Benchmarking of the Effects of Salinity on Antioxidant Enzymes Activities, Lipid Peroxidation and H2O2 Levels in the Leaves of Two Zinnia Species

Sara Yasemin; Ayşin Güzel Değer; Sertan Çevik; Nezihe Köksal

doi:10.18016/ksutarimdoga.vi.741890

TR EN

İki Zinnia Türünün Yapraklarında Tuzluluğun Antioksidan Enzim Aktiviteleri, Lipid Peroksidasyonu ve H2O2 Düzeyleri Üzerine Etkilerinin Karşılaştırılması

Öz

Bu çalışmada, önemli bir çevresel sorun olan tuzluluğun, süs bitkilerinin (zinnia gibi) yetiştirilmesinde ve yüksek tuzlu su ile sulamada, özellikle antioksidan savunma mekanizması üzerindeki etkilerinin araştırılması amaçlanmıştır. Bu amaçla, iki Zinnia türü farklı konsantrasyonlarda tuzlu su (50, 100, 150, 200 mM NaCl) ile sulanmıştır; tuzluluğun yapraklardaki süperoksit dismutaz (SOD), katalaz (CAT), glutatyon redüktaz (GR) lipit peroksidasyonu (MDA) ve hidrojen peroksit (H2O2) üzerindeki etkileri belirlenmiştir. Sonuçlar, tuzluluğun iki Zinnia türünde SOD, CAT, GR, H2O2 ve MDA içeriğini kontrol gruplarına kıyasla belirgin şekilde arttırdığını göstermiştir. SOD ve CAT enzim aktivitelerinin her iki Zinnia türünde 150 mM NaCl ile önemli ölçüde arttığı, ancak 200 mM NaCl ile azaldığı bulunmuştur. En yüksek GR enzim aktivitesi, Zinnia marylandica "Double Zahara Fire Improved" da 200mM tuz konsantrasyonunda gözlenmiştir. MDA ve H2O2 seviyeleri Zinnia elegans 'Zinnita Scarlet' da daha yüksek olarak gözlemlenmiştir. Sonuç olarak, bu iki Zinnia çeşidinin 150 mM'a kadar tuz konsantrasyonunu tolere edebildiği söylenebilir.

Anahtar Kelimeler

Benchmarking of the Effects of Salinity on Antioxidant Enzymes Activities, Lipid Peroxidation and H2O2 Levels in the Leaves of Two Zinnia Species

Abstract

In this study, it was aimed to investigate the effects of salinity, which is an important environmental problem, in the cultivation of ornamental plants (such as zinnia) and irrigation with high salt water, especially on the antioxidant defense mechanism. For this purpose, the two Zinnia species were irrigated by different concentrations of saline water (50, 100, 150, 200 mM NaCl); effects of salinity on superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) lipid peroxidation (MDA) and hydrogen peroxide (H2O2) in the leaves were determined. The results showed that salinity conspicuously increased SOD, CAT, GR, H2O2 and MDA content at two Zinnia species compared to the control groups. It was found that SOD and CAT enzyme activities increased remarkably with 150 mM NaCl in both Zinnia species, but decreased with 200 mM NaCl.The highest GR enzyme activity was observed in 200mM salt concentration at Zinnia marylandica ‘Double Zahara Fire Improved’. MDA and H2O2 levels were observed higher in Zinnia elegans ‘Zinnita Scarlet’.To conclude; it may be said that these two Zinnia varieties can tolerate salt concentration up to 150 mM.

Keywords

Destekleyen Kurum

Çukurova University

Proje Numarası

Project No: FBA-2019-11481.

Teşekkür

This work was supported Çukurova University, Project No: FBA-2019-11481. We thank the Prof.Dr.Serpil ÜNYAYAR, Girne American University/Turkish Republic of Northern Cyprus, for supports.

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji , Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Sara Yasemin
0000-0003-2193-6791
Türkiye

Ayşin Güzel Değer ^*
0000-0001-6336-1872
Türkiye

Sertan Çevik
0000-0003-1259-7863
Türkiye

Nezihe Köksal
0000-0002-5401-9730
Türkiye

Yayımlanma Tarihi

28 Şubat 2021

Gönderilme Tarihi

26 Mayıs 2020

Kabul Tarihi

9 Temmuz 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.741890

IZ

https://izlik.org/JA28HD28KU

Kaynak Göster

RIS / Bibtex

APA

Yasemin, S., Güzel Değer, A., Çevik, S., & Köksal, N. (2021). Benchmarking of the Effects of Salinity on Antioxidant Enzymes Activities, Lipid Peroxidation and H2O2 Levels in the Leaves of Two Zinnia Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(1), 31-39. https://doi.org/10.18016/ksutarimdoga.vi.741890