Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis
Yıl 2020,
Cilt: 23 Sayı: 2, 297 - 303, 30.04.2020
Nigar Saeed
,
Sabahattin Cömertpay
Öz
Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.
Destekleyen Kurum
Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi
Proje Numarası
2017/4-28D
Teşekkür
This work was supported by Scientific Research Projects (BAP) Unit of Kahramanmaras Sutcu Imam University with the project number of 2017/4-28D.
Kaynakça
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
- Gao S, Zhu D, Cai Z, Jiang Y, Xu D 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata. Plant Cell Tissue Organ Culture 59(3):197-201.
- George E, Hall M, De Klerk G 2008. Plant tissue culture procedure- background plant propagation by Tissue culture. Springer, England
- Li S, Li P, Lin G, Chan S, Ho Y 2000. Simultaneous determination of seven major isosteroidal alkaloids in bulb of Fritillaria by gas chromatography. Chromatographia 873(2):221-228.
- Li X, Gao W, Jiang Q, Huang L, Liu C 2011. Study on the morphology crystalline structure, and thermal properties of Fritillaria ussuriensis Maxim starch acetates with different degrees of substitution. Starch-Starke 63(1):24-31.
- Nhut D, Van Le, Fukai S, Tanaka M, Van K 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation 33(1):59-65.
- Perry L, Metzger J 1980. Medicinal plants of East and Southeast Asia: attributed properties. Economic Botany 34(4):361-361.
- Saeed N, Cömertpay S 2017. Activated charcoal improve growth of F. imperialis propagated by indirect organogenesis. Columella. Agriculture Environ., 4:50-64.
- Staikkidou I, Selby C, Hanks G 2000. Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Hortic., 725:421-426.Steinits B, Yahel H 1982. In vitro propagation of Narcissus tazetta. Horticulture Science 17(3):333-334.
- Thomas T 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advance 26(6): 618- 631.
- Wang S, Gao W, Chen H, Xiao P 2005. New starches from Fritillaria species medicinal plants. Carbohydrate polymerases 61(1):111-114.
- Ziv M, Lilien-Kipnis H 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant biology and morphogenesis 19(9):845-850.
6-benzilaminopurin ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi Üzerindeki Etkileri
Yıl 2020,
Cilt: 23 Sayı: 2, 297 - 303, 30.04.2020
Nigar Saeed
,
Sabahattin Cömertpay
Öz
Fritillaria imperialis türü bitkiler, tohumlarını
yenilemek için iki yıldan fazla bir süreye ihtiyacı duymakta ve bu durum
üretimlerini zorlaştırmaktadır. Bu çalışmada, üç farklı derişimdeki
6-Benzilaminopurinin (BAP) (0.00, 0.10 ve 0.15 mg/L) ve üç farklı miktardaki
(0.0, 3.0 ve 3.5 g/L) aktif karbonun (AC) F.
imperialis bitkilerinin büyümesine etkisi in vitro olarak
incelenmiştir. Tüm muamele grupları için benzer konsantrasyonda oksin (0.01
mg/L NAA ve 0.01 mg/L IAA) kullanılmıştır. 0.10 mg/L BAP ilave edilmiş kültür
ortamlarına 3.5 g/L AC eklenmesinin bitkilerin yumru çap uzunluğu ve sayısının
yanında kök ve sürgün sayılarını istatiksel olarak anlamlı bir şekilde (p<0.05) arttırdığı
gözlenmiştir. Dahası, AC eklenmesi, çalışılan tüm dozlarda kök uzunluklarını
önemli ölçüde azaltmıştır (p<0.05). Yüksek BAP konsantrasyonunun (0.15 mg/L)
çalışılan tüm grupların yumru sayılarını azalttığı, ancak 3.5 g/L AC ile
desteklendiğinde ölçülen tüm değerlerin artışını sağladığı bulunmuştur
(p<0.05). ).
Genel olarak, kallustan bitki kısımlarının en iyi şekilde büyümesi, en yüksek
konsantrasyonda AC ve >1.5 mg/L BAP ile elde
edilmiştir.
Proje Numarası
2017/4-28D
Kaynakça
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
- Gao S, Zhu D, Cai Z, Jiang Y, Xu D 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata. Plant Cell Tissue Organ Culture 59(3):197-201.
- George E, Hall M, De Klerk G 2008. Plant tissue culture procedure- background plant propagation by Tissue culture. Springer, England
- Li S, Li P, Lin G, Chan S, Ho Y 2000. Simultaneous determination of seven major isosteroidal alkaloids in bulb of Fritillaria by gas chromatography. Chromatographia 873(2):221-228.
- Li X, Gao W, Jiang Q, Huang L, Liu C 2011. Study on the morphology crystalline structure, and thermal properties of Fritillaria ussuriensis Maxim starch acetates with different degrees of substitution. Starch-Starke 63(1):24-31.
- Nhut D, Van Le, Fukai S, Tanaka M, Van K 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation 33(1):59-65.
- Perry L, Metzger J 1980. Medicinal plants of East and Southeast Asia: attributed properties. Economic Botany 34(4):361-361.
- Saeed N, Cömertpay S 2017. Activated charcoal improve growth of F. imperialis propagated by indirect organogenesis. Columella. Agriculture Environ., 4:50-64.
- Staikkidou I, Selby C, Hanks G 2000. Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Hortic., 725:421-426.Steinits B, Yahel H 1982. In vitro propagation of Narcissus tazetta. Horticulture Science 17(3):333-334.
- Thomas T 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advance 26(6): 618- 631.
- Wang S, Gao W, Chen H, Xiao P 2005. New starches from Fritillaria species medicinal plants. Carbohydrate polymerases 61(1):111-114.
- Ziv M, Lilien-Kipnis H 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant biology and morphogenesis 19(9):845-850.