Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

Mehmet Göse; Nurcihan Hacıoğlu

doi:10.18016/ksutarimdoga.v24i60916.750034

TR EN

İki Verbascum Türünün Biyoaktif Bileşenleri, Antimikrobiyal ve Antibiyofilm Aktiviteleri

Öz

Verbascum pinnatifidum Vahl. ve V. antinori Boiss. et Heldr’den elde edilen methanol, aseton ve etil asetat ekstraktları bioaktif bileşenleri, antimikrobiyal ve antibiyofilm aktiviteleri bakımından araştırıldı. Antimikrobiyal ve antibiyofilm aktiviteleri Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Staphylococcus haemolyticus ATCC 43252 ve Candida albicans ATCC 10231 ile sırasıyla disk difüzyon, minimum inhibitör konsantrasyonu ve minimum bakterisidal veya fungisidal konsantrasyonu ve mikroplak biyofilm metodu ile belirlendi. Biyoaktif bileşik analizleri kumarinler, kardiak glikozidler, kuinonlar ve flavanonların varlığını ortaya koymuştur. V. antinori ekstraktlarının test mikroorganizmalarına karşı V. pinnatifidum ekstraktlarından daha yüksek antimikrobiyal aktivite sahip olduğu ortaya çıkmıştır. En düşük MIK değeri A. baumanii ATCC 19606 (0.3125 ± 0.01)’ya karşı V. pinnatifidum methanol ekstraktı ile kaydedilmiştir. Üç V. pinnatifidum ve V. antinori ekstraktının antibiyofilm aktiviteleri sırasıyla %8.93 - 92.18 ve %14.56 - 91.19 oranlarında inhibisyon yüzdesi göstermiştir.

Anahtar Kelimeler

Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

Abstract

Methanol, acetone and ethyl acetate extracts obtained from Verbascum pinnatifidum Vahl. and V. antinori Boiss. et Heldr were investigated for their bioactive composition, antimicrobial and antibiofilm activity. Antimicrobial and antibiofilm activity was determined with Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Staphylococcus haemolyticus ATCC 43252 and Candida albicans ATCC 10231 by the disk diffusion, minimum inhibitory concentration and minimum bactericidal or fungicidal concentration and microplate biofilm method, respectively. Bioactive compounds analyses reveal the presence of coumarins, cardiac glycosides, quinones, flavanones. It was revealed that extracts of V. antinori has more antimicrobial activity than V. pinnatifidum extracts against test microorganisms. The lowest MIC value was recorded by V. pinnatifidum methanol extract against A. baumanii ATCC 19606 (0.3125 ± 0.01). Antibiofilm activities of three extracts of V. pinnatifidum and V. antinori have been showed an inhibition percentage range of 8.93 - 92.18% and 14.56 - 91.19%, respectively.

Keywords

Destekleyen Kurum

Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey

Proje Numarası

FYL-2018-2693

Teşekkür

This investigation is a part of Master thesis of Mehmet GÖSE. This study was financially supported by the Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey (FYL-2018-2693). Authors would like to thank Assoc. Prof. Dr. Ersin KARABACAK and Assoc. Prof. Dr. Neslihan DEMİR for defining plant species and helping phytochemical screening, respectively.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Mehmet Göse
0000-0002-6029-2442
Türkiye

Nurcihan Hacıoğlu ^*
0000-0002-5812-9398
Türkiye

Yayımlanma Tarihi

30 Haziran 2021

Gönderilme Tarihi

9 Haziran 2020

Kabul Tarihi

30 Eylül 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.v24i60916.750034

IZ

https://izlik.org/JA73CP99GE

Kaynak Göster

RIS / Bibtex

APA

Göse, M., & Hacıoğlu, N. (2021). Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(3), 479-487. https://doi.org/10.18016/ksutarimdoga.v24i60916.750034

Cited By

Evaluation of anticancerogenic effect of flavonoid rich Verbascum gypsicola Vural & Aydoğdu methanolic extract against SH-SY5Y cell line

Biotech Studies

https://doi.org/10.38042/biotechstudies.1383424

Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

İki Verbascum Türünün Biyoaktif Bileşenleri, Antimikrobiyal ve Antibiyofilm Aktiviteleri

Öz

Anahtar Kelimeler

Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

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e-ISSN: 2619-9149