1. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
  2. Arşiv
  3. Cilt 23, Sayı 6
Yıl 2020, Cilt 23 , Sayı 6, Sayfalar 1489 - 1494 2020-12-31

Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells
Astaksantinin İnsan Akciğer ve Kolon Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Selim DEMİR [1] , Elif AYAZOGLU DEMİR [2] , Yüksel ALİYAZICIOĞLU [3]

Öz

Astaxanthin (ASX) is a red xanthophyll carotenoid found in various microorganisms and marine animals. ASX is also called the "super antioxidant" because it has the highest antioxidant activity among existing carotenoids. Studies have shown not only antioxidant properties but also antimicrobial, immunomodulatory, hepatoprotective, anticancer and antidiabetic properties of ASX. However, there is a limited number of studies examining the selective cytotoxic effects of ASX on cancer cells. The aim of this study was to determine the cytotoxic effects of ASX on cells representing common cancer types. For this, human breast (MCF-7), lung (A549), liver (HepG2), melanoma (VMM917), colon (WiDr) cancer and normal fibroblast cells were treated with different concentrations of ASX for 72 h and then the MTT assay protocol was applied. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that ASX had a dose-dependent cytotoxic effect on all studied cancer cell lines. However, the strongest selective cytotoxic effect of ASX was determined in A549 and WiDr cells compared to fibroblast cells. This study shows that selective cytotoxic effect of ASX should be investigated more extensively, especially in terms of lung and colon cancer.
Astaksantin (ASX), çeşitli mikroorganizmalarda ve deniz hayvanlarında bulunan kırmızı renkli bir ksantofil karotenoididir. ASX, mevcut karotenoidler arasında en yüksek antioksidan aktiviteye sahip olduğundan "süper antioksidan" olarak da adlandırılmaktadır. ASX’in antioksidan özelliğinin yanında, antimikrobiyal, immünomodülatör, hepatoprotektif, antikanser ve antidiyabetik özellikleri de yapılan çalışmalarla gösterilmiştir. Bununla birlikte, ASX'in kanser hücreleri üzerindeki seçici sitotoksik etkilerini inceleyen çalışmalar sınırlıdır. Bu çalışmanın amacı, ASX'in yaygın kanser türlerini temsil eden hücreler üzerindeki sitotoksik etkilerini belirlemektir. Bunun için insan meme (MCF-7), akciğer (A549), karaciğer (HepG2), melanoma (VMM917), kolon (WiDr) kanseri ve normal fibroblast hücreleri 72 saat boyunca farklı konsantrasyonlarda ASX ile muamele edildi ve ardından MTT protokolü uygulandı. Cisplatin sitotoksisite deneylerinde pozitif kontrol olarak kullanıldı. Sonuçlar, ASX'in incelenen tüm kanser hücre hatları üzerinde doza bağımlı bir sitotoksik etkiye sahip olduğunu gösterdi. Bununla birlikte, fibroblast hücreleri ile kıyaslandığından ASX'in en güçlü seçici sitotoksik etkisinin A549 ve WiDr hücrelerinde olduğu belirlendi. Bu çalışma ASX'in seçici sitotoksik etkisinin, özellikle akciğer ve kolon kanseri açısından daha kapsamlı şekilde araştırılması gerektiğini göstermektedir.

Kaynakça

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Ayrıntılar

Birincil Dil en
Konular Temel Bilimler, Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Orcid: 0000-0002-1863-6280
Yazar: Selim DEMİR (Sorumlu Yazar)
Kurum: Karadeniz Teknik Üniversitesi Sağlık Bilimleri Fakültesi Beslenme ve Diyetetik Bölümü
Ülke: Turkey

Orcid: 0000-0001-9027-7633
Yazar: Elif AYAZOGLU DEMİR
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey

Orcid: 0000-0001-9474-4307
Yazar: Yüksel ALİYAZICIOĞLU
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey
Tarihler

Başvuru Tarihi : 1 Nisan 2020
Kabul Tarihi : 14 Mayıs 2020
Yayımlanma Tarihi : 31 Aralık 2020

Kaynak Göster

APA Demi̇r, S , Ayazoglu Demi̇r, E , Ali̇yazıcıoğlu, Y . (2020). Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1489-1494 . DOI: 10.18016/ksutarimdoga.vi.712905
Makalenin Yazarları
Selim DEMİR [1]
Elif AYAZOGLU DEMİR [2]
Yüksel ALİYAZICIOĞLU [3]
Makale Gönder
Kabul Edilmiş Makaleler
Arşiv
Editör Kurulu
İletişim
Emerging Sources Citation Index (ESCI)
Emerging Sources Citation Index (ESCI)
TR Dizin
TR Dizin