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Antioxidant and Cytotoxic Activities of Fumaria parviflora Lam. and Fumaria capreolata L.

Yıl 2022, Cilt 25, Sayı 4, 819 - 827, 31.08.2022
https://doi.org/10.18016/ksutarimdoga.vi.899937

Öz

This study was carried out to examine the in vitro antioxidant and cytotoxic effects of methanol extracts of Fumaria parviflora Lam. and Fumaria capreolata. Antioxidant assays carried out by employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and phosphomolybdate assays. Cell viability was quantified by employing MTT assay for 24, 48 and 72 h of exposures to 25-250 µgmL-1 concentrations of the extracts. F. parviflora methanol extract possessed the highest antioxidant activities in both free DPPH scavenging and total antioxidant capacity assays. Results from MTT assay revealed that F. capreolata methanol extract significantly increased the cell proliferation in human hepatocellular carcinoma (Hep3B) cells after 24 and 48 hours of treatment. After 72 hours of treatment, methanol extract slightly inhibited the proliferation of Hep3B cell lines, in a time-and dose-dependent manner. While F. parviflora extract had the highest cytotoxic effect on Hep3B cells after 72 hours of the treatment. F. capreolata did not cause any significant cytotoxic effect on these cell lines.

Kaynakça

  • Acay H, Dündar A, Kaçar S, Baran MF, Keskin C 2021. Investigation of nutritional content, antioxidant, anticancer and antimicrobial activities of Pleurotus eryngii (DC. Ex Fr.) Quel, extract obtained by different solvents. KSU J Agric Nat 24(2): 267-277.
  • Adewusi EA, Afolayan AJ 2010. A review of natural products with hepatoprotective activity. Journal Medicinal Plants Research 4: 1318-1334.
  • Atlas of Living Australia [Internet], Avaible from: https://bie.ala.org.au/species (accessed February 1, 2021).
  • Blois MS 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.
  • Bribi N, Algieri F, Rodriguez-Nogales A, Garrido-Mesa J, Vezza T, Maiza F, Utrilla MP, Rodriguez-Cabezas ME, Galvez J 2015. Anti-nociceptive and anti-inflammatory effects of total alkaloid extract from Fumaria capreolata. Evidence Based Complementary and Alternative Medicine 736895: 1-8.
  • Brand-Williams W, Cuvelier ME, Berset C 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm.-Wiss. u.-Technol 28: 25-30.
  • Bribi N, Yavine B, Fadila MB 2013. Evaluation of erythrocytes toxicity and antioxidant activity of alkaloids of Fumaria capreolata. Int J Pharma and Bio Sci 4: 770-776.
  • Calabrese EJ, Mattson MP 2017. How does hormesis impact biology, toxicology, and medicine? NPJ Aging Mech Dis 3 (1): 1-8.
  • Chaves N, Santiago A, Alias JC 2020. Quantification of the antioxidant activity of plant extracts: analysis of sensitivity and hierarchization based on the method used. Antioxidants (Basel) 9: 76.
  • Cullen J 1965. Flora of Turkey and East Aegean Islands. (Ed. Davis, PH. Edinburgh University Press. Dorostghoal M, Seyyednejad SM, Jabari A 2013. Protective effects of Fumaria parviflora L. on lead-induced testicular toxicity in male rats. Andrologia 46: 437-446.
  • Duthie GG, Duthie SJ, Kyle JAM 2000. Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr Res Rev 13:79-106.
  • Ezzat M, Kian A, Bagher M, Seyedeh ZOS 2012. Protective activity of Fumaria vaillantii Loisel and mono methyl fumrate on acetoaminophen induced hepatotoxicity in mice. Int J Pharmacology 8: 177-184.
  • Fafal T, Önür MA 2007. Determination of protopine in Fumaria densiflora DC. by TLC- densitometric and spectrophotometric method. Journal of Faculty of Pharmacy 4: 223 – 235.
  • Gao Y, Su Y, Qu L, Xu S, Meng L, Cai SQ, Shou C 2011. Mitochondrial apoptosis contributes to the anti-cancer effect of Smilax glabra Roxb. Toxicol Lett 207:112-120.
  • Ghafoor K, Choi YH 2009. Optimization of ultrasound-assisted extraction of phenolic compounds and antioxidants from grape peels through response surface methodology. Journal of Korean Society for Applied Biological Chemistry 52: 295-300.
  • Gilani AH, Janbaz KH, Akhtar MS 1996. Selective protective effect of an extract from Fumaria parviflora on paracetamol-induced hepatotoxicity. Gen Pharmacol 27: 979-983.
  • Güzel Y, Güzelşemme M, Miski M 2015. Ethnobotany of medicinal plants used in Antakya: a multicultural district in Hatay Province of Turkey. Journal of Ethnopharmacology 174: 118-152.
  • Heidari RM, Mandgary A, Enayati M 2004. Antinociceptive effects and toxicity of Fumaria parviflora Lam. in mice and rats. Daru Journal of Pharmaceutical Sciences 12: 136-140.
  • Ho WC, Zhang J 2018. Evolutionary adaptations to new environments generally reverse plastic phenotypic changes. Nat Commun 9 (1): 1-11.
  • Humayun S, Ibrar M, Barkatullah AI 2012. Comparison of three extracts of Fumaria indica for the evaluation of cytotoxic and phytotoxic activities. Int J Biosci 2: 112-119.
  • Hussain T, Hefazat HS, Sheeba F, Sweety K, Vijayakumar M, Rao CV 2012. Chemopreventive effect of Fumaria indica that modulates the oxidant antioxidant inbalance during N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wistar rats. Asian Pacific Journal of Tropical Biomedicine 2: 995-1001.
  • Jalal A, de Oliveira Junior JC, Ribeiro JS et al 2021. Hormesis in plants: Physiological and biochemical responses. Ecotoxicology and Environmental Safety 207: 111225.
  • Jameel M, Ali A, Ali M 2014. New phytoconstituents from the aerial parts of Fumaria parviflora Lam. J Adv Pharm Technol Res 5: 64-69.
  • Jamshidzadeh A, Niknahad H 2006. Hepatoprotective effects of Fumaria parviflora L. on CCl4-induced hepatotoxicity. J Med Plants 19: 34-39.
  • Jodynis-Liebhert J, Kujawska M 2020. Biphasic dose-response induced by phytochemicals: Experimental evidence. Journal of Clinical Medicine 9: 718.
  • Kumar S, Kamboj A, Sharma AK 2018. Antioxidant evaluation of ethanolic extract of Fumaria parviflora Lam. obtained from root, stem, leaf and fruit and measurement of their total phenols and flavonoids. The Pharma Innovation Journal 7: 577-579.
  • Kumar S, Kamboj A, Sharma AK 2018. Pharmacognostic Standardization of roots, stems, leaves and fruits of Fumaria parviflora Lam. (Fumariaceae). Asian Journal of Pharmacy and Pharmacology 4: 238-244.
  • Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB 2014. Resources and biological activities of natural polyphenols. Nutrients 6: 6020-6047.
  • Maiza-Benbdesselam F, Khentache S, Bougoffa K, Chibane M, Adach S, Chapeleur Y, Max H, Laurain-Mattar D 2007. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria : Fumaria capreolata and Fumaria bastardii. Rec. Natural Prod 1: 28-35.
  • Moghaddam M, Khaleghi Miran SN, Mehdizadeh L 2018. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. International Journal of Horticultural Science and Technology 5: 93-102.
  • Mossman T 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55-63.
  • Nabavizadeh SH, Majid OB, Anushiravani A 2009. Direct ex vivo herbal extracts on serum bilirubin in neonatal blood samples. African Journal Biochemistry Research 3: 226-228.
  • Nemzer BV, Yashin YA, Vedenin AN, Yashin YI, Yashunsky DV, Nifantiev NE, Kalta D 2019. Selected powerful natural antioxidants: Structure, food sources, antioxidant activities and important health benefits. J Food Res 8: 60-79.
  • Paltinean R, Toiu A, Wauters JN, Frederich M, Tits M, Angenot L, Tamas M, Crisan G 2013. Identification and determination of alkaloids in species from Fumaria species from Romania. Digest Journal of Nanomaterials and Biostructures 8: 817-824.
  • Qui GH, Xie X, Xu F, Shi X, Wang Y, Deng L 2015. Distinctive pharmacological differences between liver cancer cell lines HepG2 and Hep3B. Cytotechnology 67:1-12.
  • Rathi A, Srivastava A, Shirwaikar A, Rawat A 2008. Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine 15: 470-477.
  • Raza A, Sood GK 2014. Hepatocellular carcinoma review: current treatment, and evidence-based medicine. World J Gastroenterol 21: 4115-27.
  • Seyed RM, Nassiri-Asl M, Farahani-Nick Z, Savad S, Seyed KF 2007. Protective effects of Fumaria vaillantii extract on carbon tetrachloride induced hepatotoxicity in rats. Pharmacologyonline 3: 385-393.
  • Sheldon JW, Balick MJ, Laird SA 1997. Medicinal plants: can utilization and conservation coexist? Advances in economic botany, The New York Botanical Garden. New York, Bronx.
  • Siddiqui S, Ahmad E, Gupta M, Rawat V, Shivnath N, Banerjee M, Khan MS, Arshad M 2015. Cissus quadrangularis Linn exerts dose-dependent biphasic effects: osteogenic and anti-proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblasts. Cell Proliferation 48: 443-454.
  • Tabrizi FHA, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H, Salimi M 2016. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Research in Pharmaceutical Sciences 11: 152-159.
  • Triphati M, Singh BK, Mishra C, Raisuddin S, Kakkar P 2010. Involvement of mitochondria mediated pathways in hepatoprotection conferred by Fumaria parviflora Lam. extract against nimesulide induced apoptosis in vitro. Toxicology In Vitro 24: 495-508.
  • Zhang R, Guo Q, Kennelly EJ, Long C, Chai X 2020. Diverse alkaloids and biological activities of Fumaria (Papaveraceae): An ethnomedicinal group. Fitoterapia 146: 104697.

Fumaria parviflora Lam. ve Fumaria capreolata L.’nin Antioksidan ve Sitotoksik Aktiviteleri

Yıl 2022, Cilt 25, Sayı 4, 819 - 827, 31.08.2022
https://doi.org/10.18016/ksutarimdoga.vi.899937

Öz

Bu çalışmada, Fumaria parviflora ve Fumaria capreolata metanol özütlerinin in vitro antioksidan aktiviteleri ile iki farklı insan hepatosellüler karsinoma (Hep3B ve HepG2) hücre hattı üzerindeki sitotoksik etkileri araştırılmıştır. Özütlerin antioksidan aktiviteleri 1,1-diphenyl-2-picrylhydrazyl (DPPH) radikalini süpürme ve fosfomolibdat testi ile belirlenmiştir. Hücre canlılığı ise 24, 48 ve 72 saatlik farklı özüt konsantrasyonları (25-250 µgmL-1) ile muamele sonrasında MTT testi ile belirlenmiştir. F. parviflora ve F. capreolata metanol özütleri serbest DPPH süpürme aktivitesi ve toplam antioksidan kapasitesi açısından karşılaştırıldığında, F. parviflora metanol özütü hem DPPH serbest radikalini süpürme hem de toplam antioksidan kapasitesi bakımından yüksek aktiviteye sahiptir. MTT assay sonuçları, F. capreolata metanol özütünün 24 ve 48 saatlik muameleden sonra Hep3B hücrelerinde hücre çoğalmasını önemli ölçüde artırdığını ortaya çıkarmıştır. 72 saatlik uygulama sonrası, metanol özütü Hep3B hücrelerinin çoğalmasını zamana ve konsantrasyona bağlı şekilde hafif inhibe etmiştir. F. parviflora özütü, Hep3B hücreleri üzerinde sadece 72 saatlik uygulamada yüksek sitotoksik etkiye sahipken, F. capreolata bu hücreler üzerinde önemli bir sitotoksik etki göstermemiştir.

Kaynakça

  • Acay H, Dündar A, Kaçar S, Baran MF, Keskin C 2021. Investigation of nutritional content, antioxidant, anticancer and antimicrobial activities of Pleurotus eryngii (DC. Ex Fr.) Quel, extract obtained by different solvents. KSU J Agric Nat 24(2): 267-277.
  • Adewusi EA, Afolayan AJ 2010. A review of natural products with hepatoprotective activity. Journal Medicinal Plants Research 4: 1318-1334.
  • Atlas of Living Australia [Internet], Avaible from: https://bie.ala.org.au/species (accessed February 1, 2021).
  • Blois MS 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.
  • Bribi N, Algieri F, Rodriguez-Nogales A, Garrido-Mesa J, Vezza T, Maiza F, Utrilla MP, Rodriguez-Cabezas ME, Galvez J 2015. Anti-nociceptive and anti-inflammatory effects of total alkaloid extract from Fumaria capreolata. Evidence Based Complementary and Alternative Medicine 736895: 1-8.
  • Brand-Williams W, Cuvelier ME, Berset C 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm.-Wiss. u.-Technol 28: 25-30.
  • Bribi N, Yavine B, Fadila MB 2013. Evaluation of erythrocytes toxicity and antioxidant activity of alkaloids of Fumaria capreolata. Int J Pharma and Bio Sci 4: 770-776.
  • Calabrese EJ, Mattson MP 2017. How does hormesis impact biology, toxicology, and medicine? NPJ Aging Mech Dis 3 (1): 1-8.
  • Chaves N, Santiago A, Alias JC 2020. Quantification of the antioxidant activity of plant extracts: analysis of sensitivity and hierarchization based on the method used. Antioxidants (Basel) 9: 76.
  • Cullen J 1965. Flora of Turkey and East Aegean Islands. (Ed. Davis, PH. Edinburgh University Press. Dorostghoal M, Seyyednejad SM, Jabari A 2013. Protective effects of Fumaria parviflora L. on lead-induced testicular toxicity in male rats. Andrologia 46: 437-446.
  • Duthie GG, Duthie SJ, Kyle JAM 2000. Plant polyphenols in cancer and heart disease: Implications as nutritional antioxidants. Nutr Res Rev 13:79-106.
  • Ezzat M, Kian A, Bagher M, Seyedeh ZOS 2012. Protective activity of Fumaria vaillantii Loisel and mono methyl fumrate on acetoaminophen induced hepatotoxicity in mice. Int J Pharmacology 8: 177-184.
  • Fafal T, Önür MA 2007. Determination of protopine in Fumaria densiflora DC. by TLC- densitometric and spectrophotometric method. Journal of Faculty of Pharmacy 4: 223 – 235.
  • Gao Y, Su Y, Qu L, Xu S, Meng L, Cai SQ, Shou C 2011. Mitochondrial apoptosis contributes to the anti-cancer effect of Smilax glabra Roxb. Toxicol Lett 207:112-120.
  • Ghafoor K, Choi YH 2009. Optimization of ultrasound-assisted extraction of phenolic compounds and antioxidants from grape peels through response surface methodology. Journal of Korean Society for Applied Biological Chemistry 52: 295-300.
  • Gilani AH, Janbaz KH, Akhtar MS 1996. Selective protective effect of an extract from Fumaria parviflora on paracetamol-induced hepatotoxicity. Gen Pharmacol 27: 979-983.
  • Güzel Y, Güzelşemme M, Miski M 2015. Ethnobotany of medicinal plants used in Antakya: a multicultural district in Hatay Province of Turkey. Journal of Ethnopharmacology 174: 118-152.
  • Heidari RM, Mandgary A, Enayati M 2004. Antinociceptive effects and toxicity of Fumaria parviflora Lam. in mice and rats. Daru Journal of Pharmaceutical Sciences 12: 136-140.
  • Ho WC, Zhang J 2018. Evolutionary adaptations to new environments generally reverse plastic phenotypic changes. Nat Commun 9 (1): 1-11.
  • Humayun S, Ibrar M, Barkatullah AI 2012. Comparison of three extracts of Fumaria indica for the evaluation of cytotoxic and phytotoxic activities. Int J Biosci 2: 112-119.
  • Hussain T, Hefazat HS, Sheeba F, Sweety K, Vijayakumar M, Rao CV 2012. Chemopreventive effect of Fumaria indica that modulates the oxidant antioxidant inbalance during N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wistar rats. Asian Pacific Journal of Tropical Biomedicine 2: 995-1001.
  • Jalal A, de Oliveira Junior JC, Ribeiro JS et al 2021. Hormesis in plants: Physiological and biochemical responses. Ecotoxicology and Environmental Safety 207: 111225.
  • Jameel M, Ali A, Ali M 2014. New phytoconstituents from the aerial parts of Fumaria parviflora Lam. J Adv Pharm Technol Res 5: 64-69.
  • Jamshidzadeh A, Niknahad H 2006. Hepatoprotective effects of Fumaria parviflora L. on CCl4-induced hepatotoxicity. J Med Plants 19: 34-39.
  • Jodynis-Liebhert J, Kujawska M 2020. Biphasic dose-response induced by phytochemicals: Experimental evidence. Journal of Clinical Medicine 9: 718.
  • Kumar S, Kamboj A, Sharma AK 2018. Antioxidant evaluation of ethanolic extract of Fumaria parviflora Lam. obtained from root, stem, leaf and fruit and measurement of their total phenols and flavonoids. The Pharma Innovation Journal 7: 577-579.
  • Kumar S, Kamboj A, Sharma AK 2018. Pharmacognostic Standardization of roots, stems, leaves and fruits of Fumaria parviflora Lam. (Fumariaceae). Asian Journal of Pharmacy and Pharmacology 4: 238-244.
  • Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB 2014. Resources and biological activities of natural polyphenols. Nutrients 6: 6020-6047.
  • Maiza-Benbdesselam F, Khentache S, Bougoffa K, Chibane M, Adach S, Chapeleur Y, Max H, Laurain-Mattar D 2007. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria : Fumaria capreolata and Fumaria bastardii. Rec. Natural Prod 1: 28-35.
  • Moghaddam M, Khaleghi Miran SN, Mehdizadeh L 2018. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. International Journal of Horticultural Science and Technology 5: 93-102.
  • Mossman T 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55-63.
  • Nabavizadeh SH, Majid OB, Anushiravani A 2009. Direct ex vivo herbal extracts on serum bilirubin in neonatal blood samples. African Journal Biochemistry Research 3: 226-228.
  • Nemzer BV, Yashin YA, Vedenin AN, Yashin YI, Yashunsky DV, Nifantiev NE, Kalta D 2019. Selected powerful natural antioxidants: Structure, food sources, antioxidant activities and important health benefits. J Food Res 8: 60-79.
  • Paltinean R, Toiu A, Wauters JN, Frederich M, Tits M, Angenot L, Tamas M, Crisan G 2013. Identification and determination of alkaloids in species from Fumaria species from Romania. Digest Journal of Nanomaterials and Biostructures 8: 817-824.
  • Qui GH, Xie X, Xu F, Shi X, Wang Y, Deng L 2015. Distinctive pharmacological differences between liver cancer cell lines HepG2 and Hep3B. Cytotechnology 67:1-12.
  • Rathi A, Srivastava A, Shirwaikar A, Rawat A 2008. Hepatoprotective potential of Fumaria indica Pugsley whole plant extracts, fractions and an isolated alkaloid protopine. Phytomedicine 15: 470-477.
  • Raza A, Sood GK 2014. Hepatocellular carcinoma review: current treatment, and evidence-based medicine. World J Gastroenterol 21: 4115-27.
  • Seyed RM, Nassiri-Asl M, Farahani-Nick Z, Savad S, Seyed KF 2007. Protective effects of Fumaria vaillantii extract on carbon tetrachloride induced hepatotoxicity in rats. Pharmacologyonline 3: 385-393.
  • Sheldon JW, Balick MJ, Laird SA 1997. Medicinal plants: can utilization and conservation coexist? Advances in economic botany, The New York Botanical Garden. New York, Bronx.
  • Siddiqui S, Ahmad E, Gupta M, Rawat V, Shivnath N, Banerjee M, Khan MS, Arshad M 2015. Cissus quadrangularis Linn exerts dose-dependent biphasic effects: osteogenic and anti-proliferative, through modulating ROS, cell cycle and Runx2 gene expression in primary rat osteoblasts. Cell Proliferation 48: 443-454.
  • Tabrizi FHA, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H, Salimi M 2016. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Research in Pharmaceutical Sciences 11: 152-159.
  • Triphati M, Singh BK, Mishra C, Raisuddin S, Kakkar P 2010. Involvement of mitochondria mediated pathways in hepatoprotection conferred by Fumaria parviflora Lam. extract against nimesulide induced apoptosis in vitro. Toxicology In Vitro 24: 495-508.
  • Zhang R, Guo Q, Kennelly EJ, Long C, Chai X 2020. Diverse alkaloids and biological activities of Fumaria (Papaveraceae): An ethnomedicinal group. Fitoterapia 146: 104697.

Ayrıntılar

Birincil Dil Türkçe
Konular Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Tülay AŞKIN ÇELİK> (Sorumlu Yazar)
AYDIN ADNAN MENDERES ÜNİVERSİTESİ
0000-0001-6891-9089
Türkiye


Özlem Sultan ASLANTÜRK>
ADNAN MENDERES ÜNİVERSİTESİ
0000-0002-2503-0164
Türkiye


Ebru Şebnem YILMAZ>
HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
0000-0001-6124-4832
Türkiye


Yelda GÜZEL>
HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
0000-0002-7975-3130
Türkiye

Destekleyen Kurum HMKU Scientific Research Project Fund .
Proje Numarası 18.YL.010
Yayımlanma Tarihi 31 Ağustos 2022
Başvuru Tarihi 19 Mart 2021
Kabul Tarihi 16 Temmuz 2021
Yayınlandığı Sayı Yıl 2022, Cilt 25, Sayı 4

Kaynak Göster

APA Aşkın Çelik, T. , Aslantürk, Ö. S. , Yılmaz, E. Ş. & Güzel, Y. (2022). Fumaria parviflora Lam. ve Fumaria capreolata L.’nin Antioksidan ve Sitotoksik Aktiviteleri . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 25 (4) , 819-827 . DOI: 10.18016/ksutarimdoga.vi.899937
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