri örnekleri (yoğurt, krema,
tereyağı, lor peyniri, Antep peyniri) alındı. Toplam 20 maya suşu izole edildi
ve tributirinden oluşan katı ortamdaki lipaz aktiviteleri açısından
araştırıldı. ITS1 ve ITS4 primerleri kullanılarak ITS bölgesinin amplifikasyonu
ve sekanslanması ile mayalardan 8 izolat Kluyveromyces
marxianus, 8 izolat Candida
intermedia, 2 izolat Pichia
fermentans, 1 izolat Yarrowia
lipolytica, 1 izolat Kluvyeromyces
lactis olarak tanımlanmıştır.
Project Number
(FEF. 15.03)
References
- Fleet GH 1990. Yeasts in dairy products. Journal of Applied Bacteriology, 68: 199-211.
- Rohm H, Eliskasses F, Bräuer M 1992. Diversity of yeasts in selected dairy products. Journal of Applied Bacteriology, 72: 370–376.
- Fox CW, Chrisope GL, Marshall RT 1976. Incidence and identification of phospholipase C-producing bacteria in fresh and spoiled homogenized milk. Journal of Dairy Science, 59:1857–64.
- Verger R 1997. Interfacial activation of lipases: facts and artefacts. Trends in Biotechnology, 15: 32-8.
- Schmid RD, Verger R 1998. Lipases: Interfacial Enzymes with Attractive Applications. Angew Chem Int Ed Engl. 3;37(12):1608-33.
- Prakash D, Nawani N, Prakash M, Bodas, M, Mandal A, Khetmalas M, Kapadnis B 2013. Actinomycetes: A repertory of green catalysts with a potential revenue resource. Biomed. Res. Int. 2013: 1–8.
- Sanchez S, Demain AL 2011. Enzymes and bioconversions of industrial, pharmaceutical, and biotechnological significance, Organic Process Research & Development. 15(1): 224-30.
- Singhania RR, Patel AK, Pandey A 2010. The Industrial Production of Enzymes. In Industrial Biotechnology (eds W. Soetaert and E. J. Vandamme). 2010: 207-25.
- Lin SK, Seelbach K, Wandrey C 2001. Industrial Biotransformations. Molecules, 6: 1044-6.
- Adrio JL, Demain AL 2014. Microbial enzymes: tools for biotechnological processes. Biomolecules, 16;4(1):117-39.
- Demain AL, Adrio JL 2008. Contributions of microorganisms to industrial biology. Mol Biotechnol, 38(1):41-55.
- Yalcin HT, Ucar FB 2009. Isolation and characterization of cheese spoiler yeast isolated from Turkish white cheeses. Ann Microbiol, 59(3):477-483.
- Corbaci C, Ucar FB, Yalcin HT 2012. Isolation and characterization of yeasts associated with Turkish-style homemade dairy products and their potential as starter cultures. Afr J Microbiol Res, 6(3):534-542.
- Liu D, Coloe S, Baird R, Pederson J 2000. Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol, 38(1):471.
- Tamura K, Nei M 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol, 10(3):512-26.
- Kumar S, Stecher G, Tamura K 2016 MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol, 33(7):1870-4.
- Limtong S, Kaewwichian R, Jindamorakot S, Yongmanitchai W, Nakase T 2012. Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand. Antonie Van Leeuwenhoek, 102(1):23-8.
- Shah, NP, 1994. Psychrotrophs in milk: a review. Milchwissenschaft, 49(8): 432–7.
- Sorhaug T, Stepaniak JL, 1997. A review: psychrotrophs and their enzymes in milk and dairy products Trends Food Sci. Technol, 8: 35-41
- Johnson EA, Nelson JH, Johnson M 1990. Microbiological safety of cheese made from heat-treated milk, part III. Technology, discussion, recommendations, bibliography Journal of Food Protection, 53(7): 610-23
- Suriyarachchi VR, Fleet GH 1981. Occurrence and growth of yeasts in yogurts. Appl Environ Microbiol 42: 574–9.
- Kosse D, Seiler H, Amann R, Ludwig W, Scherer S 1997. Identification of yoghurtspoiling yeasts with 18S rRNA-targeted oligonucleotide probes. Syst. Appl. Microbiol, 20(3): 468-80.
- Sagdic O, Ozturk I, Bayram O, Kesmen Z, Yilmaz MT 2010. Characterization of Butter Spoiling Yeasts and Their Inhibition by Some Spices. Journal of Food Science, 75(9):M597-603.
- Lopandic K, Zelger S, Banszky LK, Eliskases-Lechner F, Prillinger H. 2006. Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiol 23:341–50
- Thakur S 2012 Lipases, its sources, properties and applications: a review. Int J Sci Eng Res, 3:1–29.
- Vakhlu J, Kour A 2006. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol, 9: 70–85.
- Palekar AA, Vasudevan PT, Yan S 2000. Purification of lipase: a review. Biocatal Biotransform, 3:177–200.
- Ciafardini G, Zullo BA, Iride A 2006.Lipase production by yeasts from extra virgin olive oil. Food Microbiol, 23:60–7.
- Potumarthi P, Subhakar C, Vanajakshi J, Jetty A 2008. Effect of aeration and agitation regimes on lipase production by newly isolated Rhodotorula mucilaginosa-MTCC 8737 in stirred tank reactor using molasses as sole carbon source. Appl Biochem Biotechnol, 151:700–10.
- Lukaszewicz M, Jablonski S, Krasowska A. Characterization of alkaline lipase from an arctic yeast strain Rhodosporidium babjevae BD19. Eur Sci J, 2013:1480–9.
- Çorbaci C, Uyar E, Yalçin HT 2017. Determination of Enzyme Profiles and Molecular Characterization of Yeast Species Isolated from Butter Samples. Celal Bayar Universty Journal of Science. 13(4):833-7
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | (FEF. 15.03) |
| Publication Date | October 31, 2019 |
| Submission Date | April 18, 2019 |
| Acceptance Date | May 31, 2019 |
| Published in Issue | Year 2019Vol 22 (Suppl 1) |
Cite
Cited By
Occurrence of Yeasts in White-Brined Cheeses: Methodologies for Identification, Spoilage Potential and Good Manufacturing Practices
Frontiers in Microbiology
Athina Geronikou
https://doi.org/10.3389/fmicb.2020.582778
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