Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

Mehmet Göse; Nurcihan Hacıoğlu

doi:10.18016/ksutarimdoga.v24i60916.750034

Araştırma Makalesi

Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species

Yıl 2021, Cilt: 24 Sayı: 3, 479 - 487, 30.06.2021

Mehmet Göse Nurcihan Hacıoğlu

https://doi.org/10.18016/ksutarimdoga.v24i60916.750034

Cited By: 3

Öz

Methanol, acetone and ethyl acetate extracts obtained from Verbascum pinnatifidum Vahl. and V. antinori Boiss. et Heldr were investigated for their bioactive composition, antimicrobial and antibiofilm activity. Antimicrobial and antibiofilm activity was determined with Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Staphylococcus haemolyticus ATCC 43252 and Candida albicans ATCC 10231 by the disk diffusion, minimum inhibitory concentration and minimum bactericidal or fungicidal concentration and microplate biofilm method, respectively. Bioactive compounds analyses reveal the presence of coumarins, cardiac glycosides, quinones, flavanones. It was revealed that extracts of V. antinori has more antimicrobial activity than V. pinnatifidum extracts against test microorganisms. The lowest MIC value was recorded by V. pinnatifidum methanol extract against A. baumanii ATCC 19606 (0.3125 ± 0.01). Antibiofilm activities of three extracts of V. pinnatifidum and V. antinori have been showed an inhibition percentage range of 8.93 - 92.18% and 14.56 - 91.19%, respectively.

Anahtar Kelimeler

Verbascum pinnatifidum Vahl., V. antinori Boiss. et Heldr., Bioactive Compounds, Antimicrobial, Antibiofilm Activity

Destekleyen Kurum

Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey

Proje Numarası

FYL-2018-2693

Teşekkür

This investigation is a part of Master thesis of Mehmet GÖSE. This study was financially supported by the Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Turkey (FYL-2018-2693). Authors would like to thank Assoc. Prof. Dr. Ersin KARABACAK and Assoc. Prof. Dr. Neslihan DEMİR for defining plant species and helping phytochemical screening, respectively.

Kaynakça

Alimpić A, Knežević A, Šavikin K, Ćurčić M, Veličković D, Stević T, Duletić-Laušević S 2016. Composition and Biological Activities of Different Extracts of Salvia jurisicii, a Rare and Endemic Macedonian Ssecies. Plant Biosystems - An International Journal Dealing with All Aspects of Plant Biology 151(6): 1002–1011.
Barry A 2007. An Overview of the Clinical and Laboratory Standards Institute (CLSI) and Its Impact on Antimicrobial Susceptibility Tests. Antimicrobial Susceptibility Testing Protocols, 1–6.
Baytop T 1999. Türkiye'de Bitkiler ile Tedavi. (2nd ed). İstanbul: Nobel Tıp Kitabevleri.
Boga M, Ertas A, Yilmaz MA, Kizil M, Ceken B, Hasimi N, Ozden TY, Demirci S, Yener I, Deveci O 2016. UHPLC-ESI- MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil of Verbascum pinetorum with Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effects. Iranian Journal of Pharmaceutical Research 15(3): 393-405.
Collins CH, Grange JM, Lyne PM 1989. Collins and Lyne’s Microbiological Methods (6th ed.) London: Butterworths.
Davis PH, Mill RR, Tan K, Genista L. In: Davis, P. H., Mill RR, Tan K, editors. 1988. Flora of Turkey and the East Aegean Islands (Suppl). Edinburgh Univ. Press.
Dulger B, Kirmizi S, Arslan H, Güleryüz G 2002. Antimicrobial Activity of Three Endemic Verbascum Species. Pharmaceutical Biology 40(8): 587–589.
Dulger B, Gonuz A 2004. Antimicrobial Activity of Some Endemic Verbascum, Salvia, and Stachys Species. Pharmaceutical Biology 42(4-5): 301–304.
Dulger B, Ugurlu E, Aki C, Suerdem TB, Camdeviren A, Tazeler G 2005. Evaluation of Antimicrobial Activity of Some Endemic Verbascum, Sideritis, and Stachys Species from Turkey. Pharmaceutical Biology 43(3): 270–274.
Dulger B, Hacioglu N 2008. Antimicrobial Activity of Some Endemic Verbascum and Scrophularia Species from Turkey. Asian Journal of Chemistry 20(5): 3779-3785.
Dulger B, Hacioglu N 2009. Activity of Three endemic Verbascum Species Against Hospital Isolates of Methicillin-Resistant Staphylococcus aureus. Biotechnology and Biotechnological Equipment 23(sup1): 760–762.
Dulger B, Dulger G 2018. Antibacterial Activity of Verbascum antinori. Konuralp Tıp Dergisi 387–390.
Erdönmez D, Kenar N, Türkmen K 2018. Screening for Anti-quorum Sensing and Anti-Biofilm Activity in Viscum album L. Extracts and its Biochemical Composition. Trakya University Journal of Natural Sciences 19(2): 175- 186.
Evans WC 2009. Pharmacognosy (16th ed.) Singapore: W.B. Saunders Co. Ltd.
Georgiev M, Alipieva K, Orhan I, Abrashev R, Denev P, Angelova M 2011. Antioxidant and Cholinesterases Inhibitory Activities of Verbascum xanthophoeniceum Griseb. and Its Phenylethanoid Glycosides. Food Chemistry 128:100–105.
Guarino C 2002. Antimicrobial Activity of Verbascum macrurum Ten. (Scrophulariaceae). Bollettino Chimico Farmaceutico 141(3): 238-242.
Grant SS, Hung DT 2013. Persistent Bacterial Infections, Antibiotic Tolerance, and the Oxidative Stress Response. Virulence 4(4): 273–283.
Harborne JB, Baxter H 1999. Handbook of Natural Flavonoids. Wiley: Chichester.
Kahraman C, Tatlı II, Orhan IE, Akdemir ZS 2010. Cholinesterase Inhibitory and Antioxidant Properties of Verbascum mucronatum Lam. and Its Secondary Metabolites. Zeitschrift Für Naturforschung C 65(11-12): 667–674.
Khan NH, Rahman M, Nur-e-Kamal MSA 1988. Antibacterial Activity of Euphorbia thymifolia Linn. The Indian Journal of Medical Research 87(4):395–397.
Kahraman C, Ekizoglu M, Kart D, Akdemir ZS, Tatli II 2011. Antimicrobial Activity of Some Verbascum Species Growing in Turkey. Fabad Journal of Pharmaceutical Sciences 36: 11-15.
Kozan E, Çankaya IT, Kahraman C, Akkol EK, Akdemir Z 2011. The In vivo Anthelmintic Efficacy of Some Verbascum Species Growing in Turkey. Experimental Parasitology 129(2): 211–214.
Mahindru SN 1992. Indian Plant Perfumes. Metropolitan Phototype Settera Printers Ltd. India.
Merritt JH, Kadouri DE, O’Toole GA 2005. Growing and Analyzing Static Biofilms. Current Protocols in Microbiology. 1(1):1B.
Meurer-Grimes B, McBeth DL, Hallihan B, Delph S 1996. Antimicrobial Activity in Medicinal Plants of the Scrophulariaceae and Acanthaceae. International Journal of Pharmacognosy 34(4): 243–248.
Moghaddam HK, Mirzaii M, Khaksari M, Fazli M, Rahimi F, Behzadi AA 2015. Antibacterial and Anti-adherent Activity of Great Mullein (Verbascum thapsus L.) Ethanolic Extract on In vitro Biofilm Formation of Three Oral Streptococci. International Journal of Health Studies 1(2): 34-37.
Ozcan B, Esen M, Calıskan M, Mothana RA, Cihan AC, Yolcu H 2011. Antimicrobial and Antioxidant Activities of the Various Extracts of Verbascum pinetorum Boiss. O. Kuntze (Scrophulariaceae). European Review for Medical and Pharmacological Sciences 15(8): 900- 905.
Packiavathy SVIA, Agilandeswari P, Musthafa KS, Pandian KS, Ravi VA 2012. Antibiofilm and Quorum Sensing Inhibitory Potential of Cuminum cyminum and Its Secondary Metabolite Methyl Eugenol Against Gram Negative Bacterial Pathogens. Food Research International 45(1): 85–92.
Prakash V, Rana S, Sagar A 2016. Studies on Antibacterial Activity of Verbascum thapsus. Journal of Medicinal Plants Studies 4(3): 101-103.
Pratiwi SUT, Lagendıjk EL, Hertiani T, Weert S, Cornellius AMJ, Vand Den Hondel J 2015. Antimicrobial Effect of Indonesian Medicinal Plants Extracts on Planktonic and Biofilm Growth of Pseudomonas aeruginosa and Staphylococcus aureus. International Journal of Pharmacy and Pharmaceutical Sciences 7(4): 183-191.
Pulcini C, Bush K, Craig WA, Frimodt-Møller N, Grayson ML, Mouton JW 2012. Forgotten Antibiotics: An inventory in Europe, the United States, Canada, and Australia. Clinical Infectious Diseases 54(2): 268–274.
Raaman N 2006. Phytochemical Techniques. New Delhi: New India Publishing Agency.
Sánchez E, Rivas Morales C, Castillo S, Leos-Rivas C, García-Becerra L, Ortiz Martínez DM 2016. Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts Against Nosocomial Microorganisms. Evidence-Based Complementary and Alternative Medicine 2016: 1–8.
Tatli II, Akdemir ZS 2006. Traditional Uses and Biological Activities of Verbascum Species. Fabad Journal of Pharmaceutical Sciences 31: 85-96.
Teanpaisan R, Kawsud P, Pahumunto N, Puripattanavong J 2017. Screening for Antibacterial and Antibiofilm Activity in Thai Medicinal Plant Extracts Against Oral Microorganisms. Journal of Traditional and Complementary Medicine 7(2): 172–177.
Vuong C, Kocianova S, Voyich JM, Yao Y, Fischer ER, DeLeo FR, Otto M 2004. A Crucial Role for Exopolysaccharide Modification in Bacterial Biofilm Formation, Immune Evasion, and Virulence. Journal of Biological Chemistry 279(52): 54881–54886.

İki Verbascum Türünün Biyoaktif Bileşenleri, Antimikrobiyal ve Antibiyofilm Aktiviteleri

Yıl 2021, Cilt: 24 Sayı: 3, 479 - 487, 30.06.2021

Mehmet Göse Nurcihan Hacıoğlu

https://doi.org/10.18016/ksutarimdoga.v24i60916.750034

Cited By: 3

Öz

Verbascum pinnatifidum Vahl. ve V. antinori Boiss. et Heldr’den elde edilen methanol, aseton ve etil asetat ekstraktları bioaktif bileşenleri, antimikrobiyal ve antibiyofilm aktiviteleri bakımından araştırıldı. Antimikrobiyal ve antibiyofilm aktiviteleri Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606), Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Staphylococcus haemolyticus ATCC 43252 ve Candida albicans ATCC 10231 ile sırasıyla disk difüzyon, minimum inhibitör konsantrasyonu ve minimum bakterisidal veya fungisidal konsantrasyonu ve mikroplak biyofilm metodu ile belirlendi. Biyoaktif bileşik analizleri kumarinler, kardiak glikozidler, kuinonlar ve flavanonların varlığını ortaya koymuştur. V. antinori ekstraktlarının test mikroorganizmalarına karşı V. pinnatifidum ekstraktlarından daha yüksek antimikrobiyal aktivite sahip olduğu ortaya çıkmıştır. En düşük MIK değeri A. baumanii ATCC 19606 (0.3125 ± 0.01)’ya karşı V. pinnatifidum methanol ekstraktı ile kaydedilmiştir. Üç V. pinnatifidum ve V. antinori ekstraktının antibiyofilm aktiviteleri sırasıyla %8.93 - 92.18 ve %14.56 - 91.19 oranlarında inhibisyon yüzdesi göstermiştir.

Anahtar Kelimeler

Verbascum pinnatifidum Vahl., V. antinori Boiss. et Heldr., Bioaktif Bileşikler, Antimikrobiyal, Antibiyofilm Aktivite

Proje Numarası

FYL-2018-2693

Kaynakça

Alimpić A, Knežević A, Šavikin K, Ćurčić M, Veličković D, Stević T, Duletić-Laušević S 2016. Composition and Biological Activities of Different Extracts of Salvia jurisicii, a Rare and Endemic Macedonian Ssecies. Plant Biosystems - An International Journal Dealing with All Aspects of Plant Biology 151(6): 1002–1011.
Barry A 2007. An Overview of the Clinical and Laboratory Standards Institute (CLSI) and Its Impact on Antimicrobial Susceptibility Tests. Antimicrobial Susceptibility Testing Protocols, 1–6.
Baytop T 1999. Türkiye'de Bitkiler ile Tedavi. (2nd ed). İstanbul: Nobel Tıp Kitabevleri.
Boga M, Ertas A, Yilmaz MA, Kizil M, Ceken B, Hasimi N, Ozden TY, Demirci S, Yener I, Deveci O 2016. UHPLC-ESI- MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil of Verbascum pinetorum with Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effects. Iranian Journal of Pharmaceutical Research 15(3): 393-405.
Collins CH, Grange JM, Lyne PM 1989. Collins and Lyne’s Microbiological Methods (6th ed.) London: Butterworths.
Davis PH, Mill RR, Tan K, Genista L. In: Davis, P. H., Mill RR, Tan K, editors. 1988. Flora of Turkey and the East Aegean Islands (Suppl). Edinburgh Univ. Press.
Dulger B, Kirmizi S, Arslan H, Güleryüz G 2002. Antimicrobial Activity of Three Endemic Verbascum Species. Pharmaceutical Biology 40(8): 587–589.
Dulger B, Gonuz A 2004. Antimicrobial Activity of Some Endemic Verbascum, Salvia, and Stachys Species. Pharmaceutical Biology 42(4-5): 301–304.
Dulger B, Ugurlu E, Aki C, Suerdem TB, Camdeviren A, Tazeler G 2005. Evaluation of Antimicrobial Activity of Some Endemic Verbascum, Sideritis, and Stachys Species from Turkey. Pharmaceutical Biology 43(3): 270–274.
Dulger B, Hacioglu N 2008. Antimicrobial Activity of Some Endemic Verbascum and Scrophularia Species from Turkey. Asian Journal of Chemistry 20(5): 3779-3785.
Dulger B, Hacioglu N 2009. Activity of Three endemic Verbascum Species Against Hospital Isolates of Methicillin-Resistant Staphylococcus aureus. Biotechnology and Biotechnological Equipment 23(sup1): 760–762.
Dulger B, Dulger G 2018. Antibacterial Activity of Verbascum antinori. Konuralp Tıp Dergisi 387–390.
Erdönmez D, Kenar N, Türkmen K 2018. Screening for Anti-quorum Sensing and Anti-Biofilm Activity in Viscum album L. Extracts and its Biochemical Composition. Trakya University Journal of Natural Sciences 19(2): 175- 186.
Evans WC 2009. Pharmacognosy (16th ed.) Singapore: W.B. Saunders Co. Ltd.
Georgiev M, Alipieva K, Orhan I, Abrashev R, Denev P, Angelova M 2011. Antioxidant and Cholinesterases Inhibitory Activities of Verbascum xanthophoeniceum Griseb. and Its Phenylethanoid Glycosides. Food Chemistry 128:100–105.
Guarino C 2002. Antimicrobial Activity of Verbascum macrurum Ten. (Scrophulariaceae). Bollettino Chimico Farmaceutico 141(3): 238-242.
Grant SS, Hung DT 2013. Persistent Bacterial Infections, Antibiotic Tolerance, and the Oxidative Stress Response. Virulence 4(4): 273–283.
Harborne JB, Baxter H 1999. Handbook of Natural Flavonoids. Wiley: Chichester.
Kahraman C, Tatlı II, Orhan IE, Akdemir ZS 2010. Cholinesterase Inhibitory and Antioxidant Properties of Verbascum mucronatum Lam. and Its Secondary Metabolites. Zeitschrift Für Naturforschung C 65(11-12): 667–674.
Khan NH, Rahman M, Nur-e-Kamal MSA 1988. Antibacterial Activity of Euphorbia thymifolia Linn. The Indian Journal of Medical Research 87(4):395–397.
Kahraman C, Ekizoglu M, Kart D, Akdemir ZS, Tatli II 2011. Antimicrobial Activity of Some Verbascum Species Growing in Turkey. Fabad Journal of Pharmaceutical Sciences 36: 11-15.
Kozan E, Çankaya IT, Kahraman C, Akkol EK, Akdemir Z 2011. The In vivo Anthelmintic Efficacy of Some Verbascum Species Growing in Turkey. Experimental Parasitology 129(2): 211–214.
Mahindru SN 1992. Indian Plant Perfumes. Metropolitan Phototype Settera Printers Ltd. India.
Merritt JH, Kadouri DE, O’Toole GA 2005. Growing and Analyzing Static Biofilms. Current Protocols in Microbiology. 1(1):1B.
Meurer-Grimes B, McBeth DL, Hallihan B, Delph S 1996. Antimicrobial Activity in Medicinal Plants of the Scrophulariaceae and Acanthaceae. International Journal of Pharmacognosy 34(4): 243–248.
Moghaddam HK, Mirzaii M, Khaksari M, Fazli M, Rahimi F, Behzadi AA 2015. Antibacterial and Anti-adherent Activity of Great Mullein (Verbascum thapsus L.) Ethanolic Extract on In vitro Biofilm Formation of Three Oral Streptococci. International Journal of Health Studies 1(2): 34-37.
Ozcan B, Esen M, Calıskan M, Mothana RA, Cihan AC, Yolcu H 2011. Antimicrobial and Antioxidant Activities of the Various Extracts of Verbascum pinetorum Boiss. O. Kuntze (Scrophulariaceae). European Review for Medical and Pharmacological Sciences 15(8): 900- 905.
Packiavathy SVIA, Agilandeswari P, Musthafa KS, Pandian KS, Ravi VA 2012. Antibiofilm and Quorum Sensing Inhibitory Potential of Cuminum cyminum and Its Secondary Metabolite Methyl Eugenol Against Gram Negative Bacterial Pathogens. Food Research International 45(1): 85–92.
Prakash V, Rana S, Sagar A 2016. Studies on Antibacterial Activity of Verbascum thapsus. Journal of Medicinal Plants Studies 4(3): 101-103.
Pratiwi SUT, Lagendıjk EL, Hertiani T, Weert S, Cornellius AMJ, Vand Den Hondel J 2015. Antimicrobial Effect of Indonesian Medicinal Plants Extracts on Planktonic and Biofilm Growth of Pseudomonas aeruginosa and Staphylococcus aureus. International Journal of Pharmacy and Pharmaceutical Sciences 7(4): 183-191.
Pulcini C, Bush K, Craig WA, Frimodt-Møller N, Grayson ML, Mouton JW 2012. Forgotten Antibiotics: An inventory in Europe, the United States, Canada, and Australia. Clinical Infectious Diseases 54(2): 268–274.
Raaman N 2006. Phytochemical Techniques. New Delhi: New India Publishing Agency.
Sánchez E, Rivas Morales C, Castillo S, Leos-Rivas C, García-Becerra L, Ortiz Martínez DM 2016. Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts Against Nosocomial Microorganisms. Evidence-Based Complementary and Alternative Medicine 2016: 1–8.
Tatli II, Akdemir ZS 2006. Traditional Uses and Biological Activities of Verbascum Species. Fabad Journal of Pharmaceutical Sciences 31: 85-96.
Teanpaisan R, Kawsud P, Pahumunto N, Puripattanavong J 2017. Screening for Antibacterial and Antibiofilm Activity in Thai Medicinal Plant Extracts Against Oral Microorganisms. Journal of Traditional and Complementary Medicine 7(2): 172–177.
Vuong C, Kocianova S, Voyich JM, Yao Y, Fischer ER, DeLeo FR, Otto M 2004. A Crucial Role for Exopolysaccharide Modification in Bacterial Biofilm Formation, Immune Evasion, and Virulence. Journal of Biological Chemistry 279(52): 54881–54886.

Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Yapısal Biyoloji
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Mehmet Göse 0000-0002-6029-2442 Nurcihan Hacıoğlu 0000-0002-5812-9398
Proje Numarası	FYL-2018-2693
Yayımlanma Tarihi	30 Haziran 2021
Gönderilme Tarihi	9 Haziran 2020
Kabul Tarihi	30 Eylül 2020
Yayımlandığı Sayı	Yıl 2021Cilt: 24 Sayı: 3

Kaynak Göster

APA	Göse, M., & Hacıoğlu, N. (2021). Bioactive Compounds, Antimicrobial and Antibiofilm Activity of Two Verbascum Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(3), 479-487. https://doi.org/10.18016/ksutarimdoga.v24i60916.750034