Bakır Oksit Nanopartikülü Etkisinde Kalan Memelilerde (Rattus norvegicus var. albinos) Bazı Metabolik Tepkilerin Incelenmesi

Esin Gülnaz Canlı

doi:10.18016/ksutarimdoga.vi.632772

EN TR

Bakır Oksit Nanopartikülü Etkisinde Kalan Memelilerde (Rattus norvegicus var. albinos) Bazı Metabolik Tepkilerin Incelenmesi

Öz

Bu çalışmada, dişi sıçanlara 14 gün boyunca oral gavaj ile farklı dozlarda (kontrol (0), 0.5, 5, 50 mg/kg/gün) CuO nanopartikülü (NP) verildi. Bunu takiben böbrek, beyin ve ince bağırsak dokularında ATPaz aktiviteleri ve beyin dokusunda asetilkolinesteraz (AChE) aktivitesi ölçüldü. Ayrıca, karaciğerde farklı glutatyon formları (toplam GSH, rGSH, GGSG) ve lipit peroksidasyonu ürünü olan TBARS (tiyobarbitürik asit reaktif maddeleri) düzeyleri de ölçüldü. Diğer yandan, dokularda CuO NP birikimi bir transmisyon elektron mikroskobu (TEM) yardımıyla görüntülendi. Sonuçlar, beyin AChE aktivitesinde anlamlı (P<0.05) azalmalar olduğunu göstermiştir. Beyin ve ince bağırsak ATPaz aktiviteleri istatistiksel olarak anlamlı değişimler göstermezken (P>0.05), böbrek ATPaz aktivitesinde istatiksel olarak (P˂0.05) anlamlı azalışlar olmuştur. Farklı glutatyon formlarının düzeyleri en yüksek doz haricinde anlamlı bir şekilde değişmemiştir. Benzer şekilde, TBARS düzeyleri sadece en yüksek dozda istatiksel olarak anlamlı (P<0.05) artışlar göstermiştir. TEM görüntüleri CuO NP'lerin sıçan dokularda birikebileceğini göstermiş olup, bu görüntüler sıçanlarda enzimatik ve enzimatik olmayan biyobelirteçlerde meydana gelen değişikliklerin dokularda CuO NP birikmesinden kaynaklandığını vurgulamıştır.

Anahtar Kelimeler

Metal,Nanopartikül,Memeli,Biyomarkır,TEM,Bakır oksit

Destekleyen Kurum

Çukurova Üniversitesi

Proje Numarası

FDK-2017-8197

Teşekkür

Bu çalışmanın verilerinin bir kısmı Çukurova Üniversitesi Araştırma Fonu tarafından desteklenen projeye (FDK-2017-8197) aittir. Fizikçi hocam Prof. Dr. Cebrail Gümüş’e nanopartikül karakterizasyonu konusunda ve Doktora Tez Danışmanım Prof. Dr. Hasan. B. İla’ya desteklerinden dolayı çok teşekkür ederim.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Esin Gülnaz Canlı
0000-0002-0132-3712
Türkiye

Yayımlanma Tarihi

30 Nisan 2020

Gönderilme Tarihi

14 Ekim 2019

Kabul Tarihi

5 Aralık 2019

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.632772

IZ

https://izlik.org/JA35AJ66EP

Kaynak Göster

RIS / Bibtex

APA

Canlı, E. G. (2020). Bakır Oksit Nanopartikülü Etkisinde Kalan Memelilerde (Rattus norvegicus var. albinos) Bazı Metabolik Tepkilerin Incelenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(2), 304-315. https://doi.org/10.18016/ksutarimdoga.vi.632772

Cited By

METAL OKSİT NANOPARTİKÜLLERİN GENOTOKSİK ETKİLERİ

International Journal of Advances in Engineering and Pure Sciences

https://doi.org/10.7240/jeps.875709

Bakır Oksit Nanopartikülü Etkisinde Kalan Memelilerde (Rattus norvegicus var. albinos) Bazı Metabolik Tepkilerin Incelenmesi

Investigation of Some Metabolic Responses in Mammals (Rattus norvegicus var. albinos) Exposed to Copper Oxide Nanoparticles

Abstract

Keywords

Bakır Oksit Nanopartikülü Etkisinde Kalan Memelilerde (Rattus norvegicus var. albinos) Bazı Metabolik Tepkilerin Incelenmesi

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

METAL OKSİT NANOPARTİKÜLLERİN GENOTOKSİK ETKİLERİ

e-ISSN: 2619-9149